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The alveolar surface is lined by a coherent liquid-crystalline phase

Larsson, M. ; Larsson, K. and Wollmer, Per LU (2002) Conference on Lipid and Polymer-Lipid Systems, 2002 120. p.28-34
Abstract
The existence of a uniform phase at the alveolar surface is demonstrated by polarizing microscopy and cryogenic transmission electron microscopy (cryo-TEM). Rabbit lungs were opened and the alveolar surface film was directly deposited on optical microscope coverslips and on electron microscope grids. In the polarizing microscope, the alveolar surface material deposited exhibits a uniform birefringence, which shows that the alveolar surface is lined by a coherent liquid-crystalline phase; a surface phase. Cryo-TEM, with reduced risks of artifacts compared to conventional electron microscopy, shows the existence of a mechanically stable and continuous surface structure of regularly spaced bilayers oriented along the grid surface over large... (More)
The existence of a uniform phase at the alveolar surface is demonstrated by polarizing microscopy and cryogenic transmission electron microscopy (cryo-TEM). Rabbit lungs were opened and the alveolar surface film was directly deposited on optical microscope coverslips and on electron microscope grids. In the polarizing microscope, the alveolar surface material deposited exhibits a uniform birefringence, which shows that the alveolar surface is lined by a coherent liquid-crystalline phase; a surface phase. Cryo-TEM, with reduced risks of artifacts compared to conventional electron microscopy, shows the existence of a mechanically stable and continuous surface structure of regularly spaced bilayers oriented along the grid surface over large distances (microns). The periodicity observed by cryo-TEM indicates that the surface phase has the same inner organization as the ultrastructure known as tubular myelin (TM). Two alternative structures of TM are considered: the traditional crossed bilayer structure and a bilayer structure free from intersections according to the so-called crossed layers of parallels (CLP) minimal surface. Evidence presently available favors the CLP minimal surface structure alternative. The implication of the existence of a surface phase on the barrier function of the alveolar surface zone is finally considered (Less)
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author
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organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
crossed layers, bilayer structure, crossed bilayer structure, tubular myelin, ultrastructure, grid surface, regularly spaced bilayers, continuous surface structure, birefringence, alveolar surface material, polarizing microscope, electron microscope grids, coherent liquid-crystalline phase, optical microscope coverslips, polarizing microscopy, cryogenic transmission electron microscopy, rabbit lungs, alveolar surface film, barrier function
host publication
Progress in Colloid & Polymer Science
volume
120
pages
28 - 34
conference name
Conference on Lipid and Polymer-Lipid Systems, 2002
conference location
Chia Laguna, Italy
conference dates
2002-10-01 - 2002-10-02
external identifiers
  • wos:000177564900004
  • other:CODEN: PCPSD7
  • scopus:0036955296
ISSN
0340-255X
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Clinical Physiology (013242300), Clinical Physiology and Nuclear Medicine Unit (013242320)
id
0bc58347-3e40-4821-a200-d85c49a6179b (old id 611242)
date added to LUP
2016-04-04 09:20:58
date last changed
2020-01-12 20:50:21
@inproceedings{0bc58347-3e40-4821-a200-d85c49a6179b,
  abstract     = {The existence of a uniform phase at the alveolar surface is demonstrated by polarizing microscopy and cryogenic transmission electron microscopy (cryo-TEM). Rabbit lungs were opened and the alveolar surface film was directly deposited on optical microscope coverslips and on electron microscope grids. In the polarizing microscope, the alveolar surface material deposited exhibits a uniform birefringence, which shows that the alveolar surface is lined by a coherent liquid-crystalline phase; a surface phase. Cryo-TEM, with reduced risks of artifacts compared to conventional electron microscopy, shows the existence of a mechanically stable and continuous surface structure of regularly spaced bilayers oriented along the grid surface over large distances (microns). The periodicity observed by cryo-TEM indicates that the surface phase has the same inner organization as the ultrastructure known as tubular myelin (TM). Two alternative structures of TM are considered: the traditional crossed bilayer structure and a bilayer structure free from intersections according to the so-called crossed layers of parallels (CLP) minimal surface. Evidence presently available favors the CLP minimal surface structure alternative. The implication of the existence of a surface phase on the barrier function of the alveolar surface zone is finally considered},
  author       = {Larsson, M. and Larsson, K. and Wollmer, Per},
  booktitle    = {Progress in Colloid & Polymer Science},
  issn         = {0340-255X},
  language     = {eng},
  pages        = {28--34},
  title        = {The alveolar surface is lined by a coherent liquid-crystalline phase},
  volume       = {120},
  year         = {2002},
}