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Comparison of two glutathione S-transferases used in capacitive biosensors for detection of heavy metals

Saatci, Ebru LU ; Nistor, Mihaela LU ; Gaspar, Szilveszter LU ; Csöregi, Elisabeth LU and Iscan, Mesude (2007) In International Journal of Environmental Analytical Chemistry 87(10-11). p.745-754
Abstract
This work describes the development of a heavy-metal biosensor based on either recombinant 6His-Tag glutathione S-transferase (GST-(His)(6)) or glutathione S-transferase Theta 2-2 (GST-theta 2-2), and a capacitive transducer. The dynamic range of the pure bovine liver GST-Theta 2-2 biosensor was 1 fM to 1mM for Zn2+, and 10pM to 1mM for Cd2+. The GST-(His) 6 biosensor was able to detect Zn2+ and Cd2+ in the range of 1 fM to 10 mu M, and Hg2+ in the range of 1 fM to 10mM. The bovine liver GST Theta 2-2 biosensor displays an increased selectivity and a wider dynamic range for Zn2+ compared with the GST-(His) 6 biosensor. Therefore, by using different GST isozymes, it is possible to modulate important characteristics of capacitive biosensors... (More)
This work describes the development of a heavy-metal biosensor based on either recombinant 6His-Tag glutathione S-transferase (GST-(His)(6)) or glutathione S-transferase Theta 2-2 (GST-theta 2-2), and a capacitive transducer. The dynamic range of the pure bovine liver GST-Theta 2-2 biosensor was 1 fM to 1mM for Zn2+, and 10pM to 1mM for Cd2+. The GST-(His) 6 biosensor was able to detect Zn2+ and Cd2+ in the range of 1 fM to 10 mu M, and Hg2+ in the range of 1 fM to 10mM. The bovine liver GST Theta 2-2 biosensor displays an increased selectivity and a wider dynamic range for Zn2+ compared with the GST-(His) 6 biosensor. Therefore, by using different GST isozymes, it is possible to modulate important characteristics of capacitive biosensors for the detection of heavy metals. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
bovine liver, capacitive biosensor, 6His-tag glutathione S-transferase, glutathione S-tranferase theta 2-2, heavy-metal detection
in
International Journal of Environmental Analytical Chemistry
volume
87
issue
10-11
pages
745 - 754
publisher
Taylor & Francis
external identifiers
  • wos:000248051600007
  • scopus:34547164474
ISSN
1029-0397
DOI
10.1080/03067310701409309
language
English
LU publication?
yes
id
78509e97-3568-4b78-9585-dadcde54fbc6 (old id 645719)
date added to LUP
2007-12-21 14:41:13
date last changed
2017-01-01 04:56:00
@article{78509e97-3568-4b78-9585-dadcde54fbc6,
  abstract     = {This work describes the development of a heavy-metal biosensor based on either recombinant 6His-Tag glutathione S-transferase (GST-(His)(6)) or glutathione S-transferase Theta 2-2 (GST-theta 2-2), and a capacitive transducer. The dynamic range of the pure bovine liver GST-Theta 2-2 biosensor was 1 fM to 1mM for Zn2+, and 10pM to 1mM for Cd2+. The GST-(His) 6 biosensor was able to detect Zn2+ and Cd2+ in the range of 1 fM to 10 mu M, and Hg2+ in the range of 1 fM to 10mM. The bovine liver GST Theta 2-2 biosensor displays an increased selectivity and a wider dynamic range for Zn2+ compared with the GST-(His) 6 biosensor. Therefore, by using different GST isozymes, it is possible to modulate important characteristics of capacitive biosensors for the detection of heavy metals.},
  author       = {Saatci, Ebru and Nistor, Mihaela and Gaspar, Szilveszter and Csöregi, Elisabeth and Iscan, Mesude},
  issn         = {1029-0397},
  keyword      = {bovine liver,capacitive biosensor,6His-tag glutathione S-transferase,glutathione S-tranferase theta 2-2,heavy-metal detection},
  language     = {eng},
  number       = {10-11},
  pages        = {745--754},
  publisher    = {Taylor & Francis},
  series       = {International Journal of Environmental Analytical Chemistry},
  title        = {Comparison of two glutathione S-transferases used in capacitive biosensors for detection of heavy metals},
  url          = {http://dx.doi.org/10.1080/03067310701409309},
  volume       = {87},
  year         = {2007},
}