Correlating two methods of quantifying fungal activity: Heat production by isothermal calorimetry and ergosterol amount by gas chromatography-tandem mass spectrometry
(2007) In Thermochimica Acta 458(1-2). p.77-83- Abstract
- Two methods of quantifying fungal activity have been compared and correlated: isothermal calorimetry for measuring heat production and gas chromatography-tandem mass spectrometry (GC-MS/MS) for measuring ergosterol, a proxy for biomass. The measurements were made on four different fungi: Penicillium roqueforti, Cladosporiurn cladosporioides, Neopetromyces muricatus and the dry rot fungus Serpula lacrymans. The results showed linear correlations between ergosterol production and total heat production for these four fungal species during the initial fast growing stage. At the later stages heat was produced but ergosterol amount was constant. The heat produced per ergosterol amount varied from species to species and between different... (More)
- Two methods of quantifying fungal activity have been compared and correlated: isothermal calorimetry for measuring heat production and gas chromatography-tandem mass spectrometry (GC-MS/MS) for measuring ergosterol, a proxy for biomass. The measurements were made on four different fungi: Penicillium roqueforti, Cladosporiurn cladosporioides, Neopetromyces muricatus and the dry rot fungus Serpula lacrymans. The results showed linear correlations between ergosterol production and total heat production for these four fungal species during the initial fast growing stage. At the later stages heat was produced but ergosterol amount was constant. The heat produced per ergosterol amount varied from species to species and between different temperatures. This might be due to the different metabolic efficiencies of different species or the same species at different temperatures. Isothermal calorimetry can be used in fungal studies on its own or in combination with other techniques for a more complete understanding of fungal physiology. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/648638
- author
- Li, Yujing LU ; Wadsö, Lars LU ; Larsson, Lennart LU and Bjurman, Jonny
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- gas chromatography-tandem mass spectrometry, isothermal calorimetry, ergosterol, GC-MS/MS, fungal activity, heat
- in
- Thermochimica Acta
- volume
- 458
- issue
- 1-2
- pages
- 77 - 83
- publisher
- Elsevier
- external identifiers
-
- wos:000247198800013
- scopus:34247857470
- ISSN
- 0040-6031
- DOI
- 10.1016/j.tca.2007.01.005
- language
- English
- LU publication?
- yes
- id
- 9998379e-59e6-4815-aaae-2236fc2a3ada (old id 648638)
- date added to LUP
- 2016-04-01 16:49:09
- date last changed
- 2022-03-22 21:24:41
@article{9998379e-59e6-4815-aaae-2236fc2a3ada, abstract = {{Two methods of quantifying fungal activity have been compared and correlated: isothermal calorimetry for measuring heat production and gas chromatography-tandem mass spectrometry (GC-MS/MS) for measuring ergosterol, a proxy for biomass. The measurements were made on four different fungi: Penicillium roqueforti, Cladosporiurn cladosporioides, Neopetromyces muricatus and the dry rot fungus Serpula lacrymans. The results showed linear correlations between ergosterol production and total heat production for these four fungal species during the initial fast growing stage. At the later stages heat was produced but ergosterol amount was constant. The heat produced per ergosterol amount varied from species to species and between different temperatures. This might be due to the different metabolic efficiencies of different species or the same species at different temperatures. Isothermal calorimetry can be used in fungal studies on its own or in combination with other techniques for a more complete understanding of fungal physiology.}}, author = {{Li, Yujing and Wadsö, Lars and Larsson, Lennart and Bjurman, Jonny}}, issn = {{0040-6031}}, keywords = {{gas chromatography-tandem mass spectrometry; isothermal calorimetry; ergosterol; GC-MS/MS; fungal activity; heat}}, language = {{eng}}, number = {{1-2}}, pages = {{77--83}}, publisher = {{Elsevier}}, series = {{Thermochimica Acta}}, title = {{Correlating two methods of quantifying fungal activity: Heat production by isothermal calorimetry and ergosterol amount by gas chromatography-tandem mass spectrometry}}, url = {{http://dx.doi.org/10.1016/j.tca.2007.01.005}}, doi = {{10.1016/j.tca.2007.01.005}}, volume = {{458}}, year = {{2007}}, }