Shikimic acid production by a modified strain of E-coli (W3110.shik1) under phosphate-limited and carbon-limited conditions
(2005) In Biotechnology and Bioengineering 92(5). p.541-552- Abstract
- Shikimic acid is one of several industrially interesting chiral starting materials formed in the aromatic amino acid pathway of plants and microorganisms. In this study, the physiology of a shikimic acid producing strain of Escherichia coli (derived from W31 10) deleted in aroL (shikimic acid kinase II gene), was compared to that of a corresponding control strain W31 10) under carbonand phosphate-limited conditions. For the shikimic acid producing strain (referred to as W31 10.shik1), phosphate limitation resulted in a higher yield of shikimic acid (0.059 +/- 0.012 vs. 0.024 +/- 0.005 c-mol/c-mol) and a lower yield of by-products from the shikimate pathway, when compared to carbon-limited condition. The yield of the by-product... (More)
- Shikimic acid is one of several industrially interesting chiral starting materials formed in the aromatic amino acid pathway of plants and microorganisms. In this study, the physiology of a shikimic acid producing strain of Escherichia coli (derived from W31 10) deleted in aroL (shikimic acid kinase II gene), was compared to that of a corresponding control strain W31 10) under carbonand phosphate-limited conditions. For the shikimic acid producing strain (referred to as W31 10.shik1), phosphate limitation resulted in a higher yield of shikimic acid (0.059 +/- 0.012 vs. 0.024 +/- 0.005 c-mol/c-mol) and a lower yield of by-products from the shikimate pathway, when compared to carbon-limited condition. The yield of the by-product 3-clehydroshikimic acid (DHS) decreased from 0.076 +/- 0.028 to 0.022 +/- 0.001 c-mol/c-mol. Several other by-products were only detected under carbon-limited conditions. The latter group included 3-dehydroquinic acid (0.021 +/- 0.021 c-mol/c-mol), quinic acid (0.012 +/- 0.005 c-mol/c-mol), and gallic acid (0.002 +/- 0.001 c-mol/c-mol). For both strains, more acetate was produced under phosphate than the carbon-limited case. Considerable cell lysis was found for both strains but was higher for W3110.shikl ' and increased for both strains under phosphate limitation. The advantages of the latter condition in terms of an increased shikimic acid yield was thus counteracted by an increased cell lysis, which may make downstream processing more difficult. (c) 2005 Wiley Periodicals, Inc. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/151582
- author
- Johansson, Louise LU ; Lindskog, A ; Silfversparre, G ; Cimander, C ; Nielsen, K F and Lidén, Gunnar LU
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Biotechnology and Bioengineering
- volume
- 92
- issue
- 5
- pages
- 541 - 552
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000233743600003
- pmid:16240440
- scopus:29544436147
- pmid:16240440
- ISSN
- 1097-0290
- DOI
- 10.1002/bit.20546
- language
- English
- LU publication?
- yes
- id
- 5f9a607f-1564-40cd-ad56-156bd3ba8a77 (old id 151582)
- date added to LUP
- 2016-04-01 12:37:32
- date last changed
- 2023-11-12 07:37:35
@article{5f9a607f-1564-40cd-ad56-156bd3ba8a77,
abstract = {{Shikimic acid is one of several industrially interesting chiral starting materials formed in the aromatic amino acid pathway of plants and microorganisms. In this study, the physiology of a shikimic acid producing strain of Escherichia coli (derived from W31 10) deleted in aroL (shikimic acid kinase II gene), was compared to that of a corresponding control strain W31 10) under carbonand phosphate-limited conditions. For the shikimic acid producing strain (referred to as W31 10.shik1), phosphate limitation resulted in a higher yield of shikimic acid (0.059 +/- 0.012 vs. 0.024 +/- 0.005 c-mol/c-mol) and a lower yield of by-products from the shikimate pathway, when compared to carbon-limited condition. The yield of the by-product 3-clehydroshikimic acid (DHS) decreased from 0.076 +/- 0.028 to 0.022 +/- 0.001 c-mol/c-mol. Several other by-products were only detected under carbon-limited conditions. The latter group included 3-dehydroquinic acid (0.021 +/- 0.021 c-mol/c-mol), quinic acid (0.012 +/- 0.005 c-mol/c-mol), and gallic acid (0.002 +/- 0.001 c-mol/c-mol). For both strains, more acetate was produced under phosphate than the carbon-limited case. Considerable cell lysis was found for both strains but was higher for W3110.shikl ' and increased for both strains under phosphate limitation. The advantages of the latter condition in terms of an increased shikimic acid yield was thus counteracted by an increased cell lysis, which may make downstream processing more difficult. (c) 2005 Wiley Periodicals, Inc.}},
author = {{Johansson, Louise and Lindskog, A and Silfversparre, G and Cimander, C and Nielsen, K F and Lidén, Gunnar}},
issn = {{1097-0290}},
language = {{eng}},
number = {{5}},
pages = {{541--552}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Biotechnology and Bioengineering}},
title = {{Shikimic acid production by a modified strain of E-coli (W3110.shik1) under phosphate-limited and carbon-limited conditions}},
url = {{http://dx.doi.org/10.1002/bit.20546}},
doi = {{10.1002/bit.20546}},
volume = {{92}},
year = {{2005}},
}