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Effect of headgroup on DNA - Cationic surfactant interactions

Dasgupta, Antara; Das, Prasanta Kumar; Dias, Rita LU ; Miguel, Maria LU and Lindman, Björn LU (2007) In The Journal of Physical Chemistry Part B 111(29). p.8502-8508
Abstract
The interaction behavior of DNA with different types of hydroxylated cationic surfactants has been studied. Attention was directed to how the introduction of hydroxyl substituents at the headgroup of the cationic surfactants affects the compaction of DNA. The DNA-cationic surfactant interaction was investigated at different charge ratios by several methods like UV melting, ethidium bromide exclusion, and gel electrophoresis. Studies show that there is a discrete transition in the DNA chain from extended coils (free chain) to a compact form and that this transition does not depend substantially on the architecture of the headgroup. However, the accessibility of DNA to ethidium bromide is preserved to a significantly larger extent for the... (More)
The interaction behavior of DNA with different types of hydroxylated cationic surfactants has been studied. Attention was directed to how the introduction of hydroxyl substituents at the headgroup of the cationic surfactants affects the compaction of DNA. The DNA-cationic surfactant interaction was investigated at different charge ratios by several methods like UV melting, ethidium bromide exclusion, and gel electrophoresis. Studies show that there is a discrete transition in the DNA chain from extended coils (free chain) to a compact form and that this transition does not depend substantially on the architecture of the headgroup. However, the accessibility of DNA to ethidium bromide is preserved to a significantly larger extent for the more hydrophilic surfactants. This was discussed in terms of surfactant packing. Observations are interpreted to reflect that the surfactants with more substituents have a larger headgroup and therefore form smaller micellar aggregates; these higher curvature aggregates lead to a less efficient, "patch-like" coverage of DNA. The more hydrophilic surfactants also presented a significantly lower cytotoxicity, which is important for biotechnological applications. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
The Journal of Physical Chemistry Part B
volume
111
issue
29
pages
8502 - 8508
publisher
The American Chemical Society
external identifiers
  • wos:000248121500023
  • scopus:34547674854
ISSN
1520-5207
DOI
10.1021/jp068571m
language
English
LU publication?
yes
id
30bfb6e9-be66-4d5e-8701-53fca683d95e (old id 646376)
date added to LUP
2007-12-06 14:06:41
date last changed
2017-10-22 04:40:20
@article{30bfb6e9-be66-4d5e-8701-53fca683d95e,
  abstract     = {The interaction behavior of DNA with different types of hydroxylated cationic surfactants has been studied. Attention was directed to how the introduction of hydroxyl substituents at the headgroup of the cationic surfactants affects the compaction of DNA. The DNA-cationic surfactant interaction was investigated at different charge ratios by several methods like UV melting, ethidium bromide exclusion, and gel electrophoresis. Studies show that there is a discrete transition in the DNA chain from extended coils (free chain) to a compact form and that this transition does not depend substantially on the architecture of the headgroup. However, the accessibility of DNA to ethidium bromide is preserved to a significantly larger extent for the more hydrophilic surfactants. This was discussed in terms of surfactant packing. Observations are interpreted to reflect that the surfactants with more substituents have a larger headgroup and therefore form smaller micellar aggregates; these higher curvature aggregates lead to a less efficient, "patch-like" coverage of DNA. The more hydrophilic surfactants also presented a significantly lower cytotoxicity, which is important for biotechnological applications.},
  author       = {Dasgupta, Antara and Das, Prasanta Kumar and Dias, Rita and Miguel, Maria and Lindman, Björn},
  issn         = {1520-5207},
  language     = {eng},
  number       = {29},
  pages        = {8502--8508},
  publisher    = {The American Chemical Society},
  series       = {The Journal of Physical Chemistry Part B},
  title        = {Effect of headgroup on DNA - Cationic surfactant interactions},
  url          = {http://dx.doi.org/10.1021/jp068571m},
  volume       = {111},
  year         = {2007},
}