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Expression pattern analysis of transcribed HERV sequences is complicated by ex vivo recombination

Flockerzi, Aline; Maydt, Jochen; Frank, Oliver; Ruggieri, Alessia; Maldener, Esther; Seifarth, Wolfgang; Medstrand, Patrik LU ; Lengauer, Thomas; Meyerhans, Andreas and Leib-Moesch, Christine, et al. (2007) In Retrovirology 4.
Abstract
Background: The human genome comprises numerous human endogenous retroviruses ( HERVs) that formed millions of years ago in ancestral species. A number of loci of the HERV-K(HML-2) family are evolutionarily much younger. A recent study suggested an infectious HERV-K(HML-2) variant in humans and other primates. Isolating such a varian t from human individuals would be a significant finding for human biology. Results: When investigating expression patterns of specific HML-2 proviruses we encountered HERV-K(HML-2) cDNA sequences without proviral homologues in the human genome, named HERV-KX, that could very well support recently suggested infectious HML-2 variants. However, detailed sequence analysis, using the software RECCO, suggested that... (More)
Background: The human genome comprises numerous human endogenous retroviruses ( HERVs) that formed millions of years ago in ancestral species. A number of loci of the HERV-K(HML-2) family are evolutionarily much younger. A recent study suggested an infectious HERV-K(HML-2) variant in humans and other primates. Isolating such a varian t from human individuals would be a significant finding for human biology. Results: When investigating expression patterns of specific HML-2 proviruses we encountered HERV-K(HML-2) cDNA sequences without proviral homologues in the human genome, named HERV-KX, that could very well support recently suggested infectious HML-2 variants. However, detailed sequence analysis, using the software RECCO, suggested that HERV-KX sequences were produced by recombination, possibly arising ex vivo, between transcripts from different HML-2 proviral loci. Conclusion: As RT-PCR probably will be instrumental for isolating an infectious HERV-K(HML2) variant, generation of "new" HERV-K(HML-2) sequences by ex vivo recombination seems inevitable. Further complicated by an unknown amount of allelic sequence variation in HERV-K(HML-2) proviruses, newly identified HERV-K(HML-2) variants should be interpreted very cautiously. (Less)
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Retrovirology
volume
4
publisher
BioMed Central
external identifiers
  • wos:000247633300001
  • scopus:34447301254
ISSN
1742-4690
DOI
10.1186/1742-4690-4-39
language
English
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yes
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9d401ba5-7ed1-4301-aa49-84fbe17e0c04 (old id 646162)
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2007-12-11 14:27:55
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2017-07-23 04:45:23
@article{9d401ba5-7ed1-4301-aa49-84fbe17e0c04,
  abstract     = {Background: The human genome comprises numerous human endogenous retroviruses ( HERVs) that formed millions of years ago in ancestral species. A number of loci of the HERV-K(HML-2) family are evolutionarily much younger. A recent study suggested an infectious HERV-K(HML-2) variant in humans and other primates. Isolating such a varian t from human individuals would be a significant finding for human biology. Results: When investigating expression patterns of specific HML-2 proviruses we encountered HERV-K(HML-2) cDNA sequences without proviral homologues in the human genome, named HERV-KX, that could very well support recently suggested infectious HML-2 variants. However, detailed sequence analysis, using the software RECCO, suggested that HERV-KX sequences were produced by recombination, possibly arising ex vivo, between transcripts from different HML-2 proviral loci. Conclusion: As RT-PCR probably will be instrumental for isolating an infectious HERV-K(HML2) variant, generation of "new" HERV-K(HML-2) sequences by ex vivo recombination seems inevitable. Further complicated by an unknown amount of allelic sequence variation in HERV-K(HML-2) proviruses, newly identified HERV-K(HML-2) variants should be interpreted very cautiously.},
  author       = {Flockerzi, Aline and Maydt, Jochen and Frank, Oliver and Ruggieri, Alessia and Maldener, Esther and Seifarth, Wolfgang and Medstrand, Patrik and Lengauer, Thomas and Meyerhans, Andreas and Leib-Moesch, Christine and Meese, Eckart and Mayer, Jens},
  issn         = {1742-4690},
  language     = {eng},
  publisher    = {BioMed Central},
  series       = {Retrovirology},
  title        = {Expression pattern analysis of transcribed HERV sequences is complicated by ex vivo recombination},
  url          = {http://dx.doi.org/10.1186/1742-4690-4-39},
  volume       = {4},
  year         = {2007},
}