Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Production of Cystatin C Wild Type and Stabilized Mutants

Pollak, Joanna LU ; Szymanska, Aneta ; Lindström, Veronica LU orcid and Grubb, Anders LU orcid (2010) In EJIFCC 20(4). p.70-166
Abstract

Cystatin C is produced in all nucleated cells. It has various functions and biological activities. Researchers are focused on its role in kidney diseases as a marker of glomerular filtration but also as a very important link in development of amyloid diseases. This work describes expression and purification of both wild type (wt) and stabilized form (stab 1 and 2) of wt cystatin C and amyloid-forming L68Q mutant of cystatin C. The recombinant cystatin C can be used in projects requiring pure cystatin C to examine models of dimerization and fibrils formation as well as a standard in clinical tests.

Please use this url to cite or link to this publication:
author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Journal Article
in
EJIFCC
volume
20
issue
4
pages
5 pages
publisher
International Federation of Clinical Chemistry and Laboratory Medicine
external identifiers
  • pmid:27683345
ISSN
1650-3414
language
English
LU publication?
yes
id
640ac852-1071-4ed3-b7de-ccfdca16f689
date added to LUP
2016-11-22 19:02:22
date last changed
2025-04-04 14:47:44
@article{640ac852-1071-4ed3-b7de-ccfdca16f689,
  abstract     = {{<p>Cystatin C is produced in all nucleated cells. It has various functions and biological activities. Researchers are focused on its role in kidney diseases as a marker of glomerular filtration but also as a very important link in development of amyloid diseases. This work describes expression and purification of both wild type (wt) and stabilized form (stab 1 and 2) of wt cystatin C and amyloid-forming L68Q mutant of cystatin C. The recombinant cystatin C can be used in projects requiring pure cystatin C to examine models of dimerization and fibrils formation as well as a standard in clinical tests.</p>}},
  author       = {{Pollak, Joanna and Szymanska, Aneta and Lindström, Veronica and Grubb, Anders}},
  issn         = {{1650-3414}},
  keywords     = {{Journal Article}},
  language     = {{eng}},
  number       = {{4}},
  pages        = {{70--166}},
  publisher    = {{International Federation of Clinical Chemistry and Laboratory Medicine}},
  series       = {{EJIFCC}},
  title        = {{Production of Cystatin C Wild Type and Stabilized Mutants}},
  volume       = {{20}},
  year         = {{2010}},
}