Advanced

Hunting down hydrogen : Applying neutron macromolecular crystallography to galectins

Logan, Derek T. LU (2019) In Biochemist 41(2). p.20-23
Abstract

Galectins are a medically important family of small, soluble carbohydrate-binding proteins that have been implicated in a wide variety of diseases, and are therefore targets for the development of new drugs. Many X-ray crystal structures of small molecules in complex with galectins have been determined and have given invaluable information on how natural ligands and synthetic inhibitors are recognized. However, until now the hydrogen-bonding patterns involved in ligand recognition have been a matter of informed guesswork, as X-ray crystallography can't reliably tell us where hydrogen atoms are positioned. We describe how neutron macromolecular crystallography has been applied to galectins for the first time to shed light on this... (More)

Galectins are a medically important family of small, soluble carbohydrate-binding proteins that have been implicated in a wide variety of diseases, and are therefore targets for the development of new drugs. Many X-ray crystal structures of small molecules in complex with galectins have been determined and have given invaluable information on how natural ligands and synthetic inhibitors are recognized. However, until now the hydrogen-bonding patterns involved in ligand recognition have been a matter of informed guesswork, as X-ray crystallography can't reliably tell us where hydrogen atoms are positioned. We describe how neutron macromolecular crystallography has been applied to galectins for the first time to shed light on this question.

(Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biochemist
volume
41
issue
2
pages
4 pages
external identifiers
  • scopus:85075172934
ISSN
0954-982X
DOI
10.1042/bio04102020
language
English
LU publication?
yes
id
61625fea-1f6f-4ff1-ba87-15331b14138b
date added to LUP
2019-12-10 11:22:29
date last changed
2020-01-13 02:35:28
@article{61625fea-1f6f-4ff1-ba87-15331b14138b,
  abstract     = {<p>Galectins are a medically important family of small, soluble carbohydrate-binding proteins that have been implicated in a wide variety of diseases, and are therefore targets for the development of new drugs. Many X-ray crystal structures of small molecules in complex with galectins have been determined and have given invaluable information on how natural ligands and synthetic inhibitors are recognized. However, until now the hydrogen-bonding patterns involved in ligand recognition have been a matter of informed guesswork, as X-ray crystallography can't reliably tell us where hydrogen atoms are positioned. We describe how neutron macromolecular crystallography has been applied to galectins for the first time to shed light on this question.</p>},
  author       = {Logan, Derek T.},
  issn         = {0954-982X},
  language     = {eng},
  month        = {04},
  number       = {2},
  pages        = {20--23},
  series       = {Biochemist},
  title        = {Hunting down hydrogen : Applying neutron macromolecular crystallography to galectins},
  url          = {http://dx.doi.org/10.1042/bio04102020},
  doi          = {10.1042/bio04102020},
  volume       = {41},
  year         = {2019},
}