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Proteomic variation is as large within as between strawberry varieties

Alm, Rikard LU ; Ekefjard, Andreas; Krogh, Morten LU ; Häkkinen, Jari LU and Emanuelsson, Cecilia LU (2007) In Journal of Proteome Research 6(8). p.3011-3020
Abstract
in search for a strawberry (Fragaria ananassa) with low allergen content, we determined the proteomic variation within and between different varieties. Proteomics data were generated by DIGE and proteins identified with MALDI-MS/MS. The amount of the strawberry allergen Fra a 1 varied between different strawberry varieties (CV = 39%). The variation was at the same level, or even slightly larger, due to different growth conditions (CV = 43%). For 153 other proteins, the biological variation was more affected by different growth conditions than by different varieties (mean CV = 52% and 43%, respectively) due to variation in a subset of proteins. Thus, the allergen variation due to growth conditions must be taken into consideration in... (More)
in search for a strawberry (Fragaria ananassa) with low allergen content, we determined the proteomic variation within and between different varieties. Proteomics data were generated by DIGE and proteins identified with MALDI-MS/MS. The amount of the strawberry allergen Fra a 1 varied between different strawberry varieties (CV = 39%). The variation was at the same level, or even slightly larger, due to different growth conditions (CV = 43%). For 153 other proteins, the biological variation was more affected by different growth conditions than by different varieties (mean CV = 52% and 43%, respectively) due to variation in a subset of proteins. Thus, the allergen variation due to growth conditions must be taken into consideration in attempts to obtain a low-allergen strawberry. However, the allergen content was always lower in colorless (white) strawberry varieties than in the red ones. Moreover, of the spots whose expression correlated with the allergen and the color (32 and 68, respectively), only 3 were the same. This implies that these two phenotypic traits are not inseparable, and it may be possible to breed a red strawberry with low amount of allergen. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
DIGE, growth conditions, allergen, phenotypic plasticity, variety, proteomics, biological variation, external validity, intraspecific
in
Journal of Proteome Research
volume
6
issue
8
pages
3011 - 3020
publisher
The American Chemical Society
external identifiers
  • wos:000248683200009
  • scopus:34548162709
ISSN
1535-3893
DOI
10.1021/pr0700450
language
English
LU publication?
yes
id
20dd632b-2564-40bd-9231-65dcadd2217e (old id 646352)
date added to LUP
2007-12-04 18:07:12
date last changed
2017-09-17 05:34:47
@article{20dd632b-2564-40bd-9231-65dcadd2217e,
  abstract     = {in search for a strawberry (Fragaria ananassa) with low allergen content, we determined the proteomic variation within and between different varieties. Proteomics data were generated by DIGE and proteins identified with MALDI-MS/MS. The amount of the strawberry allergen Fra a 1 varied between different strawberry varieties (CV = 39%). The variation was at the same level, or even slightly larger, due to different growth conditions (CV = 43%). For 153 other proteins, the biological variation was more affected by different growth conditions than by different varieties (mean CV = 52% and 43%, respectively) due to variation in a subset of proteins. Thus, the allergen variation due to growth conditions must be taken into consideration in attempts to obtain a low-allergen strawberry. However, the allergen content was always lower in colorless (white) strawberry varieties than in the red ones. Moreover, of the spots whose expression correlated with the allergen and the color (32 and 68, respectively), only 3 were the same. This implies that these two phenotypic traits are not inseparable, and it may be possible to breed a red strawberry with low amount of allergen.},
  author       = {Alm, Rikard and Ekefjard, Andreas and Krogh, Morten and Häkkinen, Jari and Emanuelsson, Cecilia},
  issn         = {1535-3893},
  keyword      = {DIGE,growth conditions,allergen,phenotypic plasticity,variety,proteomics,biological variation,external validity,intraspecific},
  language     = {eng},
  number       = {8},
  pages        = {3011--3020},
  publisher    = {The American Chemical Society},
  series       = {Journal of Proteome Research},
  title        = {Proteomic variation is as large within as between strawberry varieties},
  url          = {http://dx.doi.org/10.1021/pr0700450},
  volume       = {6},
  year         = {2007},
}