Frequency domain optical resolution photoacoustic and fluorescence microscopy using a modulated laser diode
(2017) Photons Plus Ultrasound: Imaging and Sensing 2017 In Progress in Biomedical Optics and Imaging - Proceedings of SPIE 10064.- Abstract
In this paper a multimodal optical-resolution photoacoustic and fluorescence microscope in frequency domain is presented. Photoacoustic waves and modulated fluorescence are generated in chromophores by using a modulated diode laser. The photoacoustic waves, recorded with a hydrophone, and the fluorescence signals, acquired with an avalanche photodiode, are simultaneously measured using a lock-in technique. Two possibilities to optimize the signal-to-noise ratio are discussed. The first method is based on the optimization of the excitation waveform and it is argued why square-wave excitation is best. The second way to enhance the SNR is to optimize the modulation frequency. For modulation periods that are much shorter than the relaxation... (More)
In this paper a multimodal optical-resolution photoacoustic and fluorescence microscope in frequency domain is presented. Photoacoustic waves and modulated fluorescence are generated in chromophores by using a modulated diode laser. The photoacoustic waves, recorded with a hydrophone, and the fluorescence signals, acquired with an avalanche photodiode, are simultaneously measured using a lock-in technique. Two possibilities to optimize the signal-to-noise ratio are discussed. The first method is based on the optimization of the excitation waveform and it is argued why square-wave excitation is best. The second way to enhance the SNR is to optimize the modulation frequency. For modulation periods that are much shorter than the relaxation times of the excited chromophores, the photoacoustic signal scales linearly with the modulation frequency. We come to the conclusion that frequency-domain photoacoustic microscopy performed with modulation frequencies in the range of 100 MHz can compete with time-domain photoacoustic microscopy regarding the signal-to-noise ratio. The theoretical predictions are confirmed by experimental results. Additionally, images of stained and unstained biological samples are presented in order to demonstrate the capabilities of the multimodal imaging system.
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- author
- Langer, Gregor ; Langer, Andreas LU ; Buchegger, Bianca ; Jacak, Jaroslaw ; Klar, Thomas A. and Berer, Thomas
- publishing date
- 2017
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- Diode laser, Fluorescence microscopy, Frequency domain, Lock-in technique, Multimodal imaging, Optical resolution photoacoustic microscopy, Photoacoustic microscopy, Square wave excitation
- host publication
- Photons Plus Ultrasound : Imaging and Sensing 2017 - Imaging and Sensing 2017
- series title
- Progress in Biomedical Optics and Imaging - Proceedings of SPIE
- editor
- Oraevsky, Alexander A. and Wang, Lihong V.
- volume
- 10064
- article number
- 1006426
- publisher
- SPIE
- conference name
- Photons Plus Ultrasound: Imaging and Sensing 2017
- conference location
- San Francisco, United States
- conference dates
- 2017-01-29 - 2017-02-01
- external identifiers
-
- scopus:85018926035
- ISSN
- 1605-7422
- ISBN
- 9781510605695
- DOI
- 10.1117/12.2250861
- language
- English
- LU publication?
- no
- additional info
- Publisher Copyright: © 2017 SPIE. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.
- id
- 62c3d921-f8f9-4a8a-94e8-ce2833d2db9e
- date added to LUP
- 2021-03-15 22:33:43
- date last changed
- 2024-07-13 11:09:06
@inproceedings{62c3d921-f8f9-4a8a-94e8-ce2833d2db9e, abstract = {{<p>In this paper a multimodal optical-resolution photoacoustic and fluorescence microscope in frequency domain is presented. Photoacoustic waves and modulated fluorescence are generated in chromophores by using a modulated diode laser. The photoacoustic waves, recorded with a hydrophone, and the fluorescence signals, acquired with an avalanche photodiode, are simultaneously measured using a lock-in technique. Two possibilities to optimize the signal-to-noise ratio are discussed. The first method is based on the optimization of the excitation waveform and it is argued why square-wave excitation is best. The second way to enhance the SNR is to optimize the modulation frequency. For modulation periods that are much shorter than the relaxation times of the excited chromophores, the photoacoustic signal scales linearly with the modulation frequency. We come to the conclusion that frequency-domain photoacoustic microscopy performed with modulation frequencies in the range of 100 MHz can compete with time-domain photoacoustic microscopy regarding the signal-to-noise ratio. The theoretical predictions are confirmed by experimental results. Additionally, images of stained and unstained biological samples are presented in order to demonstrate the capabilities of the multimodal imaging system.</p>}}, author = {{Langer, Gregor and Langer, Andreas and Buchegger, Bianca and Jacak, Jaroslaw and Klar, Thomas A. and Berer, Thomas}}, booktitle = {{Photons Plus Ultrasound : Imaging and Sensing 2017}}, editor = {{Oraevsky, Alexander A. and Wang, Lihong V.}}, isbn = {{9781510605695}}, issn = {{1605-7422}}, keywords = {{Diode laser; Fluorescence microscopy; Frequency domain; Lock-in technique; Multimodal imaging; Optical resolution photoacoustic microscopy; Photoacoustic microscopy; Square wave excitation}}, language = {{eng}}, publisher = {{SPIE}}, series = {{Progress in Biomedical Optics and Imaging - Proceedings of SPIE}}, title = {{Frequency domain optical resolution photoacoustic and fluorescence microscopy using a modulated laser diode}}, url = {{http://dx.doi.org/10.1117/12.2250861}}, doi = {{10.1117/12.2250861}}, volume = {{10064}}, year = {{2017}}, }