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The Wilms' tumor gene 1 (WT1) induces expression of the N-myc downstream regulated gene 2 (NDRG2)

Svensson, Emelie LU ; Vidovic, Karina LU ; Olofsson, Tor LU ; Vallon-Christersson, Johan LU ; Borg, Åke LU and Gullberg, Urban LU (2007) In DNA and Cell Biology 26(8). p.589-597
Abstract
The Wilms' tumor gene 1 (WT1) protein is a transcriptional regulator that is highly expressed in immature hematopoietic progenitor cells and in the majority of patients with acute and chronic myeloid leukemia. However, it is still unclear how WT1 exerts its function(s) in hematopoietic cells. The aim of this work was to investigate the function of WT1 as a transcription factor in human hematopoietic progenitor cells. To this end, an oligonucleotide array approach was used to study the gene expression in CD34(+) cells from human cord blood retrovirally transduced with WT1 or a control vector. We found that the expression of the putative tumor suppressor gene N-myc downstream regulated gene 2 (NDRG2) mRNA was induced by WT1 in CD34(+) cells... (More)
The Wilms' tumor gene 1 (WT1) protein is a transcriptional regulator that is highly expressed in immature hematopoietic progenitor cells and in the majority of patients with acute and chronic myeloid leukemia. However, it is still unclear how WT1 exerts its function(s) in hematopoietic cells. The aim of this work was to investigate the function of WT1 as a transcription factor in human hematopoietic progenitor cells. To this end, an oligonucleotide array approach was used to study the gene expression in CD34(+) cells from human cord blood retrovirally transduced with WT1 or a control vector. We found that the expression of the putative tumor suppressor gene N-myc downstream regulated gene 2 (NDRG2) mRNA was induced by WT1 in CD34(+) cells and also in leukemic U937 cells. Furthermore, a novel transcription start site in the NDRG2 gene was identified in WT1-transduced cells, in addition to two previously reported transcription start sites. These results show that the expression of the NDRG2 gene is directly or indirectly induced by WT1, and provide the first insights into transcriptional regulation of the NDRG2 gene, including demonstration of a novel splice variant. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
DNA and Cell Biology
volume
26
issue
8
pages
589 - 597
publisher
Mary Ann Liebert, Inc.
external identifiers
  • wos:000248656900008
  • scopus:34547899857
ISSN
1044-5498
DOI
10.1089/dna.2007.0586
project
CREATE Health
language
English
LU publication?
yes
id
2297b917-6555-4bb3-a37b-7b9673a18c57 (old id 645826)
date added to LUP
2007-12-18 15:55:52
date last changed
2017-07-23 04:34:10
@article{2297b917-6555-4bb3-a37b-7b9673a18c57,
  abstract     = {The Wilms' tumor gene 1 (WT1) protein is a transcriptional regulator that is highly expressed in immature hematopoietic progenitor cells and in the majority of patients with acute and chronic myeloid leukemia. However, it is still unclear how WT1 exerts its function(s) in hematopoietic cells. The aim of this work was to investigate the function of WT1 as a transcription factor in human hematopoietic progenitor cells. To this end, an oligonucleotide array approach was used to study the gene expression in CD34(+) cells from human cord blood retrovirally transduced with WT1 or a control vector. We found that the expression of the putative tumor suppressor gene N-myc downstream regulated gene 2 (NDRG2) mRNA was induced by WT1 in CD34(+) cells and also in leukemic U937 cells. Furthermore, a novel transcription start site in the NDRG2 gene was identified in WT1-transduced cells, in addition to two previously reported transcription start sites. These results show that the expression of the NDRG2 gene is directly or indirectly induced by WT1, and provide the first insights into transcriptional regulation of the NDRG2 gene, including demonstration of a novel splice variant.},
  author       = {Svensson, Emelie and Vidovic, Karina and Olofsson, Tor and Vallon-Christersson, Johan and Borg, Åke and Gullberg, Urban},
  issn         = {1044-5498},
  language     = {eng},
  number       = {8},
  pages        = {589--597},
  publisher    = {Mary Ann Liebert, Inc.},
  series       = {DNA and Cell Biology},
  title        = {The Wilms' tumor gene 1 (WT1) induces expression of the N-myc downstream regulated gene 2 (NDRG2)},
  url          = {http://dx.doi.org/10.1089/dna.2007.0586},
  volume       = {26},
  year         = {2007},
}