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Controlled fabrication of gelatin nanoparticles as drug carriers

Jahanshahi, M. ; Sanati, Mohammad Hossein ; Minuchehr, Z. ; Hajizadeh, S. LU orcid and Babaei, Z. (2007) NANOTECHNOLOGY AND ITS APPLICATIONS: First Sharjah International Conference on Nanotechnology and Its Applications In AIP Conference Proceedings 929. p.228-232
Abstract

In recent years, significant effort has been devoted to develop nanotechnology for drug delivery since it offers a suitable means of delivering small molecular weight drugs, as well as macromolecules such as proteins, peptides or genes by either localized or targeted delivery to the tissue of interest. Nanotechnology focuses on formulating therapeutic agents in biocompatible nanocomposites such as nanoparticles, nanocapsules, micellar systems, and conjugates. Protein nanoparticles (BSA HAS and gelatin) generally vary in size from 50-300 nm and they hold certain advantages such as greater stability during storage, stability in vivo, non-toxicity, non-antigen and ease to scale up during manufacture over the other drug delivery systems.... (More)

In recent years, significant effort has been devoted to develop nanotechnology for drug delivery since it offers a suitable means of delivering small molecular weight drugs, as well as macromolecules such as proteins, peptides or genes by either localized or targeted delivery to the tissue of interest. Nanotechnology focuses on formulating therapeutic agents in biocompatible nanocomposites such as nanoparticles, nanocapsules, micellar systems, and conjugates. Protein nanoparticles (BSA HAS and gelatin) generally vary in size from 50-300 nm and they hold certain advantages such as greater stability during storage, stability in vivo, non-toxicity, non-antigen and ease to scale up during manufacture over the other drug delivery systems. The primary structure of gelatin offers many possibilities for chemical modification and covalent drug attachment. Here nanoparticles of gelatin type A were prepared by a two-step desolvation method as a colloidal drug delivery system and the essential parameters in fabrication were considered. Gelatin was dissolved in 25 mL distilled water under room temperature range. Then acetone was added to the gelatin solution as a desolvating agent to precipitate the high molecular weight (HMW) gelatin. The supernatant was discarded and the HMW gelatin re-dissolved by adding 25 mL distilled water and stirring at 600 rpm. Acetone were added drop-wise to form nanoparticles. At the end of the process, glutaraldehyde solution was used for preparing nanoparticles as a cross-linking agent, and stirred for 12h at 600 rpm. For purification stage we use centrifuge with 600rpm for 3 times. The objective of the present study is consideration of some factors such as temperature, gelatin concentration, agitation speed and the amount of acetone and their effects on size and distribution of nanoparticles. Among the all conditions, 60°C, 50 mg/ml gelatin concentration, 75 ml acetone had the best result and the nanoparticle size was under 170 nm. The effect of these factors for synthesis of gelatine nanoparticle is strongly discussed.

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Please use this url to cite or link to this publication:
author
; ; ; and
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
keywords
Drug delivery, Fabrication, Gelatin nanoparticles, Optimization
host publication
NANOTECHNOLOGY AND ITS APPLICATIONS : First Sharjah International Conference on Nanotechnology and Its Applications - First Sharjah International Conference on Nanotechnology and Its Applications
series title
AIP Conference Proceedings
volume
929
pages
5 pages
conference name
NANOTECHNOLOGY AND ITS APPLICATIONS: First Sharjah International Conference on Nanotechnology and Its Applications
conference location
Sharjah, United Arab Emirates
conference dates
2007-04-10 - 2007-04-12
external identifiers
  • scopus:36749013545
ISSN
1551-7616
0094-243X
ISBN
0735404399
9780735404397
DOI
10.1063/1.2776720
language
English
LU publication?
no
id
5e98baa6-d11f-4d52-b9f5-b0c2ea64c336
date added to LUP
2023-08-24 08:34:25
date last changed
2024-03-23 00:07:58
@inproceedings{5e98baa6-d11f-4d52-b9f5-b0c2ea64c336,
  abstract     = {{<p>In recent years, significant effort has been devoted to develop nanotechnology for drug delivery since it offers a suitable means of delivering small molecular weight drugs, as well as macromolecules such as proteins, peptides or genes by either localized or targeted delivery to the tissue of interest. Nanotechnology focuses on formulating therapeutic agents in biocompatible nanocomposites such as nanoparticles, nanocapsules, micellar systems, and conjugates. Protein nanoparticles (BSA HAS and gelatin) generally vary in size from 50-300 nm and they hold certain advantages such as greater stability during storage, stability in vivo, non-toxicity, non-antigen and ease to scale up during manufacture over the other drug delivery systems. The primary structure of gelatin offers many possibilities for chemical modification and covalent drug attachment. Here nanoparticles of gelatin type A were prepared by a two-step desolvation method as a colloidal drug delivery system and the essential parameters in fabrication were considered. Gelatin was dissolved in 25 mL distilled water under room temperature range. Then acetone was added to the gelatin solution as a desolvating agent to precipitate the high molecular weight (HMW) gelatin. The supernatant was discarded and the HMW gelatin re-dissolved by adding 25 mL distilled water and stirring at 600 rpm. Acetone were added drop-wise to form nanoparticles. At the end of the process, glutaraldehyde solution was used for preparing nanoparticles as a cross-linking agent, and stirred for 12h at 600 rpm. For purification stage we use centrifuge with 600rpm for 3 times. The objective of the present study is consideration of some factors such as temperature, gelatin concentration, agitation speed and the amount of acetone and their effects on size and distribution of nanoparticles. Among the all conditions, 60°C, 50 mg/ml gelatin concentration, 75 ml acetone had the best result and the nanoparticle size was under 170 nm. The effect of these factors for synthesis of gelatine nanoparticle is strongly discussed.</p>}},
  author       = {{Jahanshahi, M. and Sanati, Mohammad Hossein and Minuchehr, Z. and Hajizadeh, S. and Babaei, Z.}},
  booktitle    = {{NANOTECHNOLOGY AND ITS APPLICATIONS : First Sharjah International Conference on Nanotechnology and Its Applications}},
  isbn         = {{0735404399}},
  issn         = {{1551-7616}},
  keywords     = {{Drug delivery; Fabrication; Gelatin nanoparticles; Optimization}},
  language     = {{eng}},
  pages        = {{228--232}},
  series       = {{AIP Conference Proceedings}},
  title        = {{Controlled fabrication of gelatin nanoparticles as drug carriers}},
  url          = {{http://dx.doi.org/10.1063/1.2776720}},
  doi          = {{10.1063/1.2776720}},
  volume       = {{929}},
  year         = {{2007}},
}