Accessing microenvironment compartments in formalin-fixed paraffin-embedded tissues by protein expression analysis.
Donnarumma, Fabrizio LU ; Végvári, Ákos LU ; Rezeli, Melinda LU
; Welinder, Charlotte
LU
; Jansson, Bo
LU
and Marko-Varga, György
LU
(2013)
In Bioanalysis
5(21).
p.2647-2659
- Abstract
- Background: Formalin-fixed paraffin-embedded (FFPE) samples are an outstanding source of new information regarding disease evolvements. Current research on new biomarkers and diseases features has recently invested resources in FFPE-related projects. Results: In order to initiate clinical protein-expression studies using minute amount of biological material, a workflow based on the combination of filter-assisted sample preparation with MS analysis and label-free quantification was developed. Xenograft lung tumor tissue was investigated as a model system. The workflow was optimized and characterized in terms of its reproducibility from a quantitative and qualitative point of view. We proposed a modification of the original filter-assisted... (More)
- Background: Formalin-fixed paraffin-embedded (FFPE) samples are an outstanding source of new information regarding disease evolvements. Current research on new biomarkers and diseases features has recently invested resources in FFPE-related projects. Results: In order to initiate clinical protein-expression studies using minute amount of biological material, a workflow based on the combination of filter-assisted sample preparation with MS analysis and label-free quantification was developed. Xenograft lung tumor tissue was investigated as a model system. The workflow was optimized and characterized in terms of its reproducibility from a quantitative and qualitative point of view. We proposed a modification of the original filter-assisted sample preparation protocol to improve reproducibility and highlight its potential for the investigation of hydrophobic proteins. Conclusions: Altogether the presented workflow allows analysis of FFPE samples with improvements in the analytical time and performance, and we show its application for lung cancer xenograft tissue samples. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4179906
- author
- Donnarumma, Fabrizio
LU
; Végvári, Ákos
LU
; Rezeli, Melinda
LU
; Welinder, Charlotte
LU
; Jansson, Bo
LU
and Marko-Varga, György
LU
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Bioanalysis
- volume
- 5
- issue
- 21
- pages
- 2647 - 2659
- publisher
- Future Science
- external identifiers
-
- wos:000326449000011
- pmid:24180505
- scopus:84904532273
- pmid:24180505
- ISSN
- 1757-6180
- DOI
- 10.4155/bio.13.222
- language
- English
- LU publication?
- yes
- id
- 63b3df8d-f2ce-472a-a5d0-30901a11c5bf (old id 4179906)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/24180505?dopt=Abstract
- date added to LUP
- 2016-04-01 09:56:31
- date last changed
- 2023-08-30 13:44:48
@article{63b3df8d-f2ce-472a-a5d0-30901a11c5bf,
abstract = {{Background: Formalin-fixed paraffin-embedded (FFPE) samples are an outstanding source of new information regarding disease evolvements. Current research on new biomarkers and diseases features has recently invested resources in FFPE-related projects. Results: In order to initiate clinical protein-expression studies using minute amount of biological material, a workflow based on the combination of filter-assisted sample preparation with MS analysis and label-free quantification was developed. Xenograft lung tumor tissue was investigated as a model system. The workflow was optimized and characterized in terms of its reproducibility from a quantitative and qualitative point of view. We proposed a modification of the original filter-assisted sample preparation protocol to improve reproducibility and highlight its potential for the investigation of hydrophobic proteins. Conclusions: Altogether the presented workflow allows analysis of FFPE samples with improvements in the analytical time and performance, and we show its application for lung cancer xenograft tissue samples.}},
author = {{Donnarumma, Fabrizio and Végvári, Ákos and Rezeli, Melinda and Welinder, Charlotte and Jansson, Bo and Marko-Varga, György}},
issn = {{1757-6180}},
language = {{eng}},
number = {{21}},
pages = {{2647--2659}},
publisher = {{Future Science}},
series = {{Bioanalysis}},
title = {{Accessing microenvironment compartments in formalin-fixed paraffin-embedded tissues by protein expression analysis.}},
url = {{http://dx.doi.org/10.4155/bio.13.222}},
doi = {{10.4155/bio.13.222}},
volume = {{5}},
year = {{2013}},
}