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Long Non-coding RNA T-UCstem1 Controls Progenitor Proliferation and Neurogenesis in the Postnatal Mouse Olfactory Bulb through Interaction with miR-9

Pascale, Emilia ; Beclin, Christophe ; Fiorenzano, Alessandro LU ; Andolfi, Gennaro ; Erni, Andrea ; De Falco, Sandro ; Minchiotti, Gabriella ; Cremer, Harold and Fico, Annalisa (2020) In Stem Cell Reports 15(4). p.836-844
Abstract

Neural stem cell populations generate a wide spectrum of neuronal and glial cell types in a highly ordered fashion. MicroRNAs are essential regulators of this process. T-UCstem1 is a long non-coding RNA containing an ultraconserved element, and in vitro analyses in pluripotent stem cells provided evidence that it regulates the balance between proliferation and differentiation. Here we investigate the in vivo function of T-UCstem1. We show that T-UCstem1 is expressed in the forebrain neurogenic lineage that generates interneurons for the postnatal olfactory bulb. Gain of function in neural stem cells increased progenitor proliferation at the expense of neuron production, whereas knockdown had the opposite effect. This regulatory function... (More)

Neural stem cell populations generate a wide spectrum of neuronal and glial cell types in a highly ordered fashion. MicroRNAs are essential regulators of this process. T-UCstem1 is a long non-coding RNA containing an ultraconserved element, and in vitro analyses in pluripotent stem cells provided evidence that it regulates the balance between proliferation and differentiation. Here we investigate the in vivo function of T-UCstem1. We show that T-UCstem1 is expressed in the forebrain neurogenic lineage that generates interneurons for the postnatal olfactory bulb. Gain of function in neural stem cells increased progenitor proliferation at the expense of neuron production, whereas knockdown had the opposite effect. This regulatory function is mediated by its interaction with miR-9-3p and miR-9-5p. Based thereon, we propose a mechanistic model for the role of T-UCstem1 in the dynamic regulation of neural progenitor proliferation during neurogenesis.

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author
; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
adult neurogenesis, lncRNAs, miR-9, olfactory bulb, postnatal neurogenesis, subventricular zone, T-UCstem1, ultraconserved elements
in
Stem Cell Reports
volume
15
issue
4
pages
9 pages
publisher
Cell Press
external identifiers
  • scopus:85092325628
  • pmid:32976763
ISSN
2213-6711
DOI
10.1016/j.stemcr.2020.08.009
language
English
LU publication?
yes
id
615aeccd-3a92-4b39-9d26-e6c788277547
date added to LUP
2020-11-02 15:43:01
date last changed
2024-05-01 19:02:36
@article{615aeccd-3a92-4b39-9d26-e6c788277547,
  abstract     = {{<p>Neural stem cell populations generate a wide spectrum of neuronal and glial cell types in a highly ordered fashion. MicroRNAs are essential regulators of this process. T-UCstem1 is a long non-coding RNA containing an ultraconserved element, and in vitro analyses in pluripotent stem cells provided evidence that it regulates the balance between proliferation and differentiation. Here we investigate the in vivo function of T-UCstem1. We show that T-UCstem1 is expressed in the forebrain neurogenic lineage that generates interneurons for the postnatal olfactory bulb. Gain of function in neural stem cells increased progenitor proliferation at the expense of neuron production, whereas knockdown had the opposite effect. This regulatory function is mediated by its interaction with miR-9-3p and miR-9-5p. Based thereon, we propose a mechanistic model for the role of T-UCstem1 in the dynamic regulation of neural progenitor proliferation during neurogenesis.</p>}},
  author       = {{Pascale, Emilia and Beclin, Christophe and Fiorenzano, Alessandro and Andolfi, Gennaro and Erni, Andrea and De Falco, Sandro and Minchiotti, Gabriella and Cremer, Harold and Fico, Annalisa}},
  issn         = {{2213-6711}},
  keywords     = {{adult neurogenesis; lncRNAs; miR-9; olfactory bulb; postnatal neurogenesis; subventricular zone; T-UCstem1; ultraconserved elements}},
  language     = {{eng}},
  month        = {{10}},
  number       = {{4}},
  pages        = {{836--844}},
  publisher    = {{Cell Press}},
  series       = {{Stem Cell Reports}},
  title        = {{Long Non-coding RNA T-UCstem1 Controls Progenitor Proliferation and Neurogenesis in the Postnatal Mouse Olfactory Bulb through Interaction with miR-9}},
  url          = {{http://dx.doi.org/10.1016/j.stemcr.2020.08.009}},
  doi          = {{10.1016/j.stemcr.2020.08.009}},
  volume       = {{15}},
  year         = {{2020}},
}