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Clinical-scale generation of strongly CD83-expressing dendritic cells using extracorporeal photopheresis

Bredberg, Anders LU ; Jonsson, Svante LU ; Lindblom, Anders and Björk, Peter LU (2007) In Photodermatology, Photoimmunology & Photomedicine 23(4). p.113-119
Abstract
Background: Many strategies are currently being pursued in order to generate mature dendritic cells (DC) to be used for immunotherapy. A potent anti-tumour influence by extracorporeal photopheresis has been documented for cutaneous T-cell lymphoma, and a major mechanism of action has been suggested to be generation of DC presenting tumour antigens. Purpose: To determine the potential of a simple clinical photopheresis protocol for large-scale development of mature DC. Methods: A standard monocyte-enriched leukapheresis preparation of 10(9)-10(10) cells was derived during each of five consecutive treatment sessions of a patient with cutaneous T-cell lymphoma. The cells were incubated overnight in autologous plasma with no addition of growth... (More)
Background: Many strategies are currently being pursued in order to generate mature dendritic cells (DC) to be used for immunotherapy. A potent anti-tumour influence by extracorporeal photopheresis has been documented for cutaneous T-cell lymphoma, and a major mechanism of action has been suggested to be generation of DC presenting tumour antigens. Purpose: To determine the potential of a simple clinical photopheresis protocol for large-scale development of mature DC. Methods: A standard monocyte-enriched leukapheresis preparation of 10(9)-10(10) cells was derived during each of five consecutive treatment sessions of a patient with cutaneous T-cell lymphoma. The cells were incubated overnight in autologous plasma with no addition of growth medium. Cell surface lymphocyte, monocyte and DC markers were determined using multi-colour flow cytometry. Results: We find signs of activation of the CD14+ monocytes, as well as the appearance of a minor population of mature DC negative for CD14 but with strong CD83 expression. Conclusions: With a procedure appropriate for routine clinical use, a total number of 10(6)-10(7) DC ready for patient reinfusion can be prepared within 24 h. Our findings indicate the need to further explore the capacity of photopheresis to stimulate cancer patients' anti-tumour defence reaction. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
cancer immunotherapy, extracorporeal, dendritic cells, CD83, photopheresis, monocytes, clinical scale
in
Photodermatology, Photoimmunology & Photomedicine
volume
23
issue
4
pages
113 - 119
publisher
Wiley-Blackwell
external identifiers
  • wos:000247583000002
  • scopus:34447121562
ISSN
1600-0781
DOI
10.1111/j.1600-0781.2007.00285.x
language
English
LU publication?
yes
id
3ead789e-d28f-47d0-a60a-ce65ceed7f73 (old id 647654)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17598863&dopt=Abstract
date added to LUP
2007-12-11 15:51:35
date last changed
2017-01-01 04:32:50
@article{3ead789e-d28f-47d0-a60a-ce65ceed7f73,
  abstract     = {Background: Many strategies are currently being pursued in order to generate mature dendritic cells (DC) to be used for immunotherapy. A potent anti-tumour influence by extracorporeal photopheresis has been documented for cutaneous T-cell lymphoma, and a major mechanism of action has been suggested to be generation of DC presenting tumour antigens. Purpose: To determine the potential of a simple clinical photopheresis protocol for large-scale development of mature DC. Methods: A standard monocyte-enriched leukapheresis preparation of 10(9)-10(10) cells was derived during each of five consecutive treatment sessions of a patient with cutaneous T-cell lymphoma. The cells were incubated overnight in autologous plasma with no addition of growth medium. Cell surface lymphocyte, monocyte and DC markers were determined using multi-colour flow cytometry. Results: We find signs of activation of the CD14+ monocytes, as well as the appearance of a minor population of mature DC negative for CD14 but with strong CD83 expression. Conclusions: With a procedure appropriate for routine clinical use, a total number of 10(6)-10(7) DC ready for patient reinfusion can be prepared within 24 h. Our findings indicate the need to further explore the capacity of photopheresis to stimulate cancer patients' anti-tumour defence reaction.},
  author       = {Bredberg, Anders and Jonsson, Svante and Lindblom, Anders and Björk, Peter},
  issn         = {1600-0781},
  keyword      = {cancer immunotherapy,extracorporeal,dendritic cells,CD83,photopheresis,monocytes,clinical scale},
  language     = {eng},
  number       = {4},
  pages        = {113--119},
  publisher    = {Wiley-Blackwell},
  series       = {Photodermatology, Photoimmunology & Photomedicine},
  title        = {Clinical-scale generation of strongly CD83-expressing dendritic cells using extracorporeal photopheresis},
  url          = {http://dx.doi.org/10.1111/j.1600-0781.2007.00285.x},
  volume       = {23},
  year         = {2007},
}