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Macroporous gels prepared at subzero temperatures as novel materials for chromatography of particulate-containing fluids and cell culture applications

Plieva, Fatima LU ; Galaev, Igor LU and Mattiasson, Bo LU (2007) In Journal of Separation Science 30(11). p.1657-1671
Abstract
Macroporous gels (MGs) with a broad variety of morphologies are prepared using the cryotropic gelation technique, i.e. gelation at subzero temperatures. These highly elastic hydrophilic materials can be produced from practically any gel-forming system with a broad range of porosity extending from elastic and porous gels with pore sizes up to 1.0 mu m to elastic and sponge-like gels with pore sizes up to 100 mu m. The versatility of the cryogelation technique is demonstrated by use of different chemical reactions (hydrogen bond formation, chemical cross-linking of polymers, free radical polymerization) mainly in an aqueous medium. Appropriate control over solvent crystallization (formation of solvent crystals) and rate of chemical reaction... (More)
Macroporous gels (MGs) with a broad variety of morphologies are prepared using the cryotropic gelation technique, i.e. gelation at subzero temperatures. These highly elastic hydrophilic materials can be produced from practically any gel-forming system with a broad range of porosity extending from elastic and porous gels with pore sizes up to 1.0 mu m to elastic and sponge-like gels with pore sizes up to 100 mu m. The versatility of the cryogelation technique is demonstrated by use of different chemical reactions (hydrogen bond formation, chemical cross-linking of polymers, free radical polymerization) mainly in an aqueous medium. Appropriate control over solvent crystallization (formation of solvent crystals) and rate of chemical reaction during the cryogelation allows the reproducible preparation of cryogels with tailored properties. Different approaches, such as chemical modification of reactive groups, grafting of the pore surface with an appropriate polymer, or direct copolymerization with functional monomers are used for control of the surface chemistry of MGs. Typically, MGs with pore sizes up to 1.0 mu m are produced in the shape of beads and MGs with pore size up to 100 mu m are prepared as monoliths, discs, and sheets. The difference in porous structure of MGs defines the main applications of these porous materials. Elastic beaded MGs are mostly used as carriers for. cell and enzyme immobilization or for capture of low-molecular weight targets from particulate-containing fluids in expanded-bed mode. However, the elastic and sponge-like MG monoliths with interconnected pores measuring hundreds of gm have been successfully used as monolithic columns for chromatography of particulate-containing fluids (crude cell homogenates, viruses, whole cells, wastewater effluents) and as three-dimensional scaffolds for mammalian cell culture applications. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
cryogel, 3-D scaffold, cell chromatography, macroporous gel, monolith
in
Journal of Separation Science
volume
30
issue
11
pages
1657 - 1671
publisher
John Wiley & Sons
external identifiers
  • wos:000248361100006
  • scopus:34547598638
ISSN
1615-9314
DOI
10.1002/jssc.200700127
language
English
LU publication?
yes
id
d791744d-242a-476e-8067-b3a65b615ae4 (old id 647675)
date added to LUP
2007-12-14 09:10:33
date last changed
2017-10-22 03:54:45
@article{d791744d-242a-476e-8067-b3a65b615ae4,
  abstract     = {Macroporous gels (MGs) with a broad variety of morphologies are prepared using the cryotropic gelation technique, i.e. gelation at subzero temperatures. These highly elastic hydrophilic materials can be produced from practically any gel-forming system with a broad range of porosity extending from elastic and porous gels with pore sizes up to 1.0 mu m to elastic and sponge-like gels with pore sizes up to 100 mu m. The versatility of the cryogelation technique is demonstrated by use of different chemical reactions (hydrogen bond formation, chemical cross-linking of polymers, free radical polymerization) mainly in an aqueous medium. Appropriate control over solvent crystallization (formation of solvent crystals) and rate of chemical reaction during the cryogelation allows the reproducible preparation of cryogels with tailored properties. Different approaches, such as chemical modification of reactive groups, grafting of the pore surface with an appropriate polymer, or direct copolymerization with functional monomers are used for control of the surface chemistry of MGs. Typically, MGs with pore sizes up to 1.0 mu m are produced in the shape of beads and MGs with pore size up to 100 mu m are prepared as monoliths, discs, and sheets. The difference in porous structure of MGs defines the main applications of these porous materials. Elastic beaded MGs are mostly used as carriers for. cell and enzyme immobilization or for capture of low-molecular weight targets from particulate-containing fluids in expanded-bed mode. However, the elastic and sponge-like MG monoliths with interconnected pores measuring hundreds of gm have been successfully used as monolithic columns for chromatography of particulate-containing fluids (crude cell homogenates, viruses, whole cells, wastewater effluents) and as three-dimensional scaffolds for mammalian cell culture applications.},
  author       = {Plieva, Fatima and Galaev, Igor and Mattiasson, Bo},
  issn         = {1615-9314},
  keyword      = {cryogel,3-D scaffold,cell chromatography,macroporous gel,monolith},
  language     = {eng},
  number       = {11},
  pages        = {1657--1671},
  publisher    = {John Wiley & Sons},
  series       = {Journal of Separation Science},
  title        = {Macroporous gels prepared at subzero temperatures as novel materials for chromatography of particulate-containing fluids and cell culture applications},
  url          = {http://dx.doi.org/10.1002/jssc.200700127},
  volume       = {30},
  year         = {2007},
}