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Production of Cystatin C Wild Type and Stabilized Mutants

Pollak, Joanna LU ; Szymanska, Aneta; Lindström, Veronica LU and Grubb, Anders LU (2010) In EJIFCC 20(4). p.70-166
Abstract

Cystatin C is produced in all nucleated cells. It has various functions and biological activities. Researchers are focused on its role in kidney diseases as a marker of glomerular filtration but also as a very important link in development of amyloid diseases. This work describes expression and purification of both wild type (wt) and stabilized form (stab 1 and 2) of wt cystatin C and amyloid-forming L68Q mutant of cystatin C. The recombinant cystatin C can be used in projects requiring pure cystatin C to examine models of dimerization and fibrils formation as well as a standard in clinical tests.

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organization
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Contribution to journal
publication status
published
subject
keywords
Journal Article
in
EJIFCC
volume
20
issue
4
pages
5 pages
ISSN
1650-3414
language
English
LU publication?
yes
id
640ac852-1071-4ed3-b7de-ccfdca16f689
date added to LUP
2016-11-22 19:02:22
date last changed
2018-05-29 11:06:17
@article{640ac852-1071-4ed3-b7de-ccfdca16f689,
  abstract     = {<p>Cystatin C is produced in all nucleated cells. It has various functions and biological activities. Researchers are focused on its role in kidney diseases as a marker of glomerular filtration but also as a very important link in development of amyloid diseases. This work describes expression and purification of both wild type (wt) and stabilized form (stab 1 and 2) of wt cystatin C and amyloid-forming L68Q mutant of cystatin C. The recombinant cystatin C can be used in projects requiring pure cystatin C to examine models of dimerization and fibrils formation as well as a standard in clinical tests.</p>},
  author       = {Pollak, Joanna and Szymanska, Aneta and Lindström, Veronica and Grubb, Anders},
  issn         = {1650-3414},
  keyword      = {Journal Article},
  language     = {eng},
  number       = {4},
  pages        = {70--166},
  series       = {EJIFCC},
  title        = {Production of Cystatin C Wild Type and Stabilized Mutants},
  volume       = {20},
  year         = {2010},
}