Preparation and application of polymer-entrapped enzymes and microorganisms in microbial transformation processes with special reference to steroid 11-β-hydroxylation and Δ1-dehydrogenation
(1970) In Biotechnology and Bioengineering 12(1). p.19-27- Abstract
Fungal cells from Curvularia lunata were entrapped in
a crosslinked polyacrylamide gel. The gel-cells obtained as granules
were applied in the microbial transformation of Reichstein compound S
leading to cortisol through an 11-β-hydroxylation step. Some kinetic
studies of this conversion using gel-cells were carried out. In
addition, it was shown that gel-cell granules which had lost part of
their 11-β-hydroxylase activity on storage could be reactivated yielding
preparations with increased activity.
From Corynebacterium simplex a steroid dehydrogenase catalyzing the... (More)
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Fungal cells from Curvularia lunata were entrapped in
a crosslinked polyacrylamide gel. The gel-cells obtained as granules
were applied in the microbial transformation of Reichstein compound S
leading to cortisol through an 11-β-hydroxylation step. Some kinetic
studies of this conversion using gel-cells were carried out. In
addition, it was shown that gel-cell granules which had lost part of
their 11-β-hydroxylase activity on storage could be reactivated yielding
preparations with increased activity.
From Corynebacterium simplex a steroid dehydrogenase catalyzing the Δ1-
dehydrogenation of cortisol leading to prednisolone was isolated and
partially purified. The preparation was entrapped in a crosslinked
polyacrylamide gel and the gel-enzyme granules obtained used in steroid
dehydrogenation processes.
Please use this url to cite or link to this publication: https://lup.lub.lu.se/record/609b5a04-bd3d-4b2a-b916-d74351a6e1e1- author
- Mosbach, Klaus LU and Larsson, Per-Olof LU
- organization
- publishing date
- 1970
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Biotechnology and Bioengineering
- volume
- 12
- issue
- 1
- pages
- 9 pages
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- scopus:0014714669
- ISSN
- 1097-0290
- DOI
- 10.1002/bit.260120103
- language
- English
- LU publication?
- yes
- id
- 609b5a04-bd3d-4b2a-b916-d74351a6e1e1
- date added to LUP
- 2024-06-23 22:41:38
- date last changed
- 2024-09-16 14:19:12
@article{609b5a04-bd3d-4b2a-b916-d74351a6e1e1, abstract = {{<div class="article-section__content en main"><br> <br> <br> <p>Fungal cells from <i>Curvularia lunata</i> were entrapped in<br> a crosslinked polyacrylamide gel. The gel-cells obtained as granules <br> were applied in the microbial transformation of Reichstein compound S <br> leading to cortisol through an 11-β-hydroxylation step. Some kinetic <br> studies of this conversion using gel-cells were carried out. In <br> addition, it was shown that gel-cell granules which had lost part of <br> their 11-β-hydroxylase activity on storage could be reactivated yielding<br> preparations with increased activity.</p><br> <p>From <i>Corynebacterium simplex</i> a steroid dehydrogenase catalyzing the Δ<sup>1</sup>-<br> dehydrogenation of cortisol leading to prednisolone was isolated and <br> partially purified. The preparation was entrapped in a crosslinked <br> polyacrylamide gel and the gel-enzyme granules obtained used in steroid <br> dehydrogenation processes.</p><br> </div>}}, author = {{Mosbach, Klaus and Larsson, Per-Olof}}, issn = {{1097-0290}}, language = {{eng}}, number = {{1}}, pages = {{19--27}}, publisher = {{John Wiley & Sons Inc.}}, series = {{Biotechnology and Bioengineering}}, title = {{Preparation and application of polymer-entrapped enzymes and microorganisms in microbial transformation processes with special reference to steroid 11-β-hydroxylation and Δ<sup>1</sup>-dehydrogenation}}, url = {{http://dx.doi.org/10.1002/bit.260120103}}, doi = {{10.1002/bit.260120103}}, volume = {{12}}, year = {{1970}}, }