A novel monoclonal antibody specific for the N-terminal end of GAD65
Hampe, Christiane S ; Lundgren, Per ; Daniels, Terri L ; Hammerle, Lisa P ; Marcovina, Santica M. and Lernmark, Åke LU
(2001)
In Journal of Neuroimmunology
113(1).
p.63-71
- Abstract
We describe a novel monoclonal antibody raised towards the N-terminus of the 65 kDa isoform of glutamate decarboxylase (GAD65). This N-GAD65 mAb is highly specific for GAD65 and can be used in a wide range of applications such as Western blot analysis, immunoprecipitation and immunocytochemistry. Full-length cDNAs coding for N-GAD65 mAb heavy and light chains were cloned and characterized by nucleotide sequence analysis. Both heavy and light chain-specific cDNAs are functional and are members of mouse heavy chain subgroup IgG1 and kappa chain group 2, respectively. Comparing the N-GAD65 mAb gene sequences with GenBank shows that the cDNAs were not reported previously.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/62eb1d35-0ca3-4e9c-9cd9-744833441eb7
- author
- Hampe, Christiane S
; Lundgren, Per
; Daniels, Terri L
; Hammerle, Lisa P
; Marcovina, Santica M.
and Lernmark, Åke
LU
- publishing date
- 2001-02-01
- type
- Contribution to journal
- publication status
- published
- keywords
- Autoimmunity, GAD67, Immunoglobulin, Polymerase chain reaction, Type 1 diabetes
- in
- Journal of Neuroimmunology
- volume
- 113
- issue
- 1
- pages
- 9 pages
- publisher
- Elsevier
- external identifiers
-
- pmid:11137577
- scopus:0035255070
- ISSN
- 0165-5728
- DOI
- 10.1016/S0165-5728(00)00423-9
- language
- English
- LU publication?
- no
- id
- 62eb1d35-0ca3-4e9c-9cd9-744833441eb7
- date added to LUP
- 2017-09-07 09:39:54
- date last changed
- 2024-03-13 08:21:45
@article{62eb1d35-0ca3-4e9c-9cd9-744833441eb7,
abstract = {{<p>We describe a novel monoclonal antibody raised towards the N-terminus of the 65 kDa isoform of glutamate decarboxylase (GAD65). This N-GAD65 mAb is highly specific for GAD65 and can be used in a wide range of applications such as Western blot analysis, immunoprecipitation and immunocytochemistry. Full-length cDNAs coding for N-GAD65 mAb heavy and light chains were cloned and characterized by nucleotide sequence analysis. Both heavy and light chain-specific cDNAs are functional and are members of mouse heavy chain subgroup IgG1 and kappa chain group 2, respectively. Comparing the N-GAD65 mAb gene sequences with GenBank shows that the cDNAs were not reported previously.</p>}},
author = {{Hampe, Christiane S and Lundgren, Per and Daniels, Terri L and Hammerle, Lisa P and Marcovina, Santica M. and Lernmark, Åke}},
issn = {{0165-5728}},
keywords = {{Autoimmunity; GAD67; Immunoglobulin; Polymerase chain reaction; Type 1 diabetes}},
language = {{eng}},
month = {{02}},
number = {{1}},
pages = {{63--71}},
publisher = {{Elsevier}},
series = {{Journal of Neuroimmunology}},
title = {{A novel monoclonal antibody specific for the N-terminal end of GAD65}},
url = {{http://dx.doi.org/10.1016/S0165-5728(00)00423-9}},
doi = {{10.1016/S0165-5728(00)00423-9}},
volume = {{113}},
year = {{2001}},
}