Different assessments of immunohistochemically stained Ki-67 and hTERT in glioblastoma multiforme yield variable results: a study with reference to survival prognosis.
Persson, Annette LU and Englund, Elisabet LU
(2008)
In Clinical Neuropathology
27(4).
p.224-233
- Abstract
- OBJECTIVE: To investigate a marker of tumor proliferation, Ki-67, and telomerase expression in glioblastoma multiforme and to compare the results of different mainly quantitative assessments, in relation to age and survival rates. METHODS: Immunohistochemical stainings of Ki-67 and hTERT were evaluated in 39 formaldehyde-fixed, paraffin-embedded surgical samples of glioblastoma multiforme diagnosed during 2004, comprising all specimens with sufficient amount of vital tissue sent to the Department of Pathology during this year. Ki-67 counting and hTERT evaluation was assessed on whole tumor sections and on selected areas within each section. Age and length of survival were analyzed in relation to these parameters. RESULTS: We found that... (More)
- OBJECTIVE: To investigate a marker of tumor proliferation, Ki-67, and telomerase expression in glioblastoma multiforme and to compare the results of different mainly quantitative assessments, in relation to age and survival rates. METHODS: Immunohistochemical stainings of Ki-67 and hTERT were evaluated in 39 formaldehyde-fixed, paraffin-embedded surgical samples of glioblastoma multiforme diagnosed during 2004, comprising all specimens with sufficient amount of vital tissue sent to the Department of Pathology during this year. Ki-67 counting and hTERT evaluation was assessed on whole tumor sections and on selected areas within each section. Age and length of survival were analyzed in relation to these parameters. RESULTS: We found that different methods of evaluating the stained sections yielded different results regarding Ki-67, but less marked differences for hTERT. With Ki-67 counting on whole sections (non-selected areas), we found a statistically significant correlation with length of survival. There was no corresponding information in the hTERT assessment. We could also confirm a significant inverse correlation between age and length of survival, as previously published. CONCLUSION: Our data demonstrate that different methods of Ki-67 evaluation may give markedly dissimilar results. The significant correlation found between survival and one but not with two other methods of Ki-67 assessment, implicate the value of standardized quantification methods. Our data indicate a possible prognostic use of immunohistochemical Ki-67 proliferation index in glioblastoma multiforme. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1223683
- author
- Persson, Annette
LU
and Englund, Elisabet
LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Ki-67 Antigen: biosynthesis, Glioblastoma: pathology, Glioblastoma: mortality, Glioblastoma: metabolism, Brain Neoplasms: pathology, Brain Neoplasms: metabolism, Brain Neoplasms: mortality, Telomerase: biosynthesis, Tumor Markers, Biological: analysis
- in
- Clinical Neuropathology
- volume
- 27
- issue
- 4
- pages
- 224 - 233
- publisher
- Dustri-Verlag
- external identifiers
-
- wos:000257663800002
- pmid:18666438
- scopus:47349129485
- ISSN
- 0722-5091
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Pathology, (Lund) (013030000)
- id
- 648c8bc2-67d8-432a-8f8a-cb3aae5da41b (old id 1223683)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/18666438?dopt=Abstract
- date added to LUP
- 2016-04-04 09:23:16
- date last changed
- 2022-01-29 17:38:31
@article{648c8bc2-67d8-432a-8f8a-cb3aae5da41b,
abstract = {{OBJECTIVE: To investigate a marker of tumor proliferation, Ki-67, and telomerase expression in glioblastoma multiforme and to compare the results of different mainly quantitative assessments, in relation to age and survival rates. METHODS: Immunohistochemical stainings of Ki-67 and hTERT were evaluated in 39 formaldehyde-fixed, paraffin-embedded surgical samples of glioblastoma multiforme diagnosed during 2004, comprising all specimens with sufficient amount of vital tissue sent to the Department of Pathology during this year. Ki-67 counting and hTERT evaluation was assessed on whole tumor sections and on selected areas within each section. Age and length of survival were analyzed in relation to these parameters. RESULTS: We found that different methods of evaluating the stained sections yielded different results regarding Ki-67, but less marked differences for hTERT. With Ki-67 counting on whole sections (non-selected areas), we found a statistically significant correlation with length of survival. There was no corresponding information in the hTERT assessment. We could also confirm a significant inverse correlation between age and length of survival, as previously published. CONCLUSION: Our data demonstrate that different methods of Ki-67 evaluation may give markedly dissimilar results. The significant correlation found between survival and one but not with two other methods of Ki-67 assessment, implicate the value of standardized quantification methods. Our data indicate a possible prognostic use of immunohistochemical Ki-67 proliferation index in glioblastoma multiforme.}},
author = {{Persson, Annette and Englund, Elisabet}},
issn = {{0722-5091}},
keywords = {{Ki-67 Antigen: biosynthesis; Glioblastoma: pathology; Glioblastoma: mortality; Glioblastoma: metabolism; Brain Neoplasms: pathology; Brain Neoplasms: metabolism; Brain Neoplasms: mortality; Telomerase: biosynthesis; Tumor Markers; Biological: analysis}},
language = {{eng}},
number = {{4}},
pages = {{224--233}},
publisher = {{Dustri-Verlag}},
series = {{Clinical Neuropathology}},
title = {{Different assessments of immunohistochemically stained Ki-67 and hTERT in glioblastoma multiforme yield variable results: a study with reference to survival prognosis.}},
url = {{http://www.ncbi.nlm.nih.gov/pubmed/18666438?dopt=Abstract}},
volume = {{27}},
year = {{2008}},
}