Is a bacterial biofilm in the lacrimal sac the cause of chronic refractory dacryocystitis?–A pilot study
Sonesson, Andreas LU ; Baumgarten, Maria LU ; Bhongir, Ravi LU
and Engelsberg, Karl
LU
(2023)
In Orbit (London)
- Abstract
Purpose: A pilot study to identify bacterial biofilm in the lacrimal sacs of patients with chronic dacryocystitis, and in patients with epiphora but without discharge, using scanning electron microscopy. Methods: Five patients: two with nasolacrimal duct obstruction without dacryocystitis, and three with dacryocystitis refractory to antibiotics, underwent external dacryocystorhinostomy. One control patient without infection was included. Bacterial cultures were obtained from the lumen of the lacrimal sac to analyze possible bacterial growth, including antibiotic resistance. Biopsies were taken from all lacrimal sacs and prepared for light and scanning electron microscopy. Results: Scanning electron microscopy of all the lacrimal sac... (More)
Purpose: A pilot study to identify bacterial biofilm in the lacrimal sacs of patients with chronic dacryocystitis, and in patients with epiphora but without discharge, using scanning electron microscopy. Methods: Five patients: two with nasolacrimal duct obstruction without dacryocystitis, and three with dacryocystitis refractory to antibiotics, underwent external dacryocystorhinostomy. One control patient without infection was included. Bacterial cultures were obtained from the lumen of the lacrimal sac to analyze possible bacterial growth, including antibiotic resistance. Biopsies were taken from all lacrimal sacs and prepared for light and scanning electron microscopy. Results: Scanning electron microscopy of all the lacrimal sac samples revealed structures consistent with bacterial communities and adjacent extracellular material, indicating biofilm formation. This was most prominent in one of the patients with chronic dacryocystitis. Bacteria were found not only on the luminal surface of the sac, but also within the tissue of the sac. Bacterial growth was identified in samples from two patients with chronic dacryocystitis, whereas samples from the other three patients showed no bacterial growth. Conclusion: Lack of patency of the lacrimal duct predisposes to bacterial growth, even in patients with no clinically confirmed infection of the lacrimal sac. The finding of a biofilm in patients with chronic dacryocystitis explains the lack of efficiency of antibiotic treatment at the concentrations used in clinical practice.
(Less)
- author
- Sonesson, Andreas
LU
; Baumgarten, Maria
LU
; Bhongir, Ravi
LU
and Engelsberg, Karl
LU
- organization
- publishing date
- 2023
- type
- Contribution to journal
- publication status
- in press
- subject
- keywords
- biofilm, Dacryocystitis, electron microscopy
- in
- Orbit (London)
- publisher
- Informa Healthcare
- external identifiers
-
- pmid:37885304
- scopus:85174946264
- ISSN
- 0167-6830
- DOI
- 10.1080/01676830.2023.2269580
- language
- English
- LU publication?
- yes
- additional info
- Publisher Copyright: © 2023 The Author(s). Published with license by Taylor & Francis Group, LLC.
- id
- 5f73ab92-3883-47bd-8075-44c11aa54878
- date added to LUP
- 2023-12-13 13:36:22
- date last changed
- 2024-04-12 04:24:21
@article{5f73ab92-3883-47bd-8075-44c11aa54878,
abstract = {{<p>Purpose: A pilot study to identify bacterial biofilm in the lacrimal sacs of patients with chronic dacryocystitis, and in patients with epiphora but without discharge, using scanning electron microscopy. Methods: Five patients: two with nasolacrimal duct obstruction without dacryocystitis, and three with dacryocystitis refractory to antibiotics, underwent external dacryocystorhinostomy. One control patient without infection was included. Bacterial cultures were obtained from the lumen of the lacrimal sac to analyze possible bacterial growth, including antibiotic resistance. Biopsies were taken from all lacrimal sacs and prepared for light and scanning electron microscopy. Results: Scanning electron microscopy of all the lacrimal sac samples revealed structures consistent with bacterial communities and adjacent extracellular material, indicating biofilm formation. This was most prominent in one of the patients with chronic dacryocystitis. Bacteria were found not only on the luminal surface of the sac, but also within the tissue of the sac. Bacterial growth was identified in samples from two patients with chronic dacryocystitis, whereas samples from the other three patients showed no bacterial growth. Conclusion: Lack of patency of the lacrimal duct predisposes to bacterial growth, even in patients with no clinically confirmed infection of the lacrimal sac. The finding of a biofilm in patients with chronic dacryocystitis explains the lack of efficiency of antibiotic treatment at the concentrations used in clinical practice.</p>}},
author = {{Sonesson, Andreas and Baumgarten, Maria and Bhongir, Ravi and Engelsberg, Karl}},
issn = {{0167-6830}},
keywords = {{biofilm; Dacryocystitis; electron microscopy}},
language = {{eng}},
publisher = {{Informa Healthcare}},
series = {{Orbit (London)}},
title = {{Is a bacterial biofilm in the lacrimal sac the cause of chronic refractory dacryocystitis?–A pilot study}},
url = {{http://dx.doi.org/10.1080/01676830.2023.2269580}},
doi = {{10.1080/01676830.2023.2269580}},
year = {{2023}},
}