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Solubilization of sphingomyelin vesicles by addition of a bile salt.

Cárdenas, Marité ; Schillén, Karin LU orcid ; Alfredsson, Viveka LU ; Duan, Rui-Dong ; Nyberg, Lena and Arnebrant, Thomas (2008) In Chemistry and Physics of Lipids 151(Sep 25). p.10-17
Abstract
The interactions of the bile salt sodium taurocholate (TC) in 50mM Trizma-HCl buffer and 150mM NaCl (pH 9) at 37 degrees C with membranes composed of sphingomyelin (SM) were studied by dynamic light scattering, cryogenic transmission electron microscopy (cryo-TEM) and turbidity measurements. Small unilamellar SM vesicles were prepared by extrusion. Below the CMC of TC, taurocholate addition leads to vesicle growth due to incorporation of the taurocholate molecules into the vesicle bilayer. At around half the CMC of the bile salt, the SM vesicles are transformed into SM/TC mixed worm-like micelles, which are visualized by cryo-TEM for the first time. Further increase in the taurocholate concentration leads to the rupture of these structures... (More)
The interactions of the bile salt sodium taurocholate (TC) in 50mM Trizma-HCl buffer and 150mM NaCl (pH 9) at 37 degrees C with membranes composed of sphingomyelin (SM) were studied by dynamic light scattering, cryogenic transmission electron microscopy (cryo-TEM) and turbidity measurements. Small unilamellar SM vesicles were prepared by extrusion. Below the CMC of TC, taurocholate addition leads to vesicle growth due to incorporation of the taurocholate molecules into the vesicle bilayer. At around half the CMC of the bile salt, the SM vesicles are transformed into SM/TC mixed worm-like micelles, which are visualized by cryo-TEM for the first time. Further increase in the taurocholate concentration leads to the rupture of these structures into small spherical micelles. Interestingly, large non-spherical micelles were also identified for pure taurocholate solutions. Similar threadlike structures have been reported earlier for the bile salt sodium taurodeoxycholate [Rich, A., Blow, D., 1958. Nature 182, 1777; Blow, D.M., Rich, A., 1960. J. Am. Chem. Soc. 82, 3566-3571; Galantini, L., Giglio, E., La Mesa, C., Viorel-Pavel, N., Punzo, F., 2002. Langmuir 18, 2812] and for mixtures of taurocholate and phosphatidylcholate [Ulmius, J., Lindblom, G., Wennerström, H., Johansson, L.B.-A., Fontel, K., Söderman, O., Ardvisson, G., 1982. Biochemistry 21, 1553; Hjelm, R.P., Thiyagarajan, P., Alkan-Onyuksel, H., 1992. J. Phys. Chem. 96, 8653] as determined by various scattering methods. (Less)
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Chemistry and Physics of Lipids
volume
151
issue
Sep 25
pages
10 - 17
publisher
Elsevier
external identifiers
  • wos:000253023500002
  • scopus:36448949859
  • pmid:17963701
ISSN
0009-3084
DOI
10.1016/j.chemphyslip.2007.09.002
language
English
LU publication?
yes
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Article in Press, Corrected Proof - Note to users
id
23ab266f-a32a-4867-bd70-994720e8506a (old id 608287)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17963701&dopt=Abstract
date added to LUP
2016-04-01 13:18:45
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2022-03-13 23:18:49
@article{23ab266f-a32a-4867-bd70-994720e8506a,
  abstract     = {{The interactions of the bile salt sodium taurocholate (TC) in 50mM Trizma-HCl buffer and 150mM NaCl (pH 9) at 37 degrees C with membranes composed of sphingomyelin (SM) were studied by dynamic light scattering, cryogenic transmission electron microscopy (cryo-TEM) and turbidity measurements. Small unilamellar SM vesicles were prepared by extrusion. Below the CMC of TC, taurocholate addition leads to vesicle growth due to incorporation of the taurocholate molecules into the vesicle bilayer. At around half the CMC of the bile salt, the SM vesicles are transformed into SM/TC mixed worm-like micelles, which are visualized by cryo-TEM for the first time. Further increase in the taurocholate concentration leads to the rupture of these structures into small spherical micelles. Interestingly, large non-spherical micelles were also identified for pure taurocholate solutions. Similar threadlike structures have been reported earlier for the bile salt sodium taurodeoxycholate [Rich, A., Blow, D., 1958. Nature 182, 1777; Blow, D.M., Rich, A., 1960. J. Am. Chem. Soc. 82, 3566-3571; Galantini, L., Giglio, E., La Mesa, C., Viorel-Pavel, N., Punzo, F., 2002. Langmuir 18, 2812] and for mixtures of taurocholate and phosphatidylcholate [Ulmius, J., Lindblom, G., Wennerström, H., Johansson, L.B.-A., Fontel, K., Söderman, O., Ardvisson, G., 1982. Biochemistry 21, 1553; Hjelm, R.P., Thiyagarajan, P., Alkan-Onyuksel, H., 1992. J. Phys. Chem. 96, 8653] as determined by various scattering methods.}},
  author       = {{Cárdenas, Marité and Schillén, Karin and Alfredsson, Viveka and Duan, Rui-Dong and Nyberg, Lena and Arnebrant, Thomas}},
  issn         = {{0009-3084}},
  language     = {{eng}},
  number       = {{Sep 25}},
  pages        = {{10--17}},
  publisher    = {{Elsevier}},
  series       = {{Chemistry and Physics of Lipids}},
  title        = {{Solubilization of sphingomyelin vesicles by addition of a bile salt.}},
  url          = {{http://dx.doi.org/10.1016/j.chemphyslip.2007.09.002}},
  doi          = {{10.1016/j.chemphyslip.2007.09.002}},
  volume       = {{151}},
  year         = {{2008}},
}