Locus-specific detection of HLA-DQ and -DR antigens by antibodies against synthetic N-terminal octapeptides of the β chain
Deufel, Thomas ; Grove, Anne ; Kofod, Hans and Lernmark, Åke LU
(1985)
In FEBS Letters
189(2).
p.329-337
- Abstract
Antibodies against synthetic peptides representing the class-II antigen HLA-DR and -DQ β chain N-terminal sequences were prepared in rabbits. The two octapeptides only share two amino acids and enzyme-linked immuno-assays showed the antisera only to bind to its own antigen. Both peptide antisera detected a 29 kDa component in immunoblots of Raji and AL-34 cell plasma membrane proteins separated by SDS gel electrophoresis. The binding of either N-terminal peptide antiserum was selectively inhibited only by the peptide used as antigen. Indirect immunofluorescence.analysis by flow cytofluorometry showed specific surface immunofluorescence in 1:100-1:1000 dilutions in lymphoblastoid and blood mononucleated cells. In the latter the binding... (More)
Antibodies against synthetic peptides representing the class-II antigen HLA-DR and -DQ β chain N-terminal sequences were prepared in rabbits. The two octapeptides only share two amino acids and enzyme-linked immuno-assays showed the antisera only to bind to its own antigen. Both peptide antisera detected a 29 kDa component in immunoblots of Raji and AL-34 cell plasma membrane proteins separated by SDS gel electrophoresis. The binding of either N-terminal peptide antiserum was selectively inhibited only by the peptide used as antigen. Indirect immunofluorescence.analysis by flow cytofluorometry showed specific surface immunofluorescence in 1:100-1:1000 dilutions in lymphoblastoid and blood mononucleated cells. In the latter the binding was primarily confined to monocytes and a subpopulation of lymphocytes. It is concluded that locus-specific immunological reagents to distinguish between β chains of HLA-DR and -DQ have been prepared by the preparation by the production of antibodies against the N-terminal sequences of each polypeptide.
(Less)
- author
- Deufel, Thomas
; Grove, Anne
; Kofod, Hans
and Lernmark, Åke
LU
- publishing date
- 1985-09-23
- type
- Contribution to journal
- publication status
- published
- keywords
- ELISA, Flow cytofluorometry, HLA class II antigen, Immunoblotting bl]
- in
- FEBS Letters
- volume
- 189
- issue
- 2
- pages
- 9 pages
- publisher
- Wiley-Blackwell
- external identifiers
-
- scopus:0021926776
- pmid:2995123
- ISSN
- 0014-5793
- DOI
- 10.1016/0014-5793(85)81050-4
- language
- English
- LU publication?
- no
- id
- 613a4ea7-36ea-4a06-b674-f30eb6be5577
- date added to LUP
- 2019-09-16 12:38:59
- date last changed
- 2024-03-13 08:06:46
@article{613a4ea7-36ea-4a06-b674-f30eb6be5577,
abstract = {{<p>Antibodies against synthetic peptides representing the class-II antigen HLA-DR and -DQ β chain N-terminal sequences were prepared in rabbits. The two octapeptides only share two amino acids and enzyme-linked immuno-assays showed the antisera only to bind to its own antigen. Both peptide antisera detected a 29 kDa component in immunoblots of Raji and AL-34 cell plasma membrane proteins separated by SDS gel electrophoresis. The binding of either N-terminal peptide antiserum was selectively inhibited only by the peptide used as antigen. Indirect immunofluorescence.analysis by flow cytofluorometry showed specific surface immunofluorescence in 1:100-1:1000 dilutions in lymphoblastoid and blood mononucleated cells. In the latter the binding was primarily confined to monocytes and a subpopulation of lymphocytes. It is concluded that locus-specific immunological reagents to distinguish between β chains of HLA-DR and -DQ have been prepared by the preparation by the production of antibodies against the N-terminal sequences of each polypeptide.</p>}},
author = {{Deufel, Thomas and Grove, Anne and Kofod, Hans and Lernmark, Åke}},
issn = {{0014-5793}},
keywords = {{ELISA; Flow cytofluorometry; HLA class II antigen; Immunoblotting bl]}},
language = {{eng}},
month = {{09}},
number = {{2}},
pages = {{329--337}},
publisher = {{Wiley-Blackwell}},
series = {{FEBS Letters}},
title = {{Locus-specific detection of HLA-DQ and -DR antigens by antibodies against synthetic N-terminal octapeptides of the β chain}},
url = {{http://dx.doi.org/10.1016/0014-5793(85)81050-4}},
doi = {{10.1016/0014-5793(85)81050-4}},
volume = {{189}},
year = {{1985}},
}