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Amount of stroma is associated with mammographic density and stromal expression of oestrogen receptor in normal breast tissues

Gabrielson, Marike; Chiesa, Flaminia; Paulsson, Janna; Strell, Carina; Behmer, Catharina; Rönnow, Katarina; Czene, Kamila; Östman, Arne and Hall, Per (2016) In Breast Cancer Research and Treatment 158(2). p.253-261
Abstract

Following female sex and age, mammographic density is considered one of the strongest risk factors for breast cancer. Despite the association between mammographic density and breast cancer risk, little is known about the underlying histology and biological basis of breast density. To better understand the mechanisms behind mammographic density we assessed morphology, proliferation and hormone receptor status in relation to mammographic density in breast tissues from healthy women. Tissues were obtained from 2012–2013 by ultrasound-guided core needle biopsy from 160 women as part of the Karma (Karolinska mammography project for risk prediction for breast cancer) project. Mammograms were collected through routine mammography screening and... (More)

Following female sex and age, mammographic density is considered one of the strongest risk factors for breast cancer. Despite the association between mammographic density and breast cancer risk, little is known about the underlying histology and biological basis of breast density. To better understand the mechanisms behind mammographic density we assessed morphology, proliferation and hormone receptor status in relation to mammographic density in breast tissues from healthy women. Tissues were obtained from 2012–2013 by ultrasound-guided core needle biopsy from 160 women as part of the Karma (Karolinska mammography project for risk prediction for breast cancer) project. Mammograms were collected through routine mammography screening and mammographic density was calculated using STRATUS. The histological composition, epithelial and stromal proliferation status and hormone receptor status were assessed through immunohistochemical staining. Higher mammographic density was significantly associated with a greater proportion of stromal and epithelial tissue and a lower proportion of adipose tissue. Epithelial expression levels of Ki-67, oestrogen receptor (ER) and progesterone receptor (PR) were not associated with mammographic density. Epithelial Ki-67 was associated with a greater proportion of epithelial tissue, and epithelial PR was associated with a greater proportion of stromal and a lower proportion of adipose tissue. Epithelial ER was not associated with any tissues. In contrast, expression of ER in the stroma was significantly associated with a greater proportion of stroma, and negatively associated with the amount of adipose tissue. High mammographic density is associated with higher amount of stroma and epithelium and less amount of fat, but is not associated with a change in epithelial proliferation or receptor status. Increased expressions of both epithelial PR and stromal ER are associated with a greater proportion of stroma, suggesting hormonal involvement in regulating breast tissue composition.

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author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Breast cancer, Breast tissue, Histology, Hormone receptor status, Mammographic density, Stromal breast tissue
in
Breast Cancer Research and Treatment
volume
158
issue
2
pages
9 pages
publisher
Springer
external identifiers
  • Scopus:84976286247
ISSN
0167-6806
DOI
10.1007/s10549-016-3877-x
language
English
LU publication?
no
id
5ec6f519-e6b9-4b5c-839c-bf2868e69cbd
date added to LUP
2016-07-21 11:39:14
date last changed
2017-01-01 08:30:56
@article{5ec6f519-e6b9-4b5c-839c-bf2868e69cbd,
  abstract     = {<p>Following female sex and age, mammographic density is considered one of the strongest risk factors for breast cancer. Despite the association between mammographic density and breast cancer risk, little is known about the underlying histology and biological basis of breast density. To better understand the mechanisms behind mammographic density we assessed morphology, proliferation and hormone receptor status in relation to mammographic density in breast tissues from healthy women. Tissues were obtained from 2012–2013 by ultrasound-guided core needle biopsy from 160 women as part of the Karma (Karolinska mammography project for risk prediction for breast cancer) project. Mammograms were collected through routine mammography screening and mammographic density was calculated using STRATUS. The histological composition, epithelial and stromal proliferation status and hormone receptor status were assessed through immunohistochemical staining. Higher mammographic density was significantly associated with a greater proportion of stromal and epithelial tissue and a lower proportion of adipose tissue. Epithelial expression levels of Ki-67, oestrogen receptor (ER) and progesterone receptor (PR) were not associated with mammographic density. Epithelial Ki-67 was associated with a greater proportion of epithelial tissue, and epithelial PR was associated with a greater proportion of stromal and a lower proportion of adipose tissue. Epithelial ER was not associated with any tissues. In contrast, expression of ER in the stroma was significantly associated with a greater proportion of stroma, and negatively associated with the amount of adipose tissue. High mammographic density is associated with higher amount of stroma and epithelium and less amount of fat, but is not associated with a change in epithelial proliferation or receptor status. Increased expressions of both epithelial PR and stromal ER are associated with a greater proportion of stroma, suggesting hormonal involvement in regulating breast tissue composition.</p>},
  author       = {Gabrielson, Marike and Chiesa, Flaminia and Paulsson, Janna and Strell, Carina and Behmer, Catharina and Rönnow, Katarina and Czene, Kamila and Östman, Arne and Hall, Per},
  issn         = {0167-6806},
  keyword      = {Breast cancer,Breast tissue,Histology,Hormone receptor status,Mammographic density,Stromal breast tissue},
  language     = {eng},
  month        = {07},
  number       = {2},
  pages        = {253--261},
  publisher    = {Springer},
  series       = {Breast Cancer Research and Treatment},
  title        = {Amount of stroma is associated with mammographic density and stromal expression of oestrogen receptor in normal breast tissues},
  url          = {http://dx.doi.org/10.1007/s10549-016-3877-x},
  volume       = {158},
  year         = {2016},
}