Advanced

Validation of the use of Pap-stained vaginal smears for diagnosis of bacterial vaginosis

Eriksson, K.; Forsum, U.; Bjornerem, A.; Platz-Christensen, Jens Jörgen LU and Larsson, P. G. (2007) In Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS) 115(7). p.809-813
Abstract
Papanicolaou-stained cervicovaginal smears (Pap smears) are used to screen for cervical cancer. Since there is a lack of consensus in published reports respecting the efficacy of Pap-stained smears in BV diagnostics, there is a need to validate their use for diagnosis of BV. Slides from the international BV00 workshop were Pap stained and independently analyzed by four investigators under a phase-contrast microscope. All workshop slides-whether Pap-stained, Gram-stained or rehydrated air-dried smears-were scored according to the same Nugent classification. The diagnostic accuracy of Pap smears for diagnosis of BV had a sensitivity of 0.85 and a specificity of 0.92, with a positive and negative predictive value of 0.84 and 0.93,... (More)
Papanicolaou-stained cervicovaginal smears (Pap smears) are used to screen for cervical cancer. Since there is a lack of consensus in published reports respecting the efficacy of Pap-stained smears in BV diagnostics, there is a need to validate their use for diagnosis of BV. Slides from the international BV00 workshop were Pap stained and independently analyzed by four investigators under a phase-contrast microscope. All workshop slides-whether Pap-stained, Gram-stained or rehydrated air-dried smears-were scored according to the same Nugent classification. The diagnostic accuracy of Pap smears for diagnosis of BV had a sensitivity of 0.85 and a specificity of 0.92, with a positive and negative predictive value of 0.84 and 0.93, respectively. The interobserver weighted kappa index was 0.86 for Pap-stained smears compared to 0.81 for Gram-stained smears, and 0.70 for rehydrated air-dried smears using the mean Nugent score as the criterion standard. Provided that the samples are taken from equivalent locations (the vaginal fornix) and analyzed according to the same scoring criteria, there is no discernable difference in the diagnostic accuracy of the three smear-staining methods. The Pap-stained vaginal smears can be used as a wholly adequate alternative to Gram-stained smears for BV diagnosis. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Pap stain, bacterial vaginosis, diagnosis, vaginal smears, Amsel
in
Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS)
volume
115
issue
7
pages
809 - 813
publisher
John Wiley & Sons
external identifiers
  • wos:000247753100003
  • scopus:34447092159
ISSN
1600-0463
DOI
10.1111/j.1600-0463.2007.apm_607.x
language
English
LU publication?
yes
id
f807e3ff-1533-40d9-9336-f3bf28c3615f (old id 645977)
date added to LUP
2007-12-11 20:14:07
date last changed
2017-06-11 03:47:57
@article{f807e3ff-1533-40d9-9336-f3bf28c3615f,
  abstract     = {Papanicolaou-stained cervicovaginal smears (Pap smears) are used to screen for cervical cancer. Since there is a lack of consensus in published reports respecting the efficacy of Pap-stained smears in BV diagnostics, there is a need to validate their use for diagnosis of BV. Slides from the international BV00 workshop were Pap stained and independently analyzed by four investigators under a phase-contrast microscope. All workshop slides-whether Pap-stained, Gram-stained or rehydrated air-dried smears-were scored according to the same Nugent classification. The diagnostic accuracy of Pap smears for diagnosis of BV had a sensitivity of 0.85 and a specificity of 0.92, with a positive and negative predictive value of 0.84 and 0.93, respectively. The interobserver weighted kappa index was 0.86 for Pap-stained smears compared to 0.81 for Gram-stained smears, and 0.70 for rehydrated air-dried smears using the mean Nugent score as the criterion standard. Provided that the samples are taken from equivalent locations (the vaginal fornix) and analyzed according to the same scoring criteria, there is no discernable difference in the diagnostic accuracy of the three smear-staining methods. The Pap-stained vaginal smears can be used as a wholly adequate alternative to Gram-stained smears for BV diagnosis.},
  author       = {Eriksson, K. and Forsum, U. and Bjornerem, A. and Platz-Christensen, Jens Jörgen and Larsson, P. G.},
  issn         = {1600-0463},
  keyword      = {Pap stain,bacterial vaginosis,diagnosis,vaginal smears,Amsel},
  language     = {eng},
  number       = {7},
  pages        = {809--813},
  publisher    = {John Wiley & Sons},
  series       = {Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS)},
  title        = {Validation of the use of Pap-stained vaginal smears for diagnosis of bacterial vaginosis},
  url          = {http://dx.doi.org/10.1111/j.1600-0463.2007.apm_607.x},
  volume       = {115},
  year         = {2007},
}