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The early postnatal pattern of vesicle formation in different regions of the porcine small intestine

Elbrond, V. S. and Weström, Björn LU (2007) In Livestock Science 108(1-3). p.142-145
Abstract
In the early postnatal period, the permeability of the piglet small intestine is high to compensate for the absence of trans-placental transfer of immunoglobulins during the fetal period. Vesicles, which mainly reflect the uptake of macromolecules and other colostral/ milk components, were studied in three different regions of the small intestine - proximal, mid and distal - in a total of twelve piglets on day 0 (unsuckled and colostrums-fed), 2 and 6 (all suckled). Tissues were sampled and prepared for light microscopy (paraffin and cryo) and trans-electron microscopy. Different methods were applied to visualize cytoplasmatic subcellular components such as fat (Oil red O) and carbohydrates (PAS). Appearance and morphology of the... (More)
In the early postnatal period, the permeability of the piglet small intestine is high to compensate for the absence of trans-placental transfer of immunoglobulins during the fetal period. Vesicles, which mainly reflect the uptake of macromolecules and other colostral/ milk components, were studied in three different regions of the small intestine - proximal, mid and distal - in a total of twelve piglets on day 0 (unsuckled and colostrums-fed), 2 and 6 (all suckled). Tissues were sampled and prepared for light microscopy (paraffin and cryo) and trans-electron microscopy. Different methods were applied to visualize cytoplasmatic subcellular components such as fat (Oil red O) and carbohydrates (PAS). Appearance and morphology of the epithelial vesicles were compared. In the proximal region several small supranuclear and a single large subnuclear electron dense, eosinophilic and PAS+ vesicle were present. They disappeared after 2 days (gut closure) and on day 6 adult-looking epithelial cells were present. In the distal region of day 0 pigs digestion vesicles/flocculent vesicles were observed in the cytoplasma. The vesicles appeared empty but with eosinophilic, PAS+ and electron dense precipitations. The size and variation of these vesicles increased with age. Fat absorption increased markedly from day 2 to day 6. The observations indicate that in general colostral absorption processes are initiated prenatally, persist after birth and in the distal region also after gut closure. Fat absorption increased after gut closure, and in the distal region the increase correlated with e.g. IgG absorption. (c) 2007 Elsevier B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
absorption, piglet, jejunum, fat, macromolecules
in
Livestock Science
volume
108
issue
1-3
pages
142 - 145
publisher
Elsevier
external identifiers
  • wos:000247123100033
  • scopus:34247548835
ISSN
1871-1413
DOI
10.1016/j.livsci.2007.01.028
language
English
LU publication?
yes
id
0b3e0637-858f-4e38-a2ac-2da91a1362bf (old id 648772)
date added to LUP
2007-12-07 14:48:37
date last changed
2017-01-01 06:47:11
@article{0b3e0637-858f-4e38-a2ac-2da91a1362bf,
  abstract     = {In the early postnatal period, the permeability of the piglet small intestine is high to compensate for the absence of trans-placental transfer of immunoglobulins during the fetal period. Vesicles, which mainly reflect the uptake of macromolecules and other colostral/ milk components, were studied in three different regions of the small intestine - proximal, mid and distal - in a total of twelve piglets on day 0 (unsuckled and colostrums-fed), 2 and 6 (all suckled). Tissues were sampled and prepared for light microscopy (paraffin and cryo) and trans-electron microscopy. Different methods were applied to visualize cytoplasmatic subcellular components such as fat (Oil red O) and carbohydrates (PAS). Appearance and morphology of the epithelial vesicles were compared. In the proximal region several small supranuclear and a single large subnuclear electron dense, eosinophilic and PAS+ vesicle were present. They disappeared after 2 days (gut closure) and on day 6 adult-looking epithelial cells were present. In the distal region of day 0 pigs digestion vesicles/flocculent vesicles were observed in the cytoplasma. The vesicles appeared empty but with eosinophilic, PAS+ and electron dense precipitations. The size and variation of these vesicles increased with age. Fat absorption increased markedly from day 2 to day 6. The observations indicate that in general colostral absorption processes are initiated prenatally, persist after birth and in the distal region also after gut closure. Fat absorption increased after gut closure, and in the distal region the increase correlated with e.g. IgG absorption. (c) 2007 Elsevier B.V. All rights reserved.},
  author       = {Elbrond, V. S. and Weström, Björn},
  issn         = {1871-1413},
  keyword      = {absorption,piglet,jejunum,fat,macromolecules},
  language     = {eng},
  number       = {1-3},
  pages        = {142--145},
  publisher    = {Elsevier},
  series       = {Livestock Science},
  title        = {The early postnatal pattern of vesicle formation in different regions of the porcine small intestine},
  url          = {http://dx.doi.org/10.1016/j.livsci.2007.01.028},
  volume       = {108},
  year         = {2007},
}