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Structural Effects in the Analysis of Supported Lipid Bilayers by Time-of-Flight Secondary Ion Mass Spectrometry.

Prinz, Christelle LU ; Höök, Fredrik LU ; Malm, Jakob LU and Sjövall, Peter LU (2007) In Langmuir 23(15). p.8035-8041
Abstract
We contribute to the rapidly emerging interest in the application of time-of-flight secondary ion mass spectrometry (TOF-SIMS) for chemical analysis of biological materials by presenting a careful TOF-SIMS investigation of structurally different SiO2-supported phospholipid assemblies. Freeze-dried supported 1-oleoyl-2-palmitoyl-sn-glycero-3-phosphocholine (POPC) bilayers, Langmuir-Blodgett POPC monolayers, and disordered thick POPC films were investigated. Compared with the two latter structures, the supported bilayer showed a strong (5-10 times) enhancement in the yield of both the molecular and the dimer ion peaks of POPC, suggesting that the molecular peak may be used as a sensitive indicator for changes in the membrane structure and,... (More)
We contribute to the rapidly emerging interest in the application of time-of-flight secondary ion mass spectrometry (TOF-SIMS) for chemical analysis of biological materials by presenting a careful TOF-SIMS investigation of structurally different SiO2-supported phospholipid assemblies. Freeze-dried supported 1-oleoyl-2-palmitoyl-sn-glycero-3-phosphocholine (POPC) bilayers, Langmuir-Blodgett POPC monolayers, and disordered thick POPC films were investigated. Compared with the two latter structures, the supported bilayer showed a strong (5-10 times) enhancement in the yield of both the molecular and the dimer ion peaks of POPC, suggesting that the molecular peak may be used as a sensitive indicator for changes in the membrane structure and, in particular, an indicator for the presence of bilayer structures in, e.g., cell and tissue samples. The detection efficiency and the useful lateral resolution indicate that a lateral resolution of around 100 nm can be obtained on all structures by imaging the phosphocholine ion at 184 u using Bi<sub>3</sub><sup>+</sup> primary ions. For the chemically specific molecular peak at 760 u, the measured detection efficiencies correspond to a useful lateral resolution of around 2 m for the bilayer structure. The results are discussed in relation to recent dynamic SIMS (nano-SIMS) analysis of freeze-dried supported lipid bilayers, displaying similar or higher lateral resolution, but which in contrast to TOF-SIMS requires isotopic labeling of the analyzed lipids. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Langmuir
volume
23
issue
15
pages
8035 - 8041
publisher
The American Chemical Society
external identifiers
  • wos:000247893600025
  • scopus:34547381070
ISSN
0743-7463
DOI
10.1021/la7004634
language
English
LU publication?
yes
id
c3cc9771-39b1-4083-b567-bd92b40c192b (old id 620778)
date added to LUP
2007-12-14 09:33:49
date last changed
2017-10-01 03:43:55
@article{c3cc9771-39b1-4083-b567-bd92b40c192b,
  abstract     = {We contribute to the rapidly emerging interest in the application of time-of-flight secondary ion mass spectrometry (TOF-SIMS) for chemical analysis of biological materials by presenting a careful TOF-SIMS investigation of structurally different SiO2-supported phospholipid assemblies. Freeze-dried supported 1-oleoyl-2-palmitoyl-sn-glycero-3-phosphocholine (POPC) bilayers, Langmuir-Blodgett POPC monolayers, and disordered thick POPC films were investigated. Compared with the two latter structures, the supported bilayer showed a strong (5-10 times) enhancement in the yield of both the molecular and the dimer ion peaks of POPC, suggesting that the molecular peak may be used as a sensitive indicator for changes in the membrane structure and, in particular, an indicator for the presence of bilayer structures in, e.g., cell and tissue samples. The detection efficiency and the useful lateral resolution indicate that a lateral resolution of around 100 nm can be obtained on all structures by imaging the phosphocholine ion at 184 u using Bi&lt;sub&gt;3&lt;/sub&gt;&lt;sup&gt;+&lt;/sup&gt; primary ions. For the chemically specific molecular peak at 760 u, the measured detection efficiencies correspond to a useful lateral resolution of around 2 m for the bilayer structure. The results are discussed in relation to recent dynamic SIMS (nano-SIMS) analysis of freeze-dried supported lipid bilayers, displaying similar or higher lateral resolution, but which in contrast to TOF-SIMS requires isotopic labeling of the analyzed lipids.},
  author       = {Prinz, Christelle and Höök, Fredrik and Malm, Jakob and Sjövall, Peter},
  issn         = {0743-7463},
  language     = {eng},
  number       = {15},
  pages        = {8035--8041},
  publisher    = {The American Chemical Society},
  series       = {Langmuir},
  title        = {Structural Effects in the Analysis of Supported Lipid Bilayers by Time-of-Flight Secondary Ion Mass Spectrometry.},
  url          = {http://dx.doi.org/10.1021/la7004634},
  volume       = {23},
  year         = {2007},
}