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An Improvement on MS-based Epigenetic Analysis of Large Histone-derived Peptides by Using the IVU Interface.

Fukuda, Tetsuya ; Hike, Hiroshi ; Usui, Fumihiko ; Bando, Yasuhiko ; Nishimura, Toshide LU ; Kodama, Tatsuhiko and Kawamura, Takeshi (2015) In Analytical Biochemistry 486(Online 05 June 2015). p.14-16
Abstract
Highly protonated histone-derived peptides impede a sufficient mass spectrometry (MS) -based epigenetic analysis since their relatively low m/z due to a high degree of proton addition to peptides would difficult to analyze the resulting complex MS/MS spectra. In order to reduce the degree of protonations, we have developed a new interface, the IVU, in which peptides are ionized under a vaporized organic solvent, and it has demonstrated that the doubly-charged histone-tail H2B peptide, PEPAKSAPAPKKGSKKAVTKAQKK (m/z 1238.243 , +2), has been detected by using the IVU interface and sequenced, which was not detectable before.
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author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytical Biochemistry
volume
486
issue
Online 05 June 2015
pages
14 - 16
publisher
Elsevier
external identifiers
  • pmid:26050628
  • wos:000359881000003
  • scopus:84937551726
  • pmid:26050628
ISSN
1096-0309
DOI
10.1016/j.ab.2015.06.002
language
English
LU publication?
yes
id
635dfa16-c94f-4228-9e80-39735122aea3 (old id 7487715)
date added to LUP
2016-04-01 10:47:41
date last changed
2025-10-14 12:34:47
@article{635dfa16-c94f-4228-9e80-39735122aea3,
  abstract     = {{Highly protonated histone-derived peptides impede a sufficient mass spectrometry (MS) -based epigenetic analysis since their relatively low m/z due to a high degree of proton addition to peptides would difficult to analyze the resulting complex MS/MS spectra. In order to reduce the degree of protonations, we have developed a new interface, the IVU, in which peptides are ionized under a vaporized organic solvent, and it has demonstrated that the doubly-charged histone-tail H2B peptide, PEPAKSAPAPKKGSKKAVTKAQKK (m/z 1238.243 , +2), has been detected by using the IVU interface and sequenced, which was not detectable before.}},
  author       = {{Fukuda, Tetsuya and Hike, Hiroshi and Usui, Fumihiko and Bando, Yasuhiko and Nishimura, Toshide and Kodama, Tatsuhiko and Kawamura, Takeshi}},
  issn         = {{1096-0309}},
  language     = {{eng}},
  number       = {{Online 05 June 2015}},
  pages        = {{14--16}},
  publisher    = {{Elsevier}},
  series       = {{Analytical Biochemistry}},
  title        = {{An Improvement on MS-based Epigenetic Analysis of Large Histone-derived Peptides by Using the IVU Interface.}},
  url          = {{http://dx.doi.org/10.1016/j.ab.2015.06.002}},
  doi          = {{10.1016/j.ab.2015.06.002}},
  volume       = {{486}},
  year         = {{2015}},
}