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Negative correlation between serum levels of homocysteine and apolipoprotein M

Wei, J.; Yu, Y.; Feng, Y.; Zhang, J.; Jiang, Q.; Zheng, L.; Zhang, X.; Xu, N. LU and Luo, G. (2019) In Current Molecular Medicine 19(2). p.118-124
Abstract

Background: Homocysteine (Hcy) has been suggested as an independent risk factor for atherosclerosis. Apolipoprotein M (apoM) is a constituent of the HDL particles. The goal of this study was to examine the serum levels of homocysteine and apoM and to determine whether homocysteine influences apoM synthesis. Methods: Serum levels of apoM and Hcy in 17 hyperhomocysteinemia (HHcy) patients and 19 controls were measured and their correlations were analyzed. Different concentrations of homocysteine (Hcy) and LY294002, a specific phosphoinositide 3-kinase (PI3K) inhibitor, were used to treat HepG2 cells. The mRNA levels were determined by RT-PCR and the apoM protein mass was measured by western blot. Results: We found that decreased serum... (More)

Background: Homocysteine (Hcy) has been suggested as an independent risk factor for atherosclerosis. Apolipoprotein M (apoM) is a constituent of the HDL particles. The goal of this study was to examine the serum levels of homocysteine and apoM and to determine whether homocysteine influences apoM synthesis. Methods: Serum levels of apoM and Hcy in 17 hyperhomocysteinemia (HHcy) patients and 19 controls were measured and their correlations were analyzed. Different concentrations of homocysteine (Hcy) and LY294002, a specific phosphoinositide 3-kinase (PI3K) inhibitor, were used to treat HepG2 cells. The mRNA levels were determined by RT-PCR and the apoM protein mass was measured by western blot. Results: We found that decreased serum apoM levels corresponded with serum HDL levels in HHcy patients, while the serum apoM levels showed a statistically significant negative correlation with the serum Hcy levels. Moreover, apoM mRNA and protein levels were significantly decreased after the administration of Hcy in HepG2 cells, and this effect could be abolished by addition of LY294002. Conclusions: Present study demonstrates that Hcy downregulates the expression of apoM by mechanisms involving the PI3K signal pathway.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Apolipoprotein M, Cardiovascular diseases, Homocysteine, Hyperhomocysteinemia, Lipid metabolism, PI3K
in
Current Molecular Medicine
volume
19
issue
2
pages
7 pages
publisher
Bentham Science Publishers
external identifiers
  • scopus:85066283674
ISSN
1566-5240
DOI
10.2174/1566524019666190308115624
language
English
LU publication?
yes
id
5e653d41-1010-4d6c-b9af-1064b6404742
date added to LUP
2019-06-13 11:58:37
date last changed
2019-07-02 04:48:01
@article{5e653d41-1010-4d6c-b9af-1064b6404742,
  abstract     = {<p>Background: Homocysteine (Hcy) has been suggested as an independent risk factor for atherosclerosis. Apolipoprotein M (apoM) is a constituent of the HDL particles. The goal of this study was to examine the serum levels of homocysteine and apoM and to determine whether homocysteine influences apoM synthesis. Methods: Serum levels of apoM and Hcy in 17 hyperhomocysteinemia (HHcy) patients and 19 controls were measured and their correlations were analyzed. Different concentrations of homocysteine (Hcy) and LY294002, a specific phosphoinositide 3-kinase (PI3K) inhibitor, were used to treat HepG2 cells. The mRNA levels were determined by RT-PCR and the apoM protein mass was measured by western blot. Results: We found that decreased serum apoM levels corresponded with serum HDL levels in HHcy patients, while the serum apoM levels showed a statistically significant negative correlation with the serum Hcy levels. Moreover, apoM mRNA and protein levels were significantly decreased after the administration of Hcy in HepG2 cells, and this effect could be abolished by addition of LY294002. Conclusions: Present study demonstrates that Hcy downregulates the expression of apoM by mechanisms involving the PI3K signal pathway.</p>},
  author       = {Wei, J. and Yu, Y. and Feng, Y. and Zhang, J. and Jiang, Q. and Zheng, L. and Zhang, X. and Xu, N. and Luo, G.},
  issn         = {1566-5240},
  keyword      = {Apolipoprotein M,Cardiovascular diseases,Homocysteine,Hyperhomocysteinemia,Lipid metabolism,PI3K},
  language     = {eng},
  number       = {2},
  pages        = {118--124},
  publisher    = {Bentham Science Publishers},
  series       = {Current Molecular Medicine},
  title        = {Negative correlation between serum levels of homocysteine and apolipoprotein M},
  url          = {http://dx.doi.org/10.2174/1566524019666190308115624},
  volume       = {19},
  year         = {2019},
}