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Advancing time-resolved structural biology: latest strategies in cryo-EM and X-ray crystallography

Banari, Amir ; Samanta, Amit K. ; Munke, Anna LU orcid ; Laugks, Tim ; Bajt, Saša ; Grünewald, Kay ; Marlovits, Thomas C. ; Küpper, Jochen ; Maia, Filipe R.N.C. and Chapman, Henry N. , et al. (2025) In Nature Methods
Abstract
Structural biology offers a window into the functionality of molecular machines in health and disease. A fundamental challenge lies in capturing both the high-resolution structural details and dynamic changes that are essential for function. The high-resolution methods of X-ray crystallography and electron cryo-microscopy (cryo-EM) are mainly used to study ensembles of static conformations but can also capture crucial dynamic transition states. Here, we review the latest strategies and advancements in time-resolved structural biology with a focus on capturing dynamic changes. We describe recent technology developments for time-resolved sample preparation and delivery in the cryo-EM and X-ray fields and explore how these technologies could... (More)
Structural biology offers a window into the functionality of molecular machines in health and disease. A fundamental challenge lies in capturing both the high-resolution structural details and dynamic changes that are essential for function. The high-resolution methods of X-ray crystallography and electron cryo-microscopy (cryo-EM) are mainly used to study ensembles of static conformations but can also capture crucial dynamic transition states. Here, we review the latest strategies and advancements in time-resolved structural biology with a focus on capturing dynamic changes. We describe recent technology developments for time-resolved sample preparation and delivery in the cryo-EM and X-ray fields and explore how these technologies could mutually benefit each other to advance our understanding of biology at the molecular and atomic scales. (Less)
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publishing date
type
Contribution to journal
publication status
epub
subject
keywords
time-resolved, dynamics, cryo-EM, crystallography, X-ray difffraction
in
Nature Methods
publisher
Nature Publishing Group
external identifiers
  • pmid:40312512
ISSN
1548-7105
DOI
10.1038/s41592-025-02659-6
language
English
LU publication?
no
id
60a83dab-c0b8-4bbb-ad59-68426547b5c5
date added to LUP
2025-05-04 16:42:03
date last changed
2025-05-08 13:40:05
@article{60a83dab-c0b8-4bbb-ad59-68426547b5c5,
  abstract     = {{Structural biology offers a window into the functionality of molecular machines in health and disease. A fundamental challenge lies in capturing both the high-resolution structural details and dynamic changes that are essential for function. The high-resolution methods of X-ray crystallography and electron cryo-microscopy (cryo-EM) are mainly used to study ensembles of static conformations but can also capture crucial dynamic transition states. Here, we review the latest strategies and advancements in time-resolved structural biology with a focus on capturing dynamic changes. We describe recent technology developments for time-resolved sample preparation and delivery in the cryo-EM and X-ray fields and explore how these technologies could mutually benefit each other to advance our understanding of biology at the molecular and atomic scales.}},
  author       = {{Banari, Amir and Samanta, Amit K. and Munke, Anna and Laugks, Tim and Bajt, Saša and Grünewald, Kay and Marlovits, Thomas C. and Küpper, Jochen and Maia, Filipe R.N.C. and Chapman, Henry N. and Oberthür, Dominik and Seuring, Carolin}},
  issn         = {{1548-7105}},
  keywords     = {{time-resolved; dynamics; cryo-EM; crystallography; X-ray difffraction}},
  language     = {{eng}},
  month        = {{05}},
  publisher    = {{Nature Publishing Group}},
  series       = {{Nature Methods}},
  title        = {{Advancing time-resolved structural biology: latest strategies in cryo-EM and X-ray crystallography}},
  url          = {{http://dx.doi.org/10.1038/s41592-025-02659-6}},
  doi          = {{10.1038/s41592-025-02659-6}},
  year         = {{2025}},
}