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Biocompatible scaffolds based on natural polymers for regenerative medicine

Akilbekova, Dana; Shaimerdenova, Madina; Adilov, Salimgerey and Berillo, Dmitriy LU (2018) In International Journal of Biological Macromolecules 114. p.324-333
Abstract

The chitosan and gelatine are commonly used biopolymers for the tissue engineering applications. In the previous methods for the cryogels synthesis, multistep preparation methods using toxic cross-linking agents such as glutaraldehyde are reported. Here, we present a two-step preparation method of gelatin macroporous cryogels and one-step preparation method of chitosan or gelatin cryogels. The physico-chemical properties of obtained scaffolds were characterized using FTIR, zeta potential, SEM and laser confocal microscopy. Non-toxic and biodegradable cross-linking agents such as oxidized dextran and 1,1,3,3-tetramethoxypropane are utilized. The one-step chitosan cryogels had degradation degree ~2 times higher compared to the cryogels... (More)

The chitosan and gelatine are commonly used biopolymers for the tissue engineering applications. In the previous methods for the cryogels synthesis, multistep preparation methods using toxic cross-linking agents such as glutaraldehyde are reported. Here, we present a two-step preparation method of gelatin macroporous cryogels and one-step preparation method of chitosan or gelatin cryogels. The physico-chemical properties of obtained scaffolds were characterized using FTIR, zeta potential, SEM and laser confocal microscopy. Non-toxic and biodegradable cross-linking agents such as oxidized dextran and 1,1,3,3-tetramethoxypropane are utilized. The one-step chitosan cryogels had degradation degree ~2 times higher compared to the cryogels prepared with a two-step method i.e. reduced by borohydride. Scaffolds cross-linked by glutaraldehyde had about 40% viability, whereas nine various compositions of cryogels showed significantly higher viability (~80%) of fibroblast cells in vitro. The cryogels were obtained without using the harmful compounds and therefore can be used straightforward as biocompatible and biodegradable scaffolds for the cell culturing purposes and other biomedical applications.

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organization
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type
Contribution to journal
publication status
published
subject
keywords
1,1,3,3-tetramethoxypropane, Chitosan, Cryogels, Natural polymers
in
International Journal of Biological Macromolecules
volume
114
pages
10 pages
publisher
Elsevier
external identifiers
  • scopus:85044583948
ISSN
0141-8130
DOI
10.1016/j.ijbiomac.2018.03.116
language
English
LU publication?
yes
id
5f3c4398-9734-493b-ac78-7cb6ea047109
date added to LUP
2018-04-10 09:52:54
date last changed
2019-03-19 03:52:35
@article{5f3c4398-9734-493b-ac78-7cb6ea047109,
  abstract     = {<p>The chitosan and gelatine are commonly used biopolymers for the tissue engineering applications. In the previous methods for the cryogels synthesis, multistep preparation methods using toxic cross-linking agents such as glutaraldehyde are reported. Here, we present a two-step preparation method of gelatin macroporous cryogels and one-step preparation method of chitosan or gelatin cryogels. The physico-chemical properties of obtained scaffolds were characterized using FTIR, zeta potential, SEM and laser confocal microscopy. Non-toxic and biodegradable cross-linking agents such as oxidized dextran and 1,1,3,3-tetramethoxypropane are utilized. The one-step chitosan cryogels had degradation degree ~2 times higher compared to the cryogels prepared with a two-step method i.e. reduced by borohydride. Scaffolds cross-linked by glutaraldehyde had about 40% viability, whereas nine various compositions of cryogels showed significantly higher viability (~80%) of fibroblast cells in vitro. The cryogels were obtained without using the harmful compounds and therefore can be used straightforward as biocompatible and biodegradable scaffolds for the cell culturing purposes and other biomedical applications.</p>},
  author       = {Akilbekova, Dana and Shaimerdenova, Madina and Adilov, Salimgerey and Berillo, Dmitriy},
  issn         = {0141-8130},
  keyword      = {1,1,3,3-tetramethoxypropane,Chitosan,Cryogels,Natural polymers},
  language     = {eng},
  month        = {07},
  pages        = {324--333},
  publisher    = {Elsevier},
  series       = {International Journal of Biological Macromolecules},
  title        = {Biocompatible scaffolds based on natural polymers for regenerative medicine},
  url          = {http://dx.doi.org/10.1016/j.ijbiomac.2018.03.116},
  volume       = {114},
  year         = {2018},
}