DNA fragmentation and ultrastructural changes of degenerating cells in atherosclerotic lesions and smooth muscle cells exposed to oxidized LDL in vitro
(1997) In Arteriosclerosis, Thrombosis and Vascular Biology 17(10). p.2225-2231- Abstract
- Degeneration of smooth muscle cells in the fibrous cap of atherosclerotic lesions is an important factor in plaque rupture. Recent studies have suggested that many plaque cells are in a process of apoptosis as determined by positive deoxyribonucleotide-transferase-mediated dUTP end labeling. In this study, we demonstrate the existence of a colocalization between deoxyribonucleotide-transferase-mediated dUTP end labeling-positive smooth muscle cells and oxidized LDL immunoreactivity in human carotid plaques. Oxidized LDL was found to induce deoxyribonucleotide-transferase-mediated dUTP end labeling positivity in cultured human smooth muscle cells, but only in the presence of tumor necrosis factor-alpha and interferon-gamma. Electron... (More)
- Degeneration of smooth muscle cells in the fibrous cap of atherosclerotic lesions is an important factor in plaque rupture. Recent studies have suggested that many plaque cells are in a process of apoptosis as determined by positive deoxyribonucleotide-transferase-mediated dUTP end labeling. In this study, we demonstrate the existence of a colocalization between deoxyribonucleotide-transferase-mediated dUTP end labeling-positive smooth muscle cells and oxidized LDL immunoreactivity in human carotid plaques. Oxidized LDL was found to induce deoxyribonucleotide-transferase-mediated dUTP end labeling positivity in cultured human smooth muscle cells, but only in the presence of tumor necrosis factor-alpha and interferon-gamma. Electron microscopic analysis of cultured smooth muscle cells exposed to oxidized LDL in the absence of cytokines demonstrated cytoplasmic swelling and disruption of the plasma membrane, suggesting cell death by oncosis. Cells exposed to both oxidized LDL and cytokines were characterized by chromatin and cytoplasmic condensation compatible with cell death by apoptosis. These findings further support the notion that oxidized lipids play a role in plaque cell death. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1112575
- author
- Jovinge, Stefan LU ; Crisby, M ; Thyberg, J and Nilsson, J
- publishing date
- 1997
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- apoptosis, cell death, oxidized LDL, TNF{alpha}, {gamma}IFN
- in
- Arteriosclerosis, Thrombosis and Vascular Biology
- volume
- 17
- issue
- 10
- pages
- 2225 - 2231
- publisher
- Lippincott Williams & Wilkins
- external identifiers
-
- pmid:9351393
- scopus:0031456718
- ISSN
- 1524-4636
- language
- English
- LU publication?
- no
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Hematopoietic Stem Cell Laboratory (013022012)
- id
- 62d51113-cd03-44c8-b6be-9eec30bc8094 (old id 1112575)
- alternative location
- http://atvb.ahajournals.org/cgi/content/full/17/10/2225
- date added to LUP
- 2016-04-01 12:29:04
- date last changed
- 2022-04-05 22:54:57
@article{62d51113-cd03-44c8-b6be-9eec30bc8094, abstract = {{Degeneration of smooth muscle cells in the fibrous cap of atherosclerotic lesions is an important factor in plaque rupture. Recent studies have suggested that many plaque cells are in a process of apoptosis as determined by positive deoxyribonucleotide-transferase-mediated dUTP end labeling. In this study, we demonstrate the existence of a colocalization between deoxyribonucleotide-transferase-mediated dUTP end labeling-positive smooth muscle cells and oxidized LDL immunoreactivity in human carotid plaques. Oxidized LDL was found to induce deoxyribonucleotide-transferase-mediated dUTP end labeling positivity in cultured human smooth muscle cells, but only in the presence of tumor necrosis factor-alpha and interferon-gamma. Electron microscopic analysis of cultured smooth muscle cells exposed to oxidized LDL in the absence of cytokines demonstrated cytoplasmic swelling and disruption of the plasma membrane, suggesting cell death by oncosis. Cells exposed to both oxidized LDL and cytokines were characterized by chromatin and cytoplasmic condensation compatible with cell death by apoptosis. These findings further support the notion that oxidized lipids play a role in plaque cell death.}}, author = {{Jovinge, Stefan and Crisby, M and Thyberg, J and Nilsson, J}}, issn = {{1524-4636}}, keywords = {{apoptosis; cell death; oxidized LDL; TNF{alpha}; {gamma}IFN}}, language = {{eng}}, number = {{10}}, pages = {{2225--2231}}, publisher = {{Lippincott Williams & Wilkins}}, series = {{Arteriosclerosis, Thrombosis and Vascular Biology}}, title = {{DNA fragmentation and ultrastructural changes of degenerating cells in atherosclerotic lesions and smooth muscle cells exposed to oxidized LDL in vitro}}, url = {{http://atvb.ahajournals.org/cgi/content/full/17/10/2225}}, volume = {{17}}, year = {{1997}}, }