The Wilms' tumor gene 1 (WT1) induces expression of the N-myc downstream regulated gene 2 (NDRG2)
Svensson, Emelie LU ; Vidovic, Karina LU ; Olofsson, Tor LU ; Vallon-Christersson, Johan LU
; Borg, Åke
LU
and Gullberg, Urban
LU
(2007)
In DNA and Cell Biology
26(8).
p.589-597
- Abstract
- The Wilms' tumor gene 1 (WT1) protein is a transcriptional regulator that is highly expressed in immature hematopoietic progenitor cells and in the majority of patients with acute and chronic myeloid leukemia. However, it is still unclear how WT1 exerts its function(s) in hematopoietic cells. The aim of this work was to investigate the function of WT1 as a transcription factor in human hematopoietic progenitor cells. To this end, an oligonucleotide array approach was used to study the gene expression in CD34(+) cells from human cord blood retrovirally transduced with WT1 or a control vector. We found that the expression of the putative tumor suppressor gene N-myc downstream regulated gene 2 (NDRG2) mRNA was induced by WT1 in CD34(+) cells... (More)
- The Wilms' tumor gene 1 (WT1) protein is a transcriptional regulator that is highly expressed in immature hematopoietic progenitor cells and in the majority of patients with acute and chronic myeloid leukemia. However, it is still unclear how WT1 exerts its function(s) in hematopoietic cells. The aim of this work was to investigate the function of WT1 as a transcription factor in human hematopoietic progenitor cells. To this end, an oligonucleotide array approach was used to study the gene expression in CD34(+) cells from human cord blood retrovirally transduced with WT1 or a control vector. We found that the expression of the putative tumor suppressor gene N-myc downstream regulated gene 2 (NDRG2) mRNA was induced by WT1 in CD34(+) cells and also in leukemic U937 cells. Furthermore, a novel transcription start site in the NDRG2 gene was identified in WT1-transduced cells, in addition to two previously reported transcription start sites. These results show that the expression of the NDRG2 gene is directly or indirectly induced by WT1, and provide the first insights into transcriptional regulation of the NDRG2 gene, including demonstration of a novel splice variant. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/645826
- author
- Svensson, Emelie
LU
; Vidovic, Karina
LU
; Olofsson, Tor
LU
; Vallon-Christersson, Johan
LU
; Borg, Åke
LU
and Gullberg, Urban
LU
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- in
- DNA and Cell Biology
- volume
- 26
- issue
- 8
- pages
- 589 - 597
- publisher
- Mary Ann Liebert, Inc.
- external identifiers
-
- wos:000248656900008
- scopus:34547899857
- ISSN
- 1044-5498
- DOI
- 10.1089/dna.2007.0586
- language
- English
- LU publication?
- yes
- id
- 2297b917-6555-4bb3-a37b-7b9673a18c57 (old id 645826)
- date added to LUP
- 2016-04-01 15:48:39
- date last changed
- 2024-01-10 20:12:01
@article{2297b917-6555-4bb3-a37b-7b9673a18c57,
abstract = {{The Wilms' tumor gene 1 (WT1) protein is a transcriptional regulator that is highly expressed in immature hematopoietic progenitor cells and in the majority of patients with acute and chronic myeloid leukemia. However, it is still unclear how WT1 exerts its function(s) in hematopoietic cells. The aim of this work was to investigate the function of WT1 as a transcription factor in human hematopoietic progenitor cells. To this end, an oligonucleotide array approach was used to study the gene expression in CD34(+) cells from human cord blood retrovirally transduced with WT1 or a control vector. We found that the expression of the putative tumor suppressor gene N-myc downstream regulated gene 2 (NDRG2) mRNA was induced by WT1 in CD34(+) cells and also in leukemic U937 cells. Furthermore, a novel transcription start site in the NDRG2 gene was identified in WT1-transduced cells, in addition to two previously reported transcription start sites. These results show that the expression of the NDRG2 gene is directly or indirectly induced by WT1, and provide the first insights into transcriptional regulation of the NDRG2 gene, including demonstration of a novel splice variant.}},
author = {{Svensson, Emelie and Vidovic, Karina and Olofsson, Tor and Vallon-Christersson, Johan and Borg, Åke and Gullberg, Urban}},
issn = {{1044-5498}},
language = {{eng}},
number = {{8}},
pages = {{589--597}},
publisher = {{Mary Ann Liebert, Inc.}},
series = {{DNA and Cell Biology}},
title = {{The Wilms' tumor gene 1 (WT1) induces expression of the N-myc downstream regulated gene 2 (NDRG2)}},
url = {{http://dx.doi.org/10.1089/dna.2007.0586}},
doi = {{10.1089/dna.2007.0586}},
volume = {{26}},
year = {{2007}},
}