Analysis of vesicular trafficking in primary neurons by live imaging.
Tampellini, Davide LU and Gouras, Gunnar LU
(2011)
In Methods in Molecular Biology
793.
p.343-350
- Abstract
- Alzheimer's disease, the most common neurodegenerative disease, is characterized by a progressive loss of synapses and accumulation of amyloid-beta (Aβ) peptides in the brain. Previous studies demonstrated that acute increase in synaptic activity in cultured hippocampal slices and mouse brains (Cirrito et al. Neuron 48: 913-922, 2005; Kamenetz et al. Neuron 37: 925-937, 2003) enhanced secretion of Aβ. Since synaptic activity promotes Aβ secretion, it could also affect the trafficking and processing of its precursor, the amyloid precursor protein (APP). Here, we describe a method to investigate the effect of acute synaptic activation on APP trafficking within dendrites.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2168939
- author
- Tampellini, Davide
LU
and Gouras, Gunnar
LU
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Methods in Molecular Biology
- volume
- 793
- pages
- 343 - 350
- publisher
- Springer
- external identifiers
-
- pmid:21913111
- scopus:80054751590
- ISSN
- 1940-6029
- DOI
- 10.1007/978-1-61779-328-8_22
- language
- English
- LU publication?
- yes
- id
- 641b8a78-22c6-4150-866c-4bf065961436 (old id 2168939)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/21913111?dopt=Abstract
- date added to LUP
- 2016-04-04 08:56:07
- date last changed
- 2022-01-29 07:50:28
@article{641b8a78-22c6-4150-866c-4bf065961436,
abstract = {{Alzheimer's disease, the most common neurodegenerative disease, is characterized by a progressive loss of synapses and accumulation of amyloid-beta (Aβ) peptides in the brain. Previous studies demonstrated that acute increase in synaptic activity in cultured hippocampal slices and mouse brains (Cirrito et al. Neuron 48: 913-922, 2005; Kamenetz et al. Neuron 37: 925-937, 2003) enhanced secretion of Aβ. Since synaptic activity promotes Aβ secretion, it could also affect the trafficking and processing of its precursor, the amyloid precursor protein (APP). Here, we describe a method to investigate the effect of acute synaptic activation on APP trafficking within dendrites.}},
author = {{Tampellini, Davide and Gouras, Gunnar}},
issn = {{1940-6029}},
language = {{eng}},
pages = {{343--350}},
publisher = {{Springer}},
series = {{Methods in Molecular Biology}},
title = {{Analysis of vesicular trafficking in primary neurons by live imaging.}},
url = {{http://dx.doi.org/10.1007/978-1-61779-328-8_22}},
doi = {{10.1007/978-1-61779-328-8_22}},
volume = {{793}},
year = {{2011}},
}