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Comparative analysis of mitochondrion-related organelles in anaerobic amoebozoans

Záhonová, Kristína ; Füssy, Zoltán ; Stairs, Courtney W LU orcid ; Leger, Michelle M ; Tachezy, Jan ; Čepička, Ivan ; Roger, Andrew J and Hampl, Vladimír (2023) In Microbial Genomics 9(11).
Abstract

Archamoebae comprises free-living or endobiotic amoebiform protists that inhabit anaerobic or microaerophilic environments and possess mitochondrion-related organelles (MROs) adapted to function anaerobically. We compared
in silico reconstructed MRO proteomes of eight species (six genera) and found that the common ancestor of Archamoebae possessed very few typical components of the protein translocation machinery, electron transport chain and tricarboxylic acid cycle. On the other hand, it contained a sulphate activation pathway and bacterial iron-sulphur (Fe-S) assembly system of MIS-type. The metabolic capacity of the MROs, however, varies markedly within this clade. The glycine cleavage system is widely conserved among... (More)

Archamoebae comprises free-living or endobiotic amoebiform protists that inhabit anaerobic or microaerophilic environments and possess mitochondrion-related organelles (MROs) adapted to function anaerobically. We compared
in silico reconstructed MRO proteomes of eight species (six genera) and found that the common ancestor of Archamoebae possessed very few typical components of the protein translocation machinery, electron transport chain and tricarboxylic acid cycle. On the other hand, it contained a sulphate activation pathway and bacterial iron-sulphur (Fe-S) assembly system of MIS-type. The metabolic capacity of the MROs, however, varies markedly within this clade. The glycine cleavage system is widely conserved among Archamoebae, except in
Entamoeba, probably owing to its role in catabolic function or one-carbon metabolism. MRO-based pyruvate metabolism was dispensed within subgroups Entamoebidae and Rhizomastixidae, whereas sulphate activation could have been lost in isolated cases of
Rhizomastix libera,
Mastigamoeba abducta and
Endolimax sp. The MIS (Fe-S) assembly system was duplicated in the common ancestor of Mastigamoebidae and Pelomyxidae, and one of the copies took over Fe-S assembly in their MRO. In Entamoebidae and Rhizomastixidae, we hypothesize that Fe-S cluster assembly in both compartments may be facilitated by dual localization of the single system. We could not find evidence for changes in metabolic functions of the MRO in response to changes in habitat; it appears that such environmental drivers do not strongly affect MRO reduction in this group of eukaryotes.

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author
; ; ; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
keywords
Anaerobiosis, Mitochondria/genetics, Eukaryota, Iron, Sulfates
in
Microbial Genomics
volume
9
issue
11
pages
14 pages
publisher
Microbiology Society
external identifiers
  • scopus:85177866881
  • pmid:37994879
ISSN
2057-5858
DOI
10.1099/mgen.0.001143
language
English
LU publication?
no
id
60314d19-c622-4e25-abec-179f64bc5768
date added to LUP
2023-11-26 17:17:53
date last changed
2024-04-22 02:10:30
@article{60314d19-c622-4e25-abec-179f64bc5768,
  abstract     = {{<p>Archamoebae comprises free-living or endobiotic amoebiform protists that inhabit anaerobic or microaerophilic environments and possess mitochondrion-related organelles (MROs) adapted to function anaerobically. We compared <br>
 in silico reconstructed MRO proteomes of eight species (six genera) and found that the common ancestor of Archamoebae possessed very few typical components of the protein translocation machinery, electron transport chain and tricarboxylic acid cycle. On the other hand, it contained a sulphate activation pathway and bacterial iron-sulphur (Fe-S) assembly system of MIS-type. The metabolic capacity of the MROs, however, varies markedly within this clade. The glycine cleavage system is widely conserved among Archamoebae, except in<br>
 Entamoeba, probably owing to its role in catabolic function or one-carbon metabolism. MRO-based pyruvate metabolism was dispensed within subgroups Entamoebidae and Rhizomastixidae, whereas sulphate activation could have been lost in isolated cases of <br>
 Rhizomastix libera, <br>
 Mastigamoeba abducta and <br>
 Endolimax sp. The MIS (Fe-S) assembly system was duplicated in the common ancestor of Mastigamoebidae and Pelomyxidae, and one of the copies took over Fe-S assembly in their MRO. In Entamoebidae and Rhizomastixidae, we hypothesize that Fe-S cluster assembly in both compartments may be facilitated by dual localization of the single system. We could not find evidence for changes in metabolic functions of the MRO in response to changes in habitat; it appears that such environmental drivers do not strongly affect MRO reduction in this group of eukaryotes.<br>
 </p>}},
  author       = {{Záhonová, Kristína and Füssy, Zoltán and Stairs, Courtney W and Leger, Michelle M and Tachezy, Jan and Čepička, Ivan and Roger, Andrew J and Hampl, Vladimír}},
  issn         = {{2057-5858}},
  keywords     = {{Anaerobiosis; Mitochondria/genetics; Eukaryota; Iron; Sulfates}},
  language     = {{eng}},
  number       = {{11}},
  publisher    = {{Microbiology Society}},
  series       = {{Microbial Genomics}},
  title        = {{Comparative analysis of mitochondrion-related organelles in anaerobic amoebozoans}},
  url          = {{http://dx.doi.org/10.1099/mgen.0.001143}},
  doi          = {{10.1099/mgen.0.001143}},
  volume       = {{9}},
  year         = {{2023}},
}