Comparison of two glutathione S-transferases used in capacitive biosensors for detection of heavy metals
(2007) In International Journal of Environmental Analytical Chemistry 87(10-11). p.745-754- Abstract
- This work describes the development of a heavy-metal biosensor based on either recombinant 6His-Tag glutathione S-transferase (GST-(His)(6)) or glutathione S-transferase Theta 2-2 (GST-theta 2-2), and a capacitive transducer. The dynamic range of the pure bovine liver GST-Theta 2-2 biosensor was 1 fM to 1mM for Zn2+, and 10pM to 1mM for Cd2+. The GST-(His) 6 biosensor was able to detect Zn2+ and Cd2+ in the range of 1 fM to 10 mu M, and Hg2+ in the range of 1 fM to 10mM. The bovine liver GST Theta 2-2 biosensor displays an increased selectivity and a wider dynamic range for Zn2+ compared with the GST-(His) 6 biosensor. Therefore, by using different GST isozymes, it is possible to modulate important characteristics of capacitive biosensors... (More)
- This work describes the development of a heavy-metal biosensor based on either recombinant 6His-Tag glutathione S-transferase (GST-(His)(6)) or glutathione S-transferase Theta 2-2 (GST-theta 2-2), and a capacitive transducer. The dynamic range of the pure bovine liver GST-Theta 2-2 biosensor was 1 fM to 1mM for Zn2+, and 10pM to 1mM for Cd2+. The GST-(His) 6 biosensor was able to detect Zn2+ and Cd2+ in the range of 1 fM to 10 mu M, and Hg2+ in the range of 1 fM to 10mM. The bovine liver GST Theta 2-2 biosensor displays an increased selectivity and a wider dynamic range for Zn2+ compared with the GST-(His) 6 biosensor. Therefore, by using different GST isozymes, it is possible to modulate important characteristics of capacitive biosensors for the detection of heavy metals. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/645719
- author
- Saatci, Ebru LU ; Nistor, Mihaela LU ; Gaspar, Szilveszter LU ; Csöregi, Elisabeth LU and Iscan, Mesude
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- bovine liver, capacitive biosensor, 6His-tag glutathione S-transferase, glutathione S-tranferase theta 2-2, heavy-metal detection
- in
- International Journal of Environmental Analytical Chemistry
- volume
- 87
- issue
- 10-11
- pages
- 745 - 754
- publisher
- Taylor & Francis
- external identifiers
-
- wos:000248051600007
- scopus:34547164474
- ISSN
- 1029-0397
- DOI
- 10.1080/03067310701409309
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
- id
- 78509e97-3568-4b78-9585-dadcde54fbc6 (old id 645719)
- date added to LUP
- 2016-04-01 12:12:10
- date last changed
- 2022-04-29 02:07:00
@article{78509e97-3568-4b78-9585-dadcde54fbc6, abstract = {{This work describes the development of a heavy-metal biosensor based on either recombinant 6His-Tag glutathione S-transferase (GST-(His)(6)) or glutathione S-transferase Theta 2-2 (GST-theta 2-2), and a capacitive transducer. The dynamic range of the pure bovine liver GST-Theta 2-2 biosensor was 1 fM to 1mM for Zn2+, and 10pM to 1mM for Cd2+. The GST-(His) 6 biosensor was able to detect Zn2+ and Cd2+ in the range of 1 fM to 10 mu M, and Hg2+ in the range of 1 fM to 10mM. The bovine liver GST Theta 2-2 biosensor displays an increased selectivity and a wider dynamic range for Zn2+ compared with the GST-(His) 6 biosensor. Therefore, by using different GST isozymes, it is possible to modulate important characteristics of capacitive biosensors for the detection of heavy metals.}}, author = {{Saatci, Ebru and Nistor, Mihaela and Gaspar, Szilveszter and Csöregi, Elisabeth and Iscan, Mesude}}, issn = {{1029-0397}}, keywords = {{bovine liver; capacitive biosensor; 6His-tag glutathione S-transferase; glutathione S-tranferase theta 2-2; heavy-metal detection}}, language = {{eng}}, number = {{10-11}}, pages = {{745--754}}, publisher = {{Taylor & Francis}}, series = {{International Journal of Environmental Analytical Chemistry}}, title = {{Comparison of two glutathione S-transferases used in capacitive biosensors for detection of heavy metals}}, url = {{http://dx.doi.org/10.1080/03067310701409309}}, doi = {{10.1080/03067310701409309}}, volume = {{87}}, year = {{2007}}, }