Long Non-coding RNA T-UCstem1 Controls Progenitor Proliferation and Neurogenesis in the Postnatal Mouse Olfactory Bulb through Interaction with miR-9
(2020) In Stem Cell Reports 15(4). p.836-844- Abstract
Neural stem cell populations generate a wide spectrum of neuronal and glial cell types in a highly ordered fashion. MicroRNAs are essential regulators of this process. T-UCstem1 is a long non-coding RNA containing an ultraconserved element, and in vitro analyses in pluripotent stem cells provided evidence that it regulates the balance between proliferation and differentiation. Here we investigate the in vivo function of T-UCstem1. We show that T-UCstem1 is expressed in the forebrain neurogenic lineage that generates interneurons for the postnatal olfactory bulb. Gain of function in neural stem cells increased progenitor proliferation at the expense of neuron production, whereas knockdown had the opposite effect. This regulatory function... (More)
Neural stem cell populations generate a wide spectrum of neuronal and glial cell types in a highly ordered fashion. MicroRNAs are essential regulators of this process. T-UCstem1 is a long non-coding RNA containing an ultraconserved element, and in vitro analyses in pluripotent stem cells provided evidence that it regulates the balance between proliferation and differentiation. Here we investigate the in vivo function of T-UCstem1. We show that T-UCstem1 is expressed in the forebrain neurogenic lineage that generates interneurons for the postnatal olfactory bulb. Gain of function in neural stem cells increased progenitor proliferation at the expense of neuron production, whereas knockdown had the opposite effect. This regulatory function is mediated by its interaction with miR-9-3p and miR-9-5p. Based thereon, we propose a mechanistic model for the role of T-UCstem1 in the dynamic regulation of neural progenitor proliferation during neurogenesis.
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- author
- Pascale, Emilia ; Beclin, Christophe ; Fiorenzano, Alessandro LU ; Andolfi, Gennaro ; Erni, Andrea ; De Falco, Sandro ; Minchiotti, Gabriella ; Cremer, Harold and Fico, Annalisa
- organization
- publishing date
- 2020-10-13
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- adult neurogenesis, lncRNAs, miR-9, olfactory bulb, postnatal neurogenesis, subventricular zone, T-UCstem1, ultraconserved elements
- in
- Stem Cell Reports
- volume
- 15
- issue
- 4
- pages
- 9 pages
- publisher
- Cell Press
- external identifiers
-
- pmid:32976763
- scopus:85092325628
- ISSN
- 2213-6711
- DOI
- 10.1016/j.stemcr.2020.08.009
- language
- English
- LU publication?
- yes
- id
- 615aeccd-3a92-4b39-9d26-e6c788277547
- date added to LUP
- 2020-11-02 15:43:01
- date last changed
- 2024-08-22 06:00:25
@article{615aeccd-3a92-4b39-9d26-e6c788277547, abstract = {{<p>Neural stem cell populations generate a wide spectrum of neuronal and glial cell types in a highly ordered fashion. MicroRNAs are essential regulators of this process. T-UCstem1 is a long non-coding RNA containing an ultraconserved element, and in vitro analyses in pluripotent stem cells provided evidence that it regulates the balance between proliferation and differentiation. Here we investigate the in vivo function of T-UCstem1. We show that T-UCstem1 is expressed in the forebrain neurogenic lineage that generates interneurons for the postnatal olfactory bulb. Gain of function in neural stem cells increased progenitor proliferation at the expense of neuron production, whereas knockdown had the opposite effect. This regulatory function is mediated by its interaction with miR-9-3p and miR-9-5p. Based thereon, we propose a mechanistic model for the role of T-UCstem1 in the dynamic regulation of neural progenitor proliferation during neurogenesis.</p>}}, author = {{Pascale, Emilia and Beclin, Christophe and Fiorenzano, Alessandro and Andolfi, Gennaro and Erni, Andrea and De Falco, Sandro and Minchiotti, Gabriella and Cremer, Harold and Fico, Annalisa}}, issn = {{2213-6711}}, keywords = {{adult neurogenesis; lncRNAs; miR-9; olfactory bulb; postnatal neurogenesis; subventricular zone; T-UCstem1; ultraconserved elements}}, language = {{eng}}, month = {{10}}, number = {{4}}, pages = {{836--844}}, publisher = {{Cell Press}}, series = {{Stem Cell Reports}}, title = {{Long Non-coding RNA T-UCstem1 Controls Progenitor Proliferation and Neurogenesis in the Postnatal Mouse Olfactory Bulb through Interaction with miR-9}}, url = {{http://dx.doi.org/10.1016/j.stemcr.2020.08.009}}, doi = {{10.1016/j.stemcr.2020.08.009}}, volume = {{15}}, year = {{2020}}, }