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Human model neurons in studies of brain cell damage and neural repair.

Paquet-Durand, Francois LU and Bicker, Gerd (2007) In Current Molecular Medicine 7(6). p.541-554
Abstract
Disorders of the central nervous system are a major concern in modern human societies. Studies of these disorders require the use of suitable model systems that accurately reproduce the human situation. In this article we focus on the possibilities of using the human NT-2 teratocarcinoma cell line for studies on neuronal differentiation, cellular function and neurodegeneration. Neurons generated from undifferentiated NT-2 precursor cells show neuronal morphology, express neuronal markers, exhibit action potentials and have the advantage of homogeneous cellular composition of clonally derived cells. They release a number of different neurotransmitters, respond to stimulation with glutamate, gamma-amino-butyric acid, and nitric oxide, and... (More)
Disorders of the central nervous system are a major concern in modern human societies. Studies of these disorders require the use of suitable model systems that accurately reproduce the human situation. In this article we focus on the possibilities of using the human NT-2 teratocarcinoma cell line for studies on neuronal differentiation, cellular function and neurodegeneration. Neurons generated from undifferentiated NT-2 precursor cells show neuronal morphology, express neuronal markers, exhibit action potentials and have the advantage of homogeneous cellular composition of clonally derived cells. They release a number of different neurotransmitters, respond to stimulation with glutamate, gamma-amino-butyric acid, and nitric oxide, and form functional synapses in culture. Depending on the differentiation protocol, NT-2 cells also have the capacity to develop into glial cells. Different neuronal differentiation procedures and biological properties of NT-2 neurons are described in the text. In transplantation experiments, differentiated NT-2 neurons integrated successfully into the nervous systems of both experimental animals and human patients without evidence for tumor formation, underlining their value for both basic research and clinical applications. We discuss some potential applications in the fields of basic research, drug discovery, and therapy of CNS damage with particular emphasis on neuronal transplantation and different cell death mechanisms in neuronal degeneration. Grafting of NT-2 neurons has been shown to effectively reverse functional defects in animal disease models. Moreover, an ongoing phase 2 randomized clinical trial indicates the safety and feasibility of NT2 neuron transplantation for the treatment of human patients with cerebral stroke. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
neuronal replacement, excitotoxicity, neurodegeneration, calcium, NT2, Ntera, stroke
in
Current Molecular Medicine
volume
7
issue
6
pages
541 - 554
publisher
Bentham Science Publishers
external identifiers
  • wos:000250493700002
  • scopus:35448951796
ISSN
1566-5240
language
English
LU publication?
yes
id
3a8161ba-f512-465f-945f-002bc8bea4ba (old id 607508)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17896991&dopt=Abstract
http://www.ingentaconnect.com/content/ben/cmm/2007/00000007/00000006/art00002
date added to LUP
2007-12-19 12:20:15
date last changed
2017-03-12 04:08:23
@article{3a8161ba-f512-465f-945f-002bc8bea4ba,
  abstract     = {Disorders of the central nervous system are a major concern in modern human societies. Studies of these disorders require the use of suitable model systems that accurately reproduce the human situation. In this article we focus on the possibilities of using the human NT-2 teratocarcinoma cell line for studies on neuronal differentiation, cellular function and neurodegeneration. Neurons generated from undifferentiated NT-2 precursor cells show neuronal morphology, express neuronal markers, exhibit action potentials and have the advantage of homogeneous cellular composition of clonally derived cells. They release a number of different neurotransmitters, respond to stimulation with glutamate, gamma-amino-butyric acid, and nitric oxide, and form functional synapses in culture. Depending on the differentiation protocol, NT-2 cells also have the capacity to develop into glial cells. Different neuronal differentiation procedures and biological properties of NT-2 neurons are described in the text. In transplantation experiments, differentiated NT-2 neurons integrated successfully into the nervous systems of both experimental animals and human patients without evidence for tumor formation, underlining their value for both basic research and clinical applications. We discuss some potential applications in the fields of basic research, drug discovery, and therapy of CNS damage with particular emphasis on neuronal transplantation and different cell death mechanisms in neuronal degeneration. Grafting of NT-2 neurons has been shown to effectively reverse functional defects in animal disease models. Moreover, an ongoing phase 2 randomized clinical trial indicates the safety and feasibility of NT2 neuron transplantation for the treatment of human patients with cerebral stroke.},
  author       = {Paquet-Durand, Francois and Bicker, Gerd},
  issn         = {1566-5240},
  keyword      = {neuronal replacement,excitotoxicity,neurodegeneration,calcium,NT2,Ntera,stroke},
  language     = {eng},
  number       = {6},
  pages        = {541--554},
  publisher    = {Bentham Science Publishers},
  series       = {Current Molecular Medicine},
  title        = {Human model neurons in studies of brain cell damage and neural repair.},
  volume       = {7},
  year         = {2007},
}