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Delineating the specificity of an IgE-encoding transcriptome.

Persson, Helena LU ; Sadegh, Mardjaneh Karbalaei; Greiff, Lennart LU and Ohlin, Mats LU (2007) In Journal of Allergy and Clinical Immunology 120(5). p.1186-1192
Abstract
Background



Although much is known about the reactivity of polyclonal populations of antibodies targeting the wide array of allergens produced by timothy (Phleum pratense) and other grass species, little is known about the finer details at the level of individual antibody specificities.

Objective



We sought to investigate the IgE repertoire as it occurs in a patient with grass pollen allergy.

Methods



For this purpose, a human IgE library was used, constructed from peripheral blood B cells of an individual with timothy allergy. The library was screened by using phage display against a panel of 6 timothy allergens (Phl p 1, Phl p 2, Phl p 4, Phl p 5, Phl p 6, and Phl p... (More)
Background



Although much is known about the reactivity of polyclonal populations of antibodies targeting the wide array of allergens produced by timothy (Phleum pratense) and other grass species, little is known about the finer details at the level of individual antibody specificities.

Objective



We sought to investigate the IgE repertoire as it occurs in a patient with grass pollen allergy.

Methods



For this purpose, a human IgE library was used, constructed from peripheral blood B cells of an individual with timothy allergy. The library was screened by using phage display against a panel of 6 timothy allergens (Phl p 1, Phl p 2, Phl p 4, Phl p 5, Phl p 6, and Phl p 11).

Results



Highly diverse antibody fragments with respect to gene usage were identified. The binders were specific for their respective target antigen, except for clones selected on Phl p 6 that also recognized Phl p 5, most likely reflecting the high sequence homology between these allergens. Interestingly, by using this approach, we were able to determine the specificity of more than 25% of all IgE-producing transcripts in this individual with allergy.

Conclusion



The human IgE repertoire is produced by a limited number of highly related B-cell clones and as such is restricted in its recognition of a limited number of antigens.

Clinical implications



Human allergen-specific antibodies can, by defining the specificity of IgE responses, aid in the development of allergy vaccines or even by themselves be used in passive immunotherapy. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
antibody library, Allergy, antibody repertoire, diversity, grass pollen allergen, IgE, specificity, phage display
in
Journal of Allergy and Clinical Immunology
volume
120
issue
5
pages
1186 - 1192
publisher
Elsevier
external identifiers
  • wos:000250973400031
  • scopus:35649021829
ISSN
1097-6825
DOI
10.1016/j.jaci.2007.06.041
language
English
LU publication?
yes
id
1c6a53ea-9f9b-4e5f-9211-41657ce3b811 (old id 607912)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17825890&dopt=Abstract
date added to LUP
2007-11-26 09:52:29
date last changed
2017-04-23 04:16:06
@article{1c6a53ea-9f9b-4e5f-9211-41657ce3b811,
  abstract     = {Background<br/><br>
<br/><br>
Although much is known about the reactivity of polyclonal populations of antibodies targeting the wide array of allergens produced by timothy (Phleum pratense) and other grass species, little is known about the finer details at the level of individual antibody specificities.<br/><br>
Objective<br/><br>
<br/><br>
We sought to investigate the IgE repertoire as it occurs in a patient with grass pollen allergy.<br/><br>
Methods<br/><br>
<br/><br>
For this purpose, a human IgE library was used, constructed from peripheral blood B cells of an individual with timothy allergy. The library was screened by using phage display against a panel of 6 timothy allergens (Phl p 1, Phl p 2, Phl p 4, Phl p 5, Phl p 6, and Phl p 11).<br/><br>
Results<br/><br>
<br/><br>
Highly diverse antibody fragments with respect to gene usage were identified. The binders were specific for their respective target antigen, except for clones selected on Phl p 6 that also recognized Phl p 5, most likely reflecting the high sequence homology between these allergens. Interestingly, by using this approach, we were able to determine the specificity of more than 25% of all IgE-producing transcripts in this individual with allergy.<br/><br>
Conclusion<br/><br>
<br/><br>
The human IgE repertoire is produced by a limited number of highly related B-cell clones and as such is restricted in its recognition of a limited number of antigens.<br/><br>
Clinical implications<br/><br>
<br/><br>
Human allergen-specific antibodies can, by defining the specificity of IgE responses, aid in the development of allergy vaccines or even by themselves be used in passive immunotherapy.},
  author       = {Persson, Helena and Sadegh, Mardjaneh Karbalaei and Greiff, Lennart and Ohlin, Mats},
  issn         = {1097-6825},
  keyword      = {antibody library,Allergy,antibody repertoire,diversity,grass pollen allergen,IgE,specificity,phage display},
  language     = {eng},
  number       = {5},
  pages        = {1186--1192},
  publisher    = {Elsevier},
  series       = {Journal of Allergy and Clinical Immunology},
  title        = {Delineating the specificity of an IgE-encoding transcriptome.},
  url          = {http://dx.doi.org/10.1016/j.jaci.2007.06.041},
  volume       = {120},
  year         = {2007},
}