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Lower proliferation rate in metastatic effusion mesothelial cells than in benign effusions.

Rehnberg, Johanna; Zendehrokh, Nooreldin LU and Dejmek, Annika LU (2007) In Analytical and Quantitative Cytology and Histology 29(4). p.217-220
Abstract
OBJECTIVE: To determine the proliferation rates of mesothelial cells in metastatic and benign effusions. STUDY DESIGN: Immunohistochemistry was performed on formalin-fixed pellets from 16 malignant and 9 benign clinical effusions. Dual staining with antibodies against Ki-67 (MIB-1) and desmin was applied to all effusions to differentiate between benign mesothelial cells and malignant cells, and the proportions of desmin+/Ki-67+ and desmin+/Ki-67- cells were calculated. RESULTS: In 7 malignant effusions no proliferating mesothelial cells were found, whereas some rate of proliferation could always be demonstrated in mesothelial cells in the benign effusions. Further, the median proportions of proliferating cells, malignant 2% vs. benign 11%,... (More)
OBJECTIVE: To determine the proliferation rates of mesothelial cells in metastatic and benign effusions. STUDY DESIGN: Immunohistochemistry was performed on formalin-fixed pellets from 16 malignant and 9 benign clinical effusions. Dual staining with antibodies against Ki-67 (MIB-1) and desmin was applied to all effusions to differentiate between benign mesothelial cells and malignant cells, and the proportions of desmin+/Ki-67+ and desmin+/Ki-67- cells were calculated. RESULTS: In 7 malignant effusions no proliferating mesothelial cells were found, whereas some rate of proliferation could always be demonstrated in mesothelial cells in the benign effusions. Further, the median proportions of proliferating cells, malignant 2% vs. benign 11%, differed significantly. CONCLUSIONS: To our knowledge this finding has not been previously described, and it may have implications for both cytologic diagnosis and the understanding of tumor biology and the interaction between tumor cells and mesothelial cells. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
proliferation, mesothelial cells, malignancy, effusion, Ki-67
in
Analytical and Quantitative Cytology and Histology
volume
29
issue
4
pages
217 - 220
publisher
Science Printers
external identifiers
  • wos:000248821800004
  • scopus:34548159946
ISSN
0884-6812
language
English
LU publication?
yes
id
27c68fbe-b462-43dd-846b-89785aa53c53 (old id 607683)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17879629&dopt=Abstract
date added to LUP
2007-12-14 10:49:20
date last changed
2017-01-01 07:17:27
@article{27c68fbe-b462-43dd-846b-89785aa53c53,
  abstract     = {OBJECTIVE: To determine the proliferation rates of mesothelial cells in metastatic and benign effusions. STUDY DESIGN: Immunohistochemistry was performed on formalin-fixed pellets from 16 malignant and 9 benign clinical effusions. Dual staining with antibodies against Ki-67 (MIB-1) and desmin was applied to all effusions to differentiate between benign mesothelial cells and malignant cells, and the proportions of desmin+/Ki-67+ and desmin+/Ki-67- cells were calculated. RESULTS: In 7 malignant effusions no proliferating mesothelial cells were found, whereas some rate of proliferation could always be demonstrated in mesothelial cells in the benign effusions. Further, the median proportions of proliferating cells, malignant 2% vs. benign 11%, differed significantly. CONCLUSIONS: To our knowledge this finding has not been previously described, and it may have implications for both cytologic diagnosis and the understanding of tumor biology and the interaction between tumor cells and mesothelial cells.},
  author       = {Rehnberg, Johanna and Zendehrokh, Nooreldin and Dejmek, Annika},
  issn         = {0884-6812},
  keyword      = {proliferation,mesothelial cells,malignancy,effusion,Ki-67},
  language     = {eng},
  number       = {4},
  pages        = {217--220},
  publisher    = {Science Printers},
  series       = {Analytical and Quantitative Cytology and Histology},
  title        = {Lower proliferation rate in metastatic effusion mesothelial cells than in benign effusions.},
  volume       = {29},
  year         = {2007},
}