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Epigenome- and Transcriptome-wide Changes in Muscle Stem Cells from Low Birth Weight Men

Broholm, Christa ; Ribel-Madsen, Rasmus ; Hjort, Line LU ; Olsson, Anders Henrik LU ; Ahlers, Juliane Maria Dorothee ; Hansen, Ninna Schiøler ; Schrölkamp, Maren ; Gillberg, Linn LU ; Perfilyev, Alexander LU and Volkov, Petr LU , et al. (2019) In Endocrine Research
Abstract

Background: Being born with low birth weight (LBW) is a risk factor for muscle insulin resistance and type 2 diabetes (T2D), which may be mediated by epigenetic mechanisms programmed by the intrauterine environment. Epigenetic mechanisms exert their prime effects in developing cells. We hypothesized that muscle insulin resistance in LBW subjects may be due to early differential epigenomic and transcriptomic alterations in their immature muscle progenitor cells. Results: Muscle progenitor cells were obtained from 23 healthy young adult men born at term with LBW, and 15 BMI-matched normal birth weight (NBW) controls. The cells were subsequently cultured and differentiated into myotubes. DNA and RNA were harvested before and after... (More)

Background: Being born with low birth weight (LBW) is a risk factor for muscle insulin resistance and type 2 diabetes (T2D), which may be mediated by epigenetic mechanisms programmed by the intrauterine environment. Epigenetic mechanisms exert their prime effects in developing cells. We hypothesized that muscle insulin resistance in LBW subjects may be due to early differential epigenomic and transcriptomic alterations in their immature muscle progenitor cells. Results: Muscle progenitor cells were obtained from 23 healthy young adult men born at term with LBW, and 15 BMI-matched normal birth weight (NBW) controls. The cells were subsequently cultured and differentiated into myotubes. DNA and RNA were harvested before and after differentiation for genome-wide DNA methylation and RNA expression measurements. After correcting for multiple comparisons (q ≤ 0.05), 56 CpG sites were found to be significantly, differentially methylated in myoblasts from LBW compared with NBW men, of which the top five gene-annotated CpG sites (SKI, ARMCX3, NR5A2, NEUROG, ESRRG) previously have been associated to regulation of cholesterol, fatty acid and glucose metabolism and muscle development or hypertrophy. LBW men displayed markedly decreased myotube gene expression levels of the AMPK-repressing tyrosine kinase gene FYN and the histone deacetylase gene HDAC7. Silencing of FYN and HDAC7 was associated with impaired myotube formation, which for HDAC7 reduced muscle glucose uptake. Conclusions: The data provides evidence of impaired muscle development predisposing LBW individuals to T2D is linked to and potentially caused by distinct DNA methylation and transcriptional changes including down regulation of HDAC7 and FYN in their immature myoblast stem cells.

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@article{61621856-6bb7-49ed-8457-f9f9a2913e20,
  abstract     = {<p>Background: Being born with low birth weight (LBW) is a risk factor for muscle insulin resistance and type 2 diabetes (T2D), which may be mediated by epigenetic mechanisms programmed by the intrauterine environment. Epigenetic mechanisms exert their prime effects in developing cells. We hypothesized that muscle insulin resistance in LBW subjects may be due to early differential epigenomic and transcriptomic alterations in their immature muscle progenitor cells. Results: Muscle progenitor cells were obtained from 23 healthy young adult men born at term with LBW, and 15 BMI-matched normal birth weight (NBW) controls. The cells were subsequently cultured and differentiated into myotubes. DNA and RNA were harvested before and after differentiation for genome-wide DNA methylation and RNA expression measurements. After correcting for multiple comparisons (q ≤ 0.05), 56 CpG sites were found to be significantly, differentially methylated in myoblasts from LBW compared with NBW men, of which the top five gene-annotated CpG sites (SKI, ARMCX3, NR5A2, NEUROG, ESRRG) previously have been associated to regulation of cholesterol, fatty acid and glucose metabolism and muscle development or hypertrophy. LBW men displayed markedly decreased myotube gene expression levels of the AMPK-repressing tyrosine kinase gene FYN and the histone deacetylase gene HDAC7. Silencing of FYN and HDAC7 was associated with impaired myotube formation, which for HDAC7 reduced muscle glucose uptake. Conclusions: The data provides evidence of impaired muscle development predisposing LBW individuals to T2D is linked to and potentially caused by distinct DNA methylation and transcriptional changes including down regulation of HDAC7 and FYN in their immature myoblast stem cells.</p>},
  author       = {Broholm, Christa and Ribel-Madsen, Rasmus and Hjort, Line and Olsson, Anders Henrik and Ahlers, Juliane Maria Dorothee and Hansen, Ninna Schiøler and Schrölkamp, Maren and Gillberg, Linn and Perfilyev, Alexander and Volkov, Petr and Ling, Charlotte and Jørgensen, Sine W. and Mortensen, Brynjulf and Hingst, Janne and Wojtaszewski, Jørgen and Scheele, Camilla and Brøns, Charlotte and Pedersen, Bente Klarlund and Vaag, Allan},
  issn         = {0743-5800},
  language     = {eng},
  month        = {09},
  publisher    = {Taylor & Francis},
  series       = {Endocrine Research},
  title        = {Epigenome- and Transcriptome-wide Changes in Muscle Stem Cells from Low Birth Weight Men},
  url          = {http://dx.doi.org/10.1080/07435800.2019.1669160},
  doi          = {10.1080/07435800.2019.1669160},
  year         = {2019},
}