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Rrp6 is recruited to transcribed genes and accompanies the spliced mRNA to the nuclear pore

Hessle, Viktoria ; von Euler, Anne ; González de Valdivia, Ernesto LU orcid and Visa, Neus (2012) In RNA 18(8). p.74-1466
Abstract

Rrp6 is an exoribonuclease involved in the quality control of mRNA biogenesis. We have analyzed the association of Rrp6 with the Balbiani ring pre-mRNPs of Chironomus tentans to obtain insight into the role of Rrp6 in splicing surveillance. Rrp6 is recruited to transcribed genes and its distribution along the genes does not correlate with the positions of exons and introns. In the nucleoplasm, Rrp6 is bound to both unspliced and spliced transcripts. Rrp6 is released from the mRNPs in the vicinity of the nuclear pore before nucleo-cytoplasmic translocation. We show that Rrp6 is associated with newly synthesized transcripts during all the nuclear steps of gene expression and is associated with the transcripts independently of their... (More)

Rrp6 is an exoribonuclease involved in the quality control of mRNA biogenesis. We have analyzed the association of Rrp6 with the Balbiani ring pre-mRNPs of Chironomus tentans to obtain insight into the role of Rrp6 in splicing surveillance. Rrp6 is recruited to transcribed genes and its distribution along the genes does not correlate with the positions of exons and introns. In the nucleoplasm, Rrp6 is bound to both unspliced and spliced transcripts. Rrp6 is released from the mRNPs in the vicinity of the nuclear pore before nucleo-cytoplasmic translocation. We show that Rrp6 is associated with newly synthesized transcripts during all the nuclear steps of gene expression and is associated with the transcripts independently of their splicing status. These observations suggest that the quality control of pre-mRNA splicing is not based on the selective recruitment of the exoribonuclease Rrp6 to unprocessed mRNAs.

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author
; ; and
publishing date
type
Contribution to journal
publication status
published
keywords
Animals, Blotting, Western, Cell Nucleus, Cells, Cultured, Chironomidae, Chromatin Immunoprecipitation, Cytoplasm, Drosophila Proteins, Drosophila melanogaster, Exons, Exosome Multienzyme Ribonuclease Complex, Fluorescent Antibody Technique, Introns, Nuclear Pore, Nuclear Proteins, RNA Precursors, RNA Splicing, RNA, Messenger, Rabbits, Ribonucleoproteins, Salivary Glands, Transcription, Genetic, Journal Article, Research Support, Non-U.S. Gov't
in
RNA
volume
18
issue
8
pages
9 pages
publisher
Cold Spring Harbor Laboratory Press (CSHL)
external identifiers
  • scopus:84864072645
  • pmid:22745224
ISSN
1355-8382
DOI
10.1261/rna.032045.111
language
English
LU publication?
no
id
60b89f21-9b6f-4deb-9d12-c79d9bf1cc4d
date added to LUP
2018-01-12 17:02:26
date last changed
2024-01-29 09:29:44
@article{60b89f21-9b6f-4deb-9d12-c79d9bf1cc4d,
  abstract     = {{<p>Rrp6 is an exoribonuclease involved in the quality control of mRNA biogenesis. We have analyzed the association of Rrp6 with the Balbiani ring pre-mRNPs of Chironomus tentans to obtain insight into the role of Rrp6 in splicing surveillance. Rrp6 is recruited to transcribed genes and its distribution along the genes does not correlate with the positions of exons and introns. In the nucleoplasm, Rrp6 is bound to both unspliced and spliced transcripts. Rrp6 is released from the mRNPs in the vicinity of the nuclear pore before nucleo-cytoplasmic translocation. We show that Rrp6 is associated with newly synthesized transcripts during all the nuclear steps of gene expression and is associated with the transcripts independently of their splicing status. These observations suggest that the quality control of pre-mRNA splicing is not based on the selective recruitment of the exoribonuclease Rrp6 to unprocessed mRNAs.</p>}},
  author       = {{Hessle, Viktoria and von Euler, Anne and González de Valdivia, Ernesto and Visa, Neus}},
  issn         = {{1355-8382}},
  keywords     = {{Animals; Blotting, Western; Cell Nucleus; Cells, Cultured; Chironomidae; Chromatin Immunoprecipitation; Cytoplasm; Drosophila Proteins; Drosophila melanogaster; Exons; Exosome Multienzyme Ribonuclease Complex; Fluorescent Antibody Technique; Introns; Nuclear Pore; Nuclear Proteins; RNA Precursors; RNA Splicing; RNA, Messenger; Rabbits; Ribonucleoproteins; Salivary Glands; Transcription, Genetic; Journal Article; Research Support, Non-U.S. Gov't}},
  language     = {{eng}},
  number       = {{8}},
  pages        = {{74--1466}},
  publisher    = {{Cold Spring Harbor Laboratory Press (CSHL)}},
  series       = {{RNA}},
  title        = {{Rrp6 is recruited to transcribed genes and accompanies the spliced mRNA to the nuclear pore}},
  url          = {{http://dx.doi.org/10.1261/rna.032045.111}},
  doi          = {{10.1261/rna.032045.111}},
  volume       = {{18}},
  year         = {{2012}},
}