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A novel monoclonal antibody specific for the N-terminal end of GAD65

Hampe, Christiane S; Lundgren, Per; Daniels, Terri L; Hammerle, Lisa P; Marcovina, Santica M. and Lernmark, Åke LU (2001) In Journal of Neuroimmunology 113(1). p.63-71
Abstract

We describe a novel monoclonal antibody raised towards the N-terminus of the 65 kDa isoform of glutamate decarboxylase (GAD65). This N-GAD65 mAb is highly specific for GAD65 and can be used in a wide range of applications such as Western blot analysis, immunoprecipitation and immunocytochemistry. Full-length cDNAs coding for N-GAD65 mAb heavy and light chains were cloned and characterized by nucleotide sequence analysis. Both heavy and light chain-specific cDNAs are functional and are members of mouse heavy chain subgroup IgG1 and kappa chain group 2, respectively. Comparing the N-GAD65 mAb gene sequences with GenBank shows that the cDNAs were not reported previously.

Please use this url to cite or link to this publication:
author
publishing date
type
Contribution to journal
publication status
published
keywords
Autoimmunity, GAD67, Immunoglobulin, Polymerase chain reaction, Type 1 diabetes
in
Journal of Neuroimmunology
volume
113
issue
1
pages
9 pages
publisher
Elsevier
external identifiers
  • scopus:0035255070
ISSN
0165-5728
DOI
10.1016/S0165-5728(00)00423-9
language
English
LU publication?
no
id
62eb1d35-0ca3-4e9c-9cd9-744833441eb7
date added to LUP
2017-09-07 09:39:54
date last changed
2018-01-07 12:18:20
@article{62eb1d35-0ca3-4e9c-9cd9-744833441eb7,
  abstract     = {<p>We describe a novel monoclonal antibody raised towards the N-terminus of the 65 kDa isoform of glutamate decarboxylase (GAD65). This N-GAD65 mAb is highly specific for GAD65 and can be used in a wide range of applications such as Western blot analysis, immunoprecipitation and immunocytochemistry. Full-length cDNAs coding for N-GAD65 mAb heavy and light chains were cloned and characterized by nucleotide sequence analysis. Both heavy and light chain-specific cDNAs are functional and are members of mouse heavy chain subgroup IgG1 and kappa chain group 2, respectively. Comparing the N-GAD65 mAb gene sequences with GenBank shows that the cDNAs were not reported previously.</p>},
  author       = {Hampe, Christiane S and Lundgren, Per and Daniels, Terri L and Hammerle, Lisa P and Marcovina, Santica M. and Lernmark, Åke},
  issn         = {0165-5728},
  keyword      = {Autoimmunity,GAD67,Immunoglobulin,Polymerase chain reaction,Type 1 diabetes},
  language     = {eng},
  month        = {02},
  number       = {1},
  pages        = {63--71},
  publisher    = {Elsevier},
  series       = {Journal of Neuroimmunology},
  title        = {A novel monoclonal antibody specific for the N-terminal end of GAD65},
  url          = {http://dx.doi.org/10.1016/S0165-5728(00)00423-9},
  volume       = {113},
  year         = {2001},
}