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The major basement membrane components localize to the chondrocyte pericellular matrix - A cartilage basement membrane equivalent?

Kvist, Alexander LU ; Nyström, Alexander LU ; Hultenby, Kjell; Sasaki, Takako; Talts, Jan F and Aspberg, Anders LU (2008) In Matrix Biology 27(1). p.22-33
Abstract
In this study, we demonstrate that articular cartilage chondrocytes are surrounded by the defining basement membrane proteins laminin, collagen type IV, nidogen and perlecan, and suggest that these form the functional equivalent of a basement membrane. We found by real-time PCR that mouse chondrocytes express these four cardinal components of basement membranes and demonstrated by immunohistochemistry that the proteins are present in bovine and mouse cartilage tissues and are deposited in a thin pericellular structure. Immunoelectron microscopy confirmed high laminin concentration in the pericellular matrix. In cartilage from newborn mice, basement membrane components are widespread in the territorial and interterritorial matrix, while in... (More)
In this study, we demonstrate that articular cartilage chondrocytes are surrounded by the defining basement membrane proteins laminin, collagen type IV, nidogen and perlecan, and suggest that these form the functional equivalent of a basement membrane. We found by real-time PCR that mouse chondrocytes express these four cardinal components of basement membranes and demonstrated by immunohistochemistry that the proteins are present in bovine and mouse cartilage tissues and are deposited in a thin pericellular structure. Immunoelectron microscopy confirmed high laminin concentration in the pericellular matrix. In cartilage from newborn mice, basement membrane components are widespread in the territorial and interterritorial matrix, while in mature cartilage of adult mice the basement membrane components are localized mainly to a narrow pericellular zone. With progression into old age, this layer becomes less distinct, especially in areas of obvious mechanical attrition. Interestingly, individual laminin subunits were located in different zones of the cartilage, with laminin α1 showing preferential localization around a select population of superficial layer chondrocytes. We propose that the chondrocyte, like several other cell types of mesenchymal origin, is surrounded by the functional equivalent of a basement membrane. This structure is presumably involved in maintaining chondrocyte phenotype and viability and may well allow a new understanding of cartilage development and provide clues to the progression of degenerative joint disorders. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Matrix Biology
volume
27
issue
1
pages
22 - 33
publisher
Elsevier
external identifiers
  • wos:000253404200004
  • scopus:38549149791
ISSN
1569-1802
DOI
10.1016/j.matbio.2007.07.007
language
English
LU publication?
yes
id
7c08a1a0-5fdd-42cf-b8e9-81fde307576b (old id 607918)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17825545&dopt=Abstract
date added to LUP
2008-08-06 13:45:28
date last changed
2017-09-24 04:09:42
@article{7c08a1a0-5fdd-42cf-b8e9-81fde307576b,
  abstract     = {In this study, we demonstrate that articular cartilage chondrocytes are surrounded by the defining basement membrane proteins laminin, collagen type IV, nidogen and perlecan, and suggest that these form the functional equivalent of a basement membrane. We found by real-time PCR that mouse chondrocytes express these four cardinal components of basement membranes and demonstrated by immunohistochemistry that the proteins are present in bovine and mouse cartilage tissues and are deposited in a thin pericellular structure. Immunoelectron microscopy confirmed high laminin concentration in the pericellular matrix. In cartilage from newborn mice, basement membrane components are widespread in the territorial and interterritorial matrix, while in mature cartilage of adult mice the basement membrane components are localized mainly to a narrow pericellular zone. With progression into old age, this layer becomes less distinct, especially in areas of obvious mechanical attrition. Interestingly, individual laminin subunits were located in different zones of the cartilage, with laminin α1 showing preferential localization around a select population of superficial layer chondrocytes. We propose that the chondrocyte, like several other cell types of mesenchymal origin, is surrounded by the functional equivalent of a basement membrane. This structure is presumably involved in maintaining chondrocyte phenotype and viability and may well allow a new understanding of cartilage development and provide clues to the progression of degenerative joint disorders.},
  author       = {Kvist, Alexander and Nyström, Alexander and Hultenby, Kjell and Sasaki, Takako and Talts, Jan F and Aspberg, Anders},
  issn         = {1569-1802},
  language     = {eng},
  number       = {1},
  pages        = {22--33},
  publisher    = {Elsevier},
  series       = {Matrix Biology},
  title        = {The major basement membrane components localize to the chondrocyte pericellular matrix - A cartilage basement membrane equivalent?},
  url          = {http://dx.doi.org/10.1016/j.matbio.2007.07.007},
  volume       = {27},
  year         = {2008},
}