Cytogenetic features of multiple myeloma: impact of gender, age, disease phase, culture time, and cytokine stimulation
(2002) In European Journal of Haematology 68(6). p.345-353- Abstract
- Relatively little is known about the cytogenetic features of multiple myeloma (MM) when compared to other hematologic malignancies. The reasons for this are most likely manifold, and include a low mitotic index of the malignant cells and the presence of cytogenetically cryptic abnormalities as well as of complex karyotypes with poor chromosome morphology. In the present study, we have investigated whether various culture conditions may influence the yield of abnormal metaphases in MM and, in the related plasma cell dyscrasias, monoclonal gammopathy of undetermined significance (MGUS) and plasmacytomas (PC). In addition, the possible impact of age, gender, and disease phase on the cytogenetic features has been analyzed. A total of 95... (More)
- Relatively little is known about the cytogenetic features of multiple myeloma (MM) when compared to other hematologic malignancies. The reasons for this are most likely manifold, and include a low mitotic index of the malignant cells and the presence of cytogenetically cryptic abnormalities as well as of complex karyotypes with poor chromosome morphology. In the present study, we have investigated whether various culture conditions may influence the yield of abnormal metaphases in MM and, in the related plasma cell dyscrasias, monoclonal gammopathy of undetermined significance (MGUS) and plasmacytomas (PC). In addition, the possible impact of age, gender, and disease phase on the cytogenetic features has been analyzed. A total of 95 samples from 74 cases (68 MM, three PC, and three MGUS patients) were obtained for cytogenetic analysis. The samples were cultured either in conventional medium or in medium containing IL-6 and GM-CSF, and the culture times varied from 24 to 120 h. In total, 186 cultures were analyzed. Metaphase fluorescence in situ hybridization analysis using probes specific for 14q32, i.e. IGH rearrangements, could be performed in 57 of the 74 cases, and revealed 14q32 aberrations in 10 cases not seen by conventional G-banding. Abnormal karyotypes were detected in 77 (41%) of the 186 cultures, 46 (48%) of the 95 samples, and in 41 (55%) of the 74 patients, revealing a total of 20 chromosomal aberrations previously not reported in plasma cell dyscrasias. We found no evidence that gender, age, disease phase, culture time, or cytokine stimulation significantly influences the karyotypic features of MM. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/329235
- author
- Nilsson, Therese LU ; Lenhoff, S ; Turesson, Ingemar LU ; Rylander, Lars LU ; Mitelman, Felix LU ; Westin, J ; Höglund, Mattias LU and Johansson, Bertil LU
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- disease phase, gender, age, multiple myeloma, cytogenetics, culture, conditions
- in
- European Journal of Haematology
- volume
- 68
- issue
- 6
- pages
- 345 - 353
- publisher
- Wiley-Blackwell
- external identifiers
-
- pmid:12225392
- wos:000177965100004
- scopus:0036620705
- ISSN
- 1600-0609
- DOI
- 10.1034/j.1600-0609.2002.00724.x
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Division of Occupational and Environmental Medicine (013078001), Emergency medicine/Medicine/Surgery (013240200), Division of Clinical Genetics (013022003)
- id
- 5faf56f5-c3ac-429a-ae81-da0a7f3f7749 (old id 329235)
- date added to LUP
- 2016-04-01 11:47:49
- date last changed
- 2022-04-05 05:11:58
@article{5faf56f5-c3ac-429a-ae81-da0a7f3f7749, abstract = {{Relatively little is known about the cytogenetic features of multiple myeloma (MM) when compared to other hematologic malignancies. The reasons for this are most likely manifold, and include a low mitotic index of the malignant cells and the presence of cytogenetically cryptic abnormalities as well as of complex karyotypes with poor chromosome morphology. In the present study, we have investigated whether various culture conditions may influence the yield of abnormal metaphases in MM and, in the related plasma cell dyscrasias, monoclonal gammopathy of undetermined significance (MGUS) and plasmacytomas (PC). In addition, the possible impact of age, gender, and disease phase on the cytogenetic features has been analyzed. A total of 95 samples from 74 cases (68 MM, three PC, and three MGUS patients) were obtained for cytogenetic analysis. The samples were cultured either in conventional medium or in medium containing IL-6 and GM-CSF, and the culture times varied from 24 to 120 h. In total, 186 cultures were analyzed. Metaphase fluorescence in situ hybridization analysis using probes specific for 14q32, i.e. IGH rearrangements, could be performed in 57 of the 74 cases, and revealed 14q32 aberrations in 10 cases not seen by conventional G-banding. Abnormal karyotypes were detected in 77 (41%) of the 186 cultures, 46 (48%) of the 95 samples, and in 41 (55%) of the 74 patients, revealing a total of 20 chromosomal aberrations previously not reported in plasma cell dyscrasias. We found no evidence that gender, age, disease phase, culture time, or cytokine stimulation significantly influences the karyotypic features of MM.}}, author = {{Nilsson, Therese and Lenhoff, S and Turesson, Ingemar and Rylander, Lars and Mitelman, Felix and Westin, J and Höglund, Mattias and Johansson, Bertil}}, issn = {{1600-0609}}, keywords = {{disease phase; gender; age; multiple myeloma; cytogenetics; culture; conditions}}, language = {{eng}}, number = {{6}}, pages = {{345--353}}, publisher = {{Wiley-Blackwell}}, series = {{European Journal of Haematology}}, title = {{Cytogenetic features of multiple myeloma: impact of gender, age, disease phase, culture time, and cytokine stimulation}}, url = {{http://dx.doi.org/10.1034/j.1600-0609.2002.00724.x}}, doi = {{10.1034/j.1600-0609.2002.00724.x}}, volume = {{68}}, year = {{2002}}, }