Soluble M1 protein of Streptococcus pyogenes triggers potent T cell activation.
(2008) In Cellular Microbiology 10(2). p.404-414- Abstract
- Streptococcus pyogenes of the M1 serotype is commonly associated with large outbreaks of invasive streptococcal infections and development of streptococcal toxic shock syndrome (STSS). The pathogenesis behind these infections is believed to involve bacterial superantigens that induce potent inflammatory responses, but the reason why strains of the M1 serotype are over-represented in STSS is still not understood. In the present investigation, we show that a highly purified soluble form of the M1 protein from S. pyogenes, which lacks the membrane-spanning region, is a potent inducer of T cell proliferation and release of Th1 type cytokines. M1 protein-evoked T cell proliferation was HLA class II-dependent but not MHC-restricted, did not... (More)
- Streptococcus pyogenes of the M1 serotype is commonly associated with large outbreaks of invasive streptococcal infections and development of streptococcal toxic shock syndrome (STSS). The pathogenesis behind these infections is believed to involve bacterial superantigens that induce potent inflammatory responses, but the reason why strains of the M1 serotype are over-represented in STSS is still not understood. In the present investigation, we show that a highly purified soluble form of the M1 protein from S. pyogenes, which lacks the membrane-spanning region, is a potent inducer of T cell proliferation and release of Th1 type cytokines. M1 protein-evoked T cell proliferation was HLA class II-dependent but not MHC-restricted, did not require intracellular processing and was Vβ-restricted. Extensive mass spectrometry studies indicated that there were no other detectable proteins in the preparation. Taken together, our data demonstrate that soluble M1 protein is a novel streptococcal superantigen, which likely contributes to the excessive T cell activation and hyperinflammatory response seen in severe invasive streptococcal infections. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/607482
- author
- Påhlman, Lisa
LU
; Olin, Anders
LU
; Darenberg, Jessica
; Mörgelin, Matthias
LU
; Kotb, Malak
; Herwald, Heiko
LU
and Norrby-Teglund, Anna
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Cellular Microbiology
- volume
- 10
- issue
- 2
- pages
- 404 - 414
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000252205900013
- scopus:38049100562
- ISSN
- 1462-5814
- DOI
- 10.1111/j.1462-5822.2007.01053.x
- language
- English
- LU publication?
- yes
- id
- ace3555e-065c-4699-a20c-dd42211b6f8c (old id 607482)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17900297&dopt=Abstract
- date added to LUP
- 2016-04-01 11:51:32
- date last changed
- 2022-03-13 01:44:52
@article{ace3555e-065c-4699-a20c-dd42211b6f8c, abstract = {{Streptococcus pyogenes of the M1 serotype is commonly associated with large outbreaks of invasive streptococcal infections and development of streptococcal toxic shock syndrome (STSS). The pathogenesis behind these infections is believed to involve bacterial superantigens that induce potent inflammatory responses, but the reason why strains of the M1 serotype are over-represented in STSS is still not understood. In the present investigation, we show that a highly purified soluble form of the M1 protein from S. pyogenes, which lacks the membrane-spanning region, is a potent inducer of T cell proliferation and release of Th1 type cytokines. M1 protein-evoked T cell proliferation was HLA class II-dependent but not MHC-restricted, did not require intracellular processing and was Vβ-restricted. Extensive mass spectrometry studies indicated that there were no other detectable proteins in the preparation. Taken together, our data demonstrate that soluble M1 protein is a novel streptococcal superantigen, which likely contributes to the excessive T cell activation and hyperinflammatory response seen in severe invasive streptococcal infections.}}, author = {{Påhlman, Lisa and Olin, Anders and Darenberg, Jessica and Mörgelin, Matthias and Kotb, Malak and Herwald, Heiko and Norrby-Teglund, Anna}}, issn = {{1462-5814}}, language = {{eng}}, number = {{2}}, pages = {{404--414}}, publisher = {{Wiley-Blackwell}}, series = {{Cellular Microbiology}}, title = {{Soluble M1 protein of Streptococcus pyogenes triggers potent T cell activation.}}, url = {{http://dx.doi.org/10.1111/j.1462-5822.2007.01053.x}}, doi = {{10.1111/j.1462-5822.2007.01053.x}}, volume = {{10}}, year = {{2008}}, }