The alveolar surface is lined by a coherent liquid-crystalline phase
(2002) Conference on Lipid and Polymer-Lipid Systems, 2002 120. p.28-34- Abstract
- The existence of a uniform phase at the alveolar surface is demonstrated by polarizing microscopy and cryogenic transmission electron microscopy (cryo-TEM). Rabbit lungs were opened and the alveolar surface film was directly deposited on optical microscope coverslips and on electron microscope grids. In the polarizing microscope, the alveolar surface material deposited exhibits a uniform birefringence, which shows that the alveolar surface is lined by a coherent liquid-crystalline phase; a surface phase. Cryo-TEM, with reduced risks of artifacts compared to conventional electron microscopy, shows the existence of a mechanically stable and continuous surface structure of regularly spaced bilayers oriented along the grid surface over large... (More)
- The existence of a uniform phase at the alveolar surface is demonstrated by polarizing microscopy and cryogenic transmission electron microscopy (cryo-TEM). Rabbit lungs were opened and the alveolar surface film was directly deposited on optical microscope coverslips and on electron microscope grids. In the polarizing microscope, the alveolar surface material deposited exhibits a uniform birefringence, which shows that the alveolar surface is lined by a coherent liquid-crystalline phase; a surface phase. Cryo-TEM, with reduced risks of artifacts compared to conventional electron microscopy, shows the existence of a mechanically stable and continuous surface structure of regularly spaced bilayers oriented along the grid surface over large distances (microns). The periodicity observed by cryo-TEM indicates that the surface phase has the same inner organization as the ultrastructure known as tubular myelin (TM). Two alternative structures of TM are considered: the traditional crossed bilayer structure and a bilayer structure free from intersections according to the so-called crossed layers of parallels (CLP) minimal surface. Evidence presently available favors the CLP minimal surface structure alternative. The implication of the existence of a surface phase on the barrier function of the alveolar surface zone is finally considered (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/611242
- author
- Larsson, M. ; Larsson, K. and Wollmer, Per LU
- organization
- publishing date
- 2002
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- crossed layers, bilayer structure, crossed bilayer structure, tubular myelin, ultrastructure, grid surface, regularly spaced bilayers, continuous surface structure, birefringence, alveolar surface material, polarizing microscope, electron microscope grids, coherent liquid-crystalline phase, optical microscope coverslips, polarizing microscopy, cryogenic transmission electron microscopy, rabbit lungs, alveolar surface film, barrier function
- host publication
- Progress in Colloid & Polymer Science
- volume
- 120
- pages
- 28 - 34
- conference name
- Conference on Lipid and Polymer-Lipid Systems, 2002
- conference location
- Chia Laguna, Italy
- conference dates
- 2002-10-01 - 2002-10-02
- external identifiers
-
- wos:000177564900004
- other:CODEN: PCPSD7
- scopus:0036955296
- ISSN
- 0340-255X
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Clinical Physiology (013242300), Clinical Physiology and Nuclear Medicine Unit (013242320)
- id
- 0bc58347-3e40-4821-a200-d85c49a6179b (old id 611242)
- date added to LUP
- 2016-04-04 09:20:58
- date last changed
- 2023-09-05 22:25:10
@inproceedings{0bc58347-3e40-4821-a200-d85c49a6179b,
abstract = {{The existence of a uniform phase at the alveolar surface is demonstrated by polarizing microscopy and cryogenic transmission electron microscopy (cryo-TEM). Rabbit lungs were opened and the alveolar surface film was directly deposited on optical microscope coverslips and on electron microscope grids. In the polarizing microscope, the alveolar surface material deposited exhibits a uniform birefringence, which shows that the alveolar surface is lined by a coherent liquid-crystalline phase; a surface phase. Cryo-TEM, with reduced risks of artifacts compared to conventional electron microscopy, shows the existence of a mechanically stable and continuous surface structure of regularly spaced bilayers oriented along the grid surface over large distances (microns). The periodicity observed by cryo-TEM indicates that the surface phase has the same inner organization as the ultrastructure known as tubular myelin (TM). Two alternative structures of TM are considered: the traditional crossed bilayer structure and a bilayer structure free from intersections according to the so-called crossed layers of parallels (CLP) minimal surface. Evidence presently available favors the CLP minimal surface structure alternative. The implication of the existence of a surface phase on the barrier function of the alveolar surface zone is finally considered}},
author = {{Larsson, M. and Larsson, K. and Wollmer, Per}},
booktitle = {{Progress in Colloid & Polymer Science}},
issn = {{0340-255X}},
keywords = {{crossed layers; bilayer structure; crossed bilayer structure; tubular myelin; ultrastructure; grid surface; regularly spaced bilayers; continuous surface structure; birefringence; alveolar surface material; polarizing microscope; electron microscope grids; coherent liquid-crystalline phase; optical microscope coverslips; polarizing microscopy; cryogenic transmission electron microscopy; rabbit lungs; alveolar surface film; barrier function}},
language = {{eng}},
pages = {{28--34}},
title = {{The alveolar surface is lined by a coherent liquid-crystalline phase}},
volume = {{120}},
year = {{2002}},
}