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Platination of the siRNA sense-strand modulates both efficacy and selectivity in vitro

Hägerlöf, Margareta LU ; Hedman, Hanna LU and Elmroth, Sofi LU (2007) In Biochemical and Biophysical Research Communications 361(1). p.14-19
Abstract
The use of short interfering RNAs (siRNA) for selective suppression of protein production has rapidly become a commonly used technique for transient modulation of protein levels. In the present paper, we investigate whether introduction of platinated bases in the sense strand can be used to modulate the efficacy of siRNAs. Four different siRNAs were studied, all targeting the initial AU-rich 3' UTR of Wnt-5a mRNA. The siRNAs were characterized with respect to melting properties and translational inhibitory effect in vitro using luciferase as a reporter gene. The translation inhibition studies reveal that all platinated siRNA remain efficient. For an siRNA with partial complementarity to the lucifierase gene, platination was shown to reduce... (More)
The use of short interfering RNAs (siRNA) for selective suppression of protein production has rapidly become a commonly used technique for transient modulation of protein levels. In the present paper, we investigate whether introduction of platinated bases in the sense strand can be used to modulate the efficacy of siRNAs. Four different siRNAs were studied, all targeting the initial AU-rich 3' UTR of Wnt-5a mRNA. The siRNAs were characterized with respect to melting properties and translational inhibitory effect in vitro using luciferase as a reporter gene. The translation inhibition studies reveal that all platinated siRNA remain efficient. For an siRNA with partial complementarity to the lucifierase gene, platination was shown to reduce the off-target effects. All siRNAs were found to be active in cellular in vitro translation systems, reaching suppression levels well above 80% for the majority of siRNAs investigated. (c) 2007 Elsevier Inc. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
down, RNA, 3' UTR, wnt-5a, platinum, siRNA, off-target minimization, regulation
in
Biochemical and Biophysical Research Communications
volume
361
issue
1
pages
14 - 19
publisher
Elsevier
external identifiers
  • wos:000248659000003
  • scopus:34548665529
ISSN
1090-2104
DOI
10.1016/j.bbrc.2007.06.131
language
English
LU publication?
yes
id
5f4c93e1-acac-4ea2-8f4c-7a67e8510d95 (old id 647575)
date added to LUP
2007-12-10 16:18:08
date last changed
2017-01-01 07:11:33
@article{5f4c93e1-acac-4ea2-8f4c-7a67e8510d95,
  abstract     = {The use of short interfering RNAs (siRNA) for selective suppression of protein production has rapidly become a commonly used technique for transient modulation of protein levels. In the present paper, we investigate whether introduction of platinated bases in the sense strand can be used to modulate the efficacy of siRNAs. Four different siRNAs were studied, all targeting the initial AU-rich 3' UTR of Wnt-5a mRNA. The siRNAs were characterized with respect to melting properties and translational inhibitory effect in vitro using luciferase as a reporter gene. The translation inhibition studies reveal that all platinated siRNA remain efficient. For an siRNA with partial complementarity to the lucifierase gene, platination was shown to reduce the off-target effects. All siRNAs were found to be active in cellular in vitro translation systems, reaching suppression levels well above 80% for the majority of siRNAs investigated. (c) 2007 Elsevier Inc. All rights reserved.},
  author       = {Hägerlöf, Margareta and Hedman, Hanna and Elmroth, Sofi},
  issn         = {1090-2104},
  keyword      = {down,RNA,3' UTR,wnt-5a,platinum,siRNA,off-target minimization,regulation},
  language     = {eng},
  number       = {1},
  pages        = {14--19},
  publisher    = {Elsevier},
  series       = {Biochemical and Biophysical Research Communications},
  title        = {Platination of the siRNA sense-strand modulates both efficacy and selectivity in vitro},
  url          = {http://dx.doi.org/10.1016/j.bbrc.2007.06.131},
  volume       = {361},
  year         = {2007},
}