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Preparation and application of polymer-entrapped enzymes and microorganisms in microbial transformation processes with special reference to steroid 11-β-hydroxylation and Δ1-dehydrogenation

Mosbach, Klaus LU and Larsson, Per-Olof LU (1970) In Biotechnology and Bioengineering 12(1). p.19-27
Abstract



Fungal cells from Curvularia lunata were entrapped in
a crosslinked polyacrylamide gel. The gel-cells obtained as granules
were applied in the microbial transformation of Reichstein compound S
leading to cortisol through an 11-β-hydroxylation step. Some kinetic
studies of this conversion using gel-cells were carried out. In
addition, it was shown that gel-cell granules which had lost part of
their 11-β-hydroxylase activity on storage could be reactivated yielding
preparations with increased activity.


From Corynebacterium simplex a steroid dehydrogenase catalyzing the... (More)




Fungal cells from Curvularia lunata were entrapped in
a crosslinked polyacrylamide gel. The gel-cells obtained as granules
were applied in the microbial transformation of Reichstein compound S
leading to cortisol through an 11-β-hydroxylation step. Some kinetic
studies of this conversion using gel-cells were carried out. In
addition, it was shown that gel-cell granules which had lost part of
their 11-β-hydroxylase activity on storage could be reactivated yielding
preparations with increased activity.


From Corynebacterium simplex a steroid dehydrogenase catalyzing the Δ1-
dehydrogenation of cortisol leading to prednisolone was isolated and
partially purified. The preparation was entrapped in a crosslinked
polyacrylamide gel and the gel-enzyme granules obtained used in steroid
dehydrogenation processes.


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author
and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biotechnology and Bioengineering
volume
12
issue
1
pages
9 pages
publisher
John Wiley & Sons Inc.
external identifiers
  • scopus:0014714669
ISSN
1097-0290
DOI
10.1002/bit.260120103
language
English
LU publication?
yes
id
609b5a04-bd3d-4b2a-b916-d74351a6e1e1
date added to LUP
2024-06-23 22:41:38
date last changed
2024-09-16 14:19:12
@article{609b5a04-bd3d-4b2a-b916-d74351a6e1e1,
  abstract     = {{<div class="article-section__content en main"><br>
         <br>
         <br>
         <p>Fungal cells from <i>Curvularia lunata</i> were entrapped in<br>
 a crosslinked polyacrylamide gel. The gel-cells obtained as granules <br>
were applied in the microbial transformation of Reichstein compound S <br>
leading to cortisol through an 11-β-hydroxylation step. Some kinetic <br>
studies of this conversion using gel-cells were carried out. In <br>
addition, it was shown that gel-cell granules which had lost part of <br>
their 11-β-hydroxylase activity on storage could be reactivated yielding<br>
 preparations with increased activity.</p><br>
         <p>From <i>Corynebacterium simplex</i> a steroid dehydrogenase catalyzing the Δ<sup>1</sup>-<br>
 dehydrogenation of cortisol leading to prednisolone was isolated and <br>
partially purified. The preparation was entrapped in a crosslinked <br>
polyacrylamide gel and the gel-enzyme granules obtained used in steroid <br>
dehydrogenation processes.</p><br>
         </div>}},
  author       = {{Mosbach, Klaus and Larsson, Per-Olof}},
  issn         = {{1097-0290}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{19--27}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{Biotechnology and Bioengineering}},
  title        = {{Preparation and application of polymer-entrapped enzymes and microorganisms in microbial transformation processes with special reference to steroid 11-β-hydroxylation and Δ<sup>1</sup>-dehydrogenation}},
  url          = {{http://dx.doi.org/10.1002/bit.260120103}},
  doi          = {{10.1002/bit.260120103}},
  volume       = {{12}},
  year         = {{1970}},
}