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Toward standardization of carbohydrate-deficient transferrin (CDT) measurements: III. Performance of native serum and serum spiked with disialotransferrin proves that harmonization of CDT assays is possible

Weykamp, Cas; Wielders, Jos P. M.; Helander, Anders; Anton, Raymond F.; Bianchi, Vincenza; Jeppsson, Jan-Olof LU ; Siebelder, Carla; Whitfield, John B. and Schellenberg, Francois (2013) In Clinical Chemistry and Laboratory Medicine 51(5). p.991-996
Abstract
Carbohydrate-deficient transferrin (CDT) is a generic term that refers to the transferrin glycoforms whose concentration in blood is temporarily increased by sustained alcohol consumption. Due to high clinical specificity, CDT was proposed as a biomarker of heavy alcohol use and has been available for about 20 years. A number of methods have been developed for CDT measurement based on different analytical techniques and principles and without any harmonization or calibration to a reference method. As a consequence, neither the reference limits nor the cut-off values have been similar across assays, hampering understanding of the diagnostic value of CDT and its routine use. This prompted the International Federation of Clinical Chemistry... (More)
Carbohydrate-deficient transferrin (CDT) is a generic term that refers to the transferrin glycoforms whose concentration in blood is temporarily increased by sustained alcohol consumption. Due to high clinical specificity, CDT was proposed as a biomarker of heavy alcohol use and has been available for about 20 years. A number of methods have been developed for CDT measurement based on different analytical techniques and principles and without any harmonization or calibration to a reference method. As a consequence, neither the reference limits nor the cut-off values have been similar across assays, hampering understanding of the diagnostic value of CDT and its routine use. This prompted the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) to initiate a Working Group on Standardization of CDT (WG-CDT). This third publication of the WG-CDT is devoted to testing the commutability of native and disialotransferrin-spiked serum panels as candidate secondary reference materials, in order to prove the harmonization potential of commercial CDT methods. The results showed that assay harmonization reduced the inter-laboratory imprecision in a network of reference laboratories running the HPLC candidate reference method. In the seven commercial methods evaluated in this study, the use of multi-level secondary calibrators of human serum origin significantly reduced the between-method imprecision. Thus, harmonization of CDT measurements by different methods can be achieved using this calibration system, opening the way for a full standardization of commercial methods against a reference method by use of certified reference materials. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
calibrator, carbohydrate deficient transferrin (CDT), commutability, disialotransferrin, harmonization, HPLC
in
Clinical Chemistry and Laboratory Medicine
volume
51
issue
5
pages
991 - 996
publisher
De Gruyter
external identifiers
  • wos:000318495900021
  • scopus:84882268474
ISSN
1434-6621
DOI
10.1515/cclm-2012-0767
language
English
LU publication?
yes
id
62f6bec7-2d43-4a43-9b60-0f0b8fbbefe0 (old id 3821431)
date added to LUP
2013-07-01 06:59:13
date last changed
2019-02-20 02:04:20
@article{62f6bec7-2d43-4a43-9b60-0f0b8fbbefe0,
  abstract     = {Carbohydrate-deficient transferrin (CDT) is a generic term that refers to the transferrin glycoforms whose concentration in blood is temporarily increased by sustained alcohol consumption. Due to high clinical specificity, CDT was proposed as a biomarker of heavy alcohol use and has been available for about 20 years. A number of methods have been developed for CDT measurement based on different analytical techniques and principles and without any harmonization or calibration to a reference method. As a consequence, neither the reference limits nor the cut-off values have been similar across assays, hampering understanding of the diagnostic value of CDT and its routine use. This prompted the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) to initiate a Working Group on Standardization of CDT (WG-CDT). This third publication of the WG-CDT is devoted to testing the commutability of native and disialotransferrin-spiked serum panels as candidate secondary reference materials, in order to prove the harmonization potential of commercial CDT methods. The results showed that assay harmonization reduced the inter-laboratory imprecision in a network of reference laboratories running the HPLC candidate reference method. In the seven commercial methods evaluated in this study, the use of multi-level secondary calibrators of human serum origin significantly reduced the between-method imprecision. Thus, harmonization of CDT measurements by different methods can be achieved using this calibration system, opening the way for a full standardization of commercial methods against a reference method by use of certified reference materials.},
  author       = {Weykamp, Cas and Wielders, Jos P. M. and Helander, Anders and Anton, Raymond F. and Bianchi, Vincenza and Jeppsson, Jan-Olof and Siebelder, Carla and Whitfield, John B. and Schellenberg, Francois},
  issn         = {1434-6621},
  keyword      = {calibrator,carbohydrate deficient transferrin (CDT),commutability,disialotransferrin,harmonization,HPLC},
  language     = {eng},
  number       = {5},
  pages        = {991--996},
  publisher    = {De Gruyter},
  series       = {Clinical Chemistry and Laboratory Medicine},
  title        = {Toward standardization of carbohydrate-deficient transferrin (CDT) measurements: III. Performance of native serum and serum spiked with disialotransferrin proves that harmonization of CDT assays is possible},
  url          = {http://dx.doi.org/10.1515/cclm-2012-0767},
  volume       = {51},
  year         = {2013},
}