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Combined serum IgG response to Helicobacter pylori VacA and CagA predicts gastric cancer

Janulaityte-Gunther, Daiva; Kupcinskas, Limas; Pavilonis, Alvydas; Valuckas, Konstantinas; Wadström, Torkel LU and Andersen, Leif Percival (2007) In Pathogens and Disease 50(2). p.220-225
Abstract
Helicobacter pylori is a major factor for the development of gastric cancer. The aim of this study was to define serum antibody patterns associated with H. pylori infection in patients with gastric cancer using a Western blot technique. Serum samples collected from 115 patients with gastric cancer and 110 age- and gender-matched patients without gastrointestinal diseases were tested for IgG antibodies to H. pylori antigens (outer membrane proteins and whole cell preparations). No significant differences were found between patients with and without gastric cancer using outer membrane proteins (82% and 73%, P > 0.05) or whole cell antigens (84% and 76%, P > 0.05), respectively. The significant differences between patients with and... (More)
Helicobacter pylori is a major factor for the development of gastric cancer. The aim of this study was to define serum antibody patterns associated with H. pylori infection in patients with gastric cancer using a Western blot technique. Serum samples collected from 115 patients with gastric cancer and 110 age- and gender-matched patients without gastrointestinal diseases were tested for IgG antibodies to H. pylori antigens (outer membrane proteins and whole cell preparations). No significant differences were found between patients with and without gastric cancer using outer membrane proteins (82% and 73%, P > 0.05) or whole cell antigens (84% and 76%, P > 0.05), respectively. The significant differences between patients with and without gastric cancer were associated with bands of 94 kDa (54% and 20%, P < 0.001) and 30 kDa (65% and 44%, P < 0.01). A combination of antibodies to 85 kDa (VacA) and 120 kDa (CagA) was significantly (P < 0.01) more frequent in gastric cancer patients than in patients without gastric cancer. The detection of antibodies to 94- and 30-kDa bands, in association with the determination of serum antibodies to CagA(+)/VacA(+), may have a prospective value in assessment of the risk of developing of gastric cancer. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
CagA, Helicobacter pylori, gastric cancer, VacA, Western blot, immunoblot
in
Pathogens and Disease
volume
50
issue
2
pages
220 - 225
publisher
Wiley-Blackwell
external identifiers
  • wos:000247226000010
  • scopus:34250319670
ISSN
2049-632X
DOI
10.1111/j.1574-695X.2007.00268.x
language
English
LU publication?
yes
id
c35f8fc5-044c-459d-8496-79ba9a43ee3b (old id 648912)
date added to LUP
2007-12-13 10:23:20
date last changed
2017-05-21 03:32:07
@article{c35f8fc5-044c-459d-8496-79ba9a43ee3b,
  abstract     = {Helicobacter pylori is a major factor for the development of gastric cancer. The aim of this study was to define serum antibody patterns associated with H. pylori infection in patients with gastric cancer using a Western blot technique. Serum samples collected from 115 patients with gastric cancer and 110 age- and gender-matched patients without gastrointestinal diseases were tested for IgG antibodies to H. pylori antigens (outer membrane proteins and whole cell preparations). No significant differences were found between patients with and without gastric cancer using outer membrane proteins (82% and 73%, P &gt; 0.05) or whole cell antigens (84% and 76%, P &gt; 0.05), respectively. The significant differences between patients with and without gastric cancer were associated with bands of 94 kDa (54% and 20%, P &lt; 0.001) and 30 kDa (65% and 44%, P &lt; 0.01). A combination of antibodies to 85 kDa (VacA) and 120 kDa (CagA) was significantly (P &lt; 0.01) more frequent in gastric cancer patients than in patients without gastric cancer. The detection of antibodies to 94- and 30-kDa bands, in association with the determination of serum antibodies to CagA(+)/VacA(+), may have a prospective value in assessment of the risk of developing of gastric cancer.},
  author       = {Janulaityte-Gunther, Daiva and Kupcinskas, Limas and Pavilonis, Alvydas and Valuckas, Konstantinas and Wadström, Torkel and Andersen, Leif Percival},
  issn         = {2049-632X},
  keyword      = {CagA,Helicobacter pylori,gastric cancer,VacA,Western blot,immunoblot},
  language     = {eng},
  number       = {2},
  pages        = {220--225},
  publisher    = {Wiley-Blackwell},
  series       = {Pathogens and Disease},
  title        = {Combined serum IgG response to Helicobacter pylori VacA and CagA predicts gastric cancer},
  url          = {http://dx.doi.org/10.1111/j.1574-695X.2007.00268.x},
  volume       = {50},
  year         = {2007},
}