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Superresolution microscopy of the volume phase transition of pNIPAM microgels

Conley, Gaurasundar M.; Nöjd, Sofi LU ; Braibanti, Marco; Schurtenberger, Peter LU and Scheffold, Frank (2016) In Colloids and Surfaces A: Physicochemical and Engineering Aspects 499. p.18-23
Abstract

Hierarchical polymer structures such as pNIPAM microgels have been extensively studied for their ability to undergo structural and physical transformations that can be controlled by external stimuli such as temperature, pH or solvent composition. However, a direct three-dimensional visualization of individual particles in-situ has so far been hindered by insufficient resolution, with optical microscopy, or contrast, with electron microscopy. In recent years superresolution microscopy techniques have emerged that can provide nanoscopic optical resolution. Here we report on the in-situ superresolution microscopy of dye-labelled submicron sized pNIPAM microgels revealing the internal density profile during swelling and collapse of... (More)

Hierarchical polymer structures such as pNIPAM microgels have been extensively studied for their ability to undergo structural and physical transformations that can be controlled by external stimuli such as temperature, pH or solvent composition. However, a direct three-dimensional visualization of individual particles in-situ has so far been hindered by insufficient resolution, with optical microscopy, or contrast, with electron microscopy. In recent years superresolution microscopy techniques have emerged that can provide nanoscopic optical resolution. Here we report on the in-situ superresolution microscopy of dye-labelled submicron sized pNIPAM microgels revealing the internal density profile during swelling and collapse of individual particles. Using direct STochastic Optical Reconstruction Microscopy (dSTORM) we demonstrate a lateral optical resolution of 30 nm and an axial resolution of 60 nm.

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Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
DSTORM, Microgels, PNIPAM, Super resolution microscopy
in
Colloids and Surfaces A: Physicochemical and Engineering Aspects
volume
499
pages
6 pages
publisher
Elsevier
external identifiers
  • scopus:84962731736
  • wos:000375859800003
ISSN
0927-7757
DOI
10.1016/j.colsurfa.2016.03.010
language
English
LU publication?
yes
id
64305ae0-78b0-425a-bdf4-fba6e356c836
date added to LUP
2016-04-29 12:08:56
date last changed
2017-03-12 04:33:08
@article{64305ae0-78b0-425a-bdf4-fba6e356c836,
  abstract     = {<p>Hierarchical polymer structures such as pNIPAM microgels have been extensively studied for their ability to undergo structural and physical transformations that can be controlled by external stimuli such as temperature, pH or solvent composition. However, a direct three-dimensional visualization of individual particles in-situ has so far been hindered by insufficient resolution, with optical microscopy, or contrast, with electron microscopy. In recent years superresolution microscopy techniques have emerged that can provide nanoscopic optical resolution. Here we report on the in-situ superresolution microscopy of dye-labelled submicron sized pNIPAM microgels revealing the internal density profile during swelling and collapse of individual particles. Using direct STochastic Optical Reconstruction Microscopy (dSTORM) we demonstrate a lateral optical resolution of 30 nm and an axial resolution of 60 nm.</p>},
  author       = {Conley, Gaurasundar M. and Nöjd, Sofi and Braibanti, Marco and Schurtenberger, Peter and Scheffold, Frank},
  issn         = {0927-7757},
  keyword      = {DSTORM,Microgels,PNIPAM,Super resolution microscopy},
  language     = {eng},
  month        = {06},
  pages        = {18--23},
  publisher    = {Elsevier},
  series       = {Colloids and Surfaces A: Physicochemical and Engineering Aspects},
  title        = {Superresolution microscopy of the volume phase transition of pNIPAM microgels},
  url          = {http://dx.doi.org/10.1016/j.colsurfa.2016.03.010},
  volume       = {499},
  year         = {2016},
}