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Soluble M1 protein of Streptococcus pyogenes triggers potent T cell activation.

Påhlman, Lisa LU ; Olin, Anders LU ; Darenberg, Jessica; Mörgelin, Matthias LU ; Kotb, Malak; Herwald, Heiko LU and Norrby-Teglund, Anna (2008) In Cellular Microbiology 10(2). p.404-414
Abstract
Streptococcus pyogenes of the M1 serotype is commonly associated with large outbreaks of invasive streptococcal infections and development of streptococcal toxic shock syndrome (STSS). The pathogenesis behind these infections is believed to involve bacterial superantigens that induce potent inflammatory responses, but the reason why strains of the M1 serotype are over-represented in STSS is still not understood. In the present investigation, we show that a highly purified soluble form of the M1 protein from S. pyogenes, which lacks the membrane-spanning region, is a potent inducer of T cell proliferation and release of Th1 type cytokines. M1 protein-evoked T cell proliferation was HLA class II-dependent but not MHC-restricted, did not... (More)
Streptococcus pyogenes of the M1 serotype is commonly associated with large outbreaks of invasive streptococcal infections and development of streptococcal toxic shock syndrome (STSS). The pathogenesis behind these infections is believed to involve bacterial superantigens that induce potent inflammatory responses, but the reason why strains of the M1 serotype are over-represented in STSS is still not understood. In the present investigation, we show that a highly purified soluble form of the M1 protein from S. pyogenes, which lacks the membrane-spanning region, is a potent inducer of T cell proliferation and release of Th1 type cytokines. M1 protein-evoked T cell proliferation was HLA class II-dependent but not MHC-restricted, did not require intracellular processing and was Vβ-restricted. Extensive mass spectrometry studies indicated that there were no other detectable proteins in the preparation. Taken together, our data demonstrate that soluble M1 protein is a novel streptococcal superantigen, which likely contributes to the excessive T cell activation and hyperinflammatory response seen in severe invasive streptococcal infections. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Cellular Microbiology
volume
10
issue
2
pages
404 - 414
publisher
Wiley-Blackwell
external identifiers
  • wos:000252205900013
  • scopus:38049100562
ISSN
1462-5814
DOI
10.1111/j.1462-5822.2007.01053.x
language
English
LU publication?
yes
id
ace3555e-065c-4699-a20c-dd42211b6f8c (old id 607482)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17900297&dopt=Abstract
date added to LUP
2008-08-05 12:57:42
date last changed
2017-05-21 03:33:36
@article{ace3555e-065c-4699-a20c-dd42211b6f8c,
  abstract     = {Streptococcus pyogenes of the M1 serotype is commonly associated with large outbreaks of invasive streptococcal infections and development of streptococcal toxic shock syndrome (STSS). The pathogenesis behind these infections is believed to involve bacterial superantigens that induce potent inflammatory responses, but the reason why strains of the M1 serotype are over-represented in STSS is still not understood. In the present investigation, we show that a highly purified soluble form of the M1 protein from S. pyogenes, which lacks the membrane-spanning region, is a potent inducer of T cell proliferation and release of Th1 type cytokines. M1 protein-evoked T cell proliferation was HLA class II-dependent but not MHC-restricted, did not require intracellular processing and was Vβ-restricted. Extensive mass spectrometry studies indicated that there were no other detectable proteins in the preparation. Taken together, our data demonstrate that soluble M1 protein is a novel streptococcal superantigen, which likely contributes to the excessive T cell activation and hyperinflammatory response seen in severe invasive streptococcal infections.},
  author       = {Påhlman, Lisa and Olin, Anders and Darenberg, Jessica and Mörgelin, Matthias and Kotb, Malak and Herwald, Heiko and Norrby-Teglund, Anna},
  issn         = {1462-5814},
  language     = {eng},
  number       = {2},
  pages        = {404--414},
  publisher    = {Wiley-Blackwell},
  series       = {Cellular Microbiology},
  title        = {Soluble M1 protein of Streptococcus pyogenes triggers potent T cell activation.},
  url          = {http://dx.doi.org/10.1111/j.1462-5822.2007.01053.x},
  volume       = {10},
  year         = {2008},
}