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Transmembrane topology of FRO2, a ferric chelate reductase from Arabidopsis thaliana

Schagerlöf, Ulrika LU ; Wilson, Greer; Hebert, Hans LU ; Al-Karadaghi, Salam LU and Hägerhäll, Cecilia LU (2006) In Plant Molecular Biology 62(1-2). p.215-221
Abstract
Iron uptake in Arabidopsis thaliana is mediated by ferric chelate reductase FRO2, a transmembrane protein belonging to the flavocytochrome b family. There is no high resolution structural information available for any member of this family. We have determined the transmembrane topology of FRO2 experimentally using the alkaline phosphatase fusion method. The resulting topology is different from that obtained by theoretical predictions and contains 8 transmembrane helices, 4 of which build up the highly conserved core of the protein. This core is present in the entire flavocytochrome b family. The large water soluble domain of FRO2, which contains NADPH, FAD and oxidoreductase sequence motifs, was located on the inside of the membrane.
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
gp91phox, FRO2, ferric-chelate reductases, oxidases, superoxide generating NADPH, iron transport, alkaline phosphatase
in
Plant Molecular Biology
volume
62
issue
1-2
pages
215 - 221
publisher
Kluwer
external identifiers
  • wos:000240399300016
  • scopus:33748555572
ISSN
1573-5028
DOI
10.1007/s11103-006-9015-0
language
English
LU publication?
yes
id
5fd09e5a-b270-4748-ae6b-b49f8769db33 (old id 393390)
date added to LUP
2007-10-17 19:54:55
date last changed
2019-06-19 01:41:09
@article{5fd09e5a-b270-4748-ae6b-b49f8769db33,
  abstract     = {Iron uptake in Arabidopsis thaliana is mediated by ferric chelate reductase FRO2, a transmembrane protein belonging to the flavocytochrome b family. There is no high resolution structural information available for any member of this family. We have determined the transmembrane topology of FRO2 experimentally using the alkaline phosphatase fusion method. The resulting topology is different from that obtained by theoretical predictions and contains 8 transmembrane helices, 4 of which build up the highly conserved core of the protein. This core is present in the entire flavocytochrome b family. The large water soluble domain of FRO2, which contains NADPH, FAD and oxidoreductase sequence motifs, was located on the inside of the membrane.},
  author       = {Schagerlöf, Ulrika and Wilson, Greer and Hebert, Hans and Al-Karadaghi, Salam and Hägerhäll, Cecilia},
  issn         = {1573-5028},
  keyword      = {gp91phox,FRO2,ferric-chelate reductases,oxidases,superoxide generating NADPH,iron transport,alkaline phosphatase},
  language     = {eng},
  number       = {1-2},
  pages        = {215--221},
  publisher    = {Kluwer},
  series       = {Plant Molecular Biology},
  title        = {Transmembrane topology of FRO2, a ferric chelate reductase from Arabidopsis thaliana},
  url          = {http://dx.doi.org/10.1007/s11103-006-9015-0},
  volume       = {62},
  year         = {2006},
}