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Stability of serum prostate-specific antigen determination across laboratory, assay, and storage time

Jacobsen, Steven J. ; Klee, George G. ; Lilja, Hans LU orcid ; Wright, George L. and Oesterling, Joseph E. (1995) In Urology 45(3). p.447-453
Abstract

Objectives: To understand the comparability of serum prostate-specific antigen (PSA) determinations across assays and storage time. Methods: Serum PSA levels were determined for men aged 40 to 79 years from the clinical subset of the Olmsted County Study of Urinary Symptoms and Health Status Among Men on fresh samples and after a median of 32 months on banked samples, frozen at -70 °C. Baseline serum PSA levels were determined by Tandem-R PSA assay. Follow-up levels on the banked samples were determined by the IMx PSA assay and a repeat Tandem-R PSA assay in a different laboratory and by an immunofluorometric PSA assay at another site. Results: The median serum PSA level determined by Tandem-R assay at baseline was 1.0 ng/mL (25th... (More)

Objectives: To understand the comparability of serum prostate-specific antigen (PSA) determinations across assays and storage time. Methods: Serum PSA levels were determined for men aged 40 to 79 years from the clinical subset of the Olmsted County Study of Urinary Symptoms and Health Status Among Men on fresh samples and after a median of 32 months on banked samples, frozen at -70 °C. Baseline serum PSA levels were determined by Tandem-R PSA assay. Follow-up levels on the banked samples were determined by the IMx PSA assay and a repeat Tandem-R PSA assay in a different laboratory and by an immunofluorometric PSA assay at another site. Results: The median serum PSA level determined by Tandem-R assay at baseline was 1.0 ng/mL (25th percentile, 0.6; 75th percentile, 1.7). The distributions of determination made by follow-up Tandem-R, IMx, and immunofluorometric analyses were essentially identical. Overall, the assays were highly correlated. The correlations between the baseline serum PSA determination and repeated Tandem-R, IMx, and immunofluorometric determinations were 0.96, 0.96, and 0.97, respectively (all P < 0.001). The median duration of frozen storage was 32 months (range, 26 to 39 months), and the correlations between baseline and follow-up determinations did not change when stratified by duration of storage. Conclusions: These data provide important reassurance about the use of serum PSA determinations obtained by different assays, in different laboratories, and in properly tored samples across time.

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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Urology
volume
45
issue
3
pages
447 - 453
publisher
Elsevier
external identifiers
  • scopus:0028892407
  • pmid:7533456
ISSN
0090-4295
DOI
10.1016/S0090-4295(99)80014-4
language
English
LU publication?
yes
id
61b8664b-10e0-46e2-a5a8-bbe207980b56
date added to LUP
2022-12-06 17:08:45
date last changed
2025-04-04 15:07:25
@article{61b8664b-10e0-46e2-a5a8-bbe207980b56,
  abstract     = {{<p>Objectives: To understand the comparability of serum prostate-specific antigen (PSA) determinations across assays and storage time. Methods: Serum PSA levels were determined for men aged 40 to 79 years from the clinical subset of the Olmsted County Study of Urinary Symptoms and Health Status Among Men on fresh samples and after a median of 32 months on banked samples, frozen at -70 °C. Baseline serum PSA levels were determined by Tandem-R PSA assay. Follow-up levels on the banked samples were determined by the IMx PSA assay and a repeat Tandem-R PSA assay in a different laboratory and by an immunofluorometric PSA assay at another site. Results: The median serum PSA level determined by Tandem-R assay at baseline was 1.0 ng/mL (25th percentile, 0.6; 75th percentile, 1.7). The distributions of determination made by follow-up Tandem-R, IMx, and immunofluorometric analyses were essentially identical. Overall, the assays were highly correlated. The correlations between the baseline serum PSA determination and repeated Tandem-R, IMx, and immunofluorometric determinations were 0.96, 0.96, and 0.97, respectively (all P &lt; 0.001). The median duration of frozen storage was 32 months (range, 26 to 39 months), and the correlations between baseline and follow-up determinations did not change when stratified by duration of storage. Conclusions: These data provide important reassurance about the use of serum PSA determinations obtained by different assays, in different laboratories, and in properly tored samples across time.</p>}},
  author       = {{Jacobsen, Steven J. and Klee, George G. and Lilja, Hans and Wright, George L. and Oesterling, Joseph E.}},
  issn         = {{0090-4295}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{447--453}},
  publisher    = {{Elsevier}},
  series       = {{Urology}},
  title        = {{Stability of serum prostate-specific antigen determination across laboratory, assay, and storage time}},
  url          = {{http://dx.doi.org/10.1016/S0090-4295(99)80014-4}},
  doi          = {{10.1016/S0090-4295(99)80014-4}},
  volume       = {{45}},
  year         = {{1995}},
}