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Correlating two methods of quantifying fungal activity: Heat production by isothermal calorimetry and ergosterol amount by gas chromatography-tandem mass spectrometry

Li, Yujing LU ; Wadsö, Lars LU ; Larsson, Lennart LU and Bjurman, Jonny (2007) In Thermochimica Acta 458(1-2). p.77-83
Abstract
Two methods of quantifying fungal activity have been compared and correlated: isothermal calorimetry for measuring heat production and gas chromatography-tandem mass spectrometry (GC-MS/MS) for measuring ergosterol, a proxy for biomass. The measurements were made on four different fungi: Penicillium roqueforti, Cladosporiurn cladosporioides, Neopetromyces muricatus and the dry rot fungus Serpula lacrymans. The results showed linear correlations between ergosterol production and total heat production for these four fungal species during the initial fast growing stage. At the later stages heat was produced but ergosterol amount was constant. The heat produced per ergosterol amount varied from species to species and between different... (More)
Two methods of quantifying fungal activity have been compared and correlated: isothermal calorimetry for measuring heat production and gas chromatography-tandem mass spectrometry (GC-MS/MS) for measuring ergosterol, a proxy for biomass. The measurements were made on four different fungi: Penicillium roqueforti, Cladosporiurn cladosporioides, Neopetromyces muricatus and the dry rot fungus Serpula lacrymans. The results showed linear correlations between ergosterol production and total heat production for these four fungal species during the initial fast growing stage. At the later stages heat was produced but ergosterol amount was constant. The heat produced per ergosterol amount varied from species to species and between different temperatures. This might be due to the different metabolic efficiencies of different species or the same species at different temperatures. Isothermal calorimetry can be used in fungal studies on its own or in combination with other techniques for a more complete understanding of fungal physiology. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
gas chromatography-tandem mass spectrometry, isothermal calorimetry, ergosterol, GC-MS/MS, fungal activity, heat
in
Thermochimica Acta
volume
458
issue
1-2
pages
77 - 83
publisher
Elsevier
external identifiers
  • wos:000247198800013
  • scopus:34247857470
ISSN
0040-6031
DOI
10.1016/j.tca.2007.01.005
language
English
LU publication?
yes
id
9998379e-59e6-4815-aaae-2236fc2a3ada (old id 648638)
date added to LUP
2007-12-20 10:58:58
date last changed
2017-02-26 04:17:19
@article{9998379e-59e6-4815-aaae-2236fc2a3ada,
  abstract     = {Two methods of quantifying fungal activity have been compared and correlated: isothermal calorimetry for measuring heat production and gas chromatography-tandem mass spectrometry (GC-MS/MS) for measuring ergosterol, a proxy for biomass. The measurements were made on four different fungi: Penicillium roqueforti, Cladosporiurn cladosporioides, Neopetromyces muricatus and the dry rot fungus Serpula lacrymans. The results showed linear correlations between ergosterol production and total heat production for these four fungal species during the initial fast growing stage. At the later stages heat was produced but ergosterol amount was constant. The heat produced per ergosterol amount varied from species to species and between different temperatures. This might be due to the different metabolic efficiencies of different species or the same species at different temperatures. Isothermal calorimetry can be used in fungal studies on its own or in combination with other techniques for a more complete understanding of fungal physiology.},
  author       = {Li, Yujing and Wadsö, Lars and Larsson, Lennart and Bjurman, Jonny},
  issn         = {0040-6031},
  keyword      = {gas chromatography-tandem mass spectrometry,isothermal calorimetry,ergosterol,GC-MS/MS,fungal activity,heat},
  language     = {eng},
  number       = {1-2},
  pages        = {77--83},
  publisher    = {Elsevier},
  series       = {Thermochimica Acta},
  title        = {Correlating two methods of quantifying fungal activity: Heat production by isothermal calorimetry and ergosterol amount by gas chromatography-tandem mass spectrometry},
  url          = {http://dx.doi.org/10.1016/j.tca.2007.01.005},
  volume       = {458},
  year         = {2007},
}