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Female mice are more susceptible to developing inflammatory disorders due to impaired transforming growth factor beta signaling in salivary glands

Nandula, Seshagiri R.; Amarnath, Shoba; Molinolo, Alfredo; Bandyopadhyay, Bidhan C.; Hall, Bradford; Goldsmith, Corinne M.; Zheng, Changyu; Larsson, Jonas LU ; Sreenath, Taduru and Chen, WanJun, et al. (2007) In Arthritis and Rheumatism 56(6). p.1798-1805
Abstract
Objective. Transforming growth factor 13 (TGF)3) plays a key role in the onset and resolution of autoimmune diseases and chronic inflammation. The aim of this study was to delineate the precise function of TGF beta signaling in salivary gland inflammation. Methods. We impaired TGF beta signaling in mouse salivary glands by conditionally inactivating expression of TGF beta receptor type I (TGF beta RI), either by using mouse mammary tumor virus-Cre mice or by delivering adenoviral vector containing Cre to mouse salivary glands via retrograde infusion of the cannulated main excretory ducts of submandibular glands. Results. TGF beta RI-conditional knockout (TGF beta RI-coko) mice were born normal; however, female TGF beta RI-coko mice... (More)
Objective. Transforming growth factor 13 (TGF)3) plays a key role in the onset and resolution of autoimmune diseases and chronic inflammation. The aim of this study was to delineate the precise function of TGF beta signaling in salivary gland inflammation. Methods. We impaired TGF beta signaling in mouse salivary glands by conditionally inactivating expression of TGF beta receptor type I (TGF beta RI), either by using mouse mammary tumor virus-Cre mice or by delivering adenoviral vector containing Cre to mouse salivary glands via retrograde infusion of the cannulated main excretory ducts of submandibular glands. Results. TGF beta RI-conditional knockout (TGF beta RI-coko) mice were born normal; however, female TGF beta RI-coko mice developed severe multifocal inflammation in salivary and mammary glands and in the heart. The inflammatory disorder affected normal growth and resulted in the death of the mice at ages 4-5 weeks. Interestingly, male TGF beta RI-coko mice did not exhibit any signs of inflammation. The female TGF beta RI-coko mice also showed an increase in Th1 proinflammatory cytokines in salivary glands and exhibited an up-regulation of peripheral T cells. In addition, these mice showed an atypical distribution of aquaporin 5 in their salivary glands, suggesting likely secretory impairment. Administration of an adenoviral vector encoding Cre recombinase into the salivary glands resulted in inflammatory foci only in the glands of female TGF beta RI-loxP-flanked (floxed) mice (TGF beta RI-f/f mice), but not in those of male and female wild-type mice or male TGF beta RI-f/f mice. Conclusion. These results suggest that female mice are uniquely more susceptible to developing inflammatory disorders due to impaired TGF beta signaling in their salivary glands. (Less)
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published
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Arthritis and Rheumatism
volume
56
issue
6
pages
1798 - 1805
publisher
John Wiley & Sons
external identifiers
  • wos:000247164300009
  • scopus:34447526525
ISSN
1529-0131
DOI
10.1002/art.22715
language
English
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yes
id
35ff0f3c-4cd6-448c-9e10-2d02c1c15566 (old id 649002)
date added to LUP
2007-12-13 16:17:37
date last changed
2017-01-01 04:44:11
@article{35ff0f3c-4cd6-448c-9e10-2d02c1c15566,
  abstract     = {Objective. Transforming growth factor 13 (TGF)3) plays a key role in the onset and resolution of autoimmune diseases and chronic inflammation. The aim of this study was to delineate the precise function of TGF beta signaling in salivary gland inflammation. Methods. We impaired TGF beta signaling in mouse salivary glands by conditionally inactivating expression of TGF beta receptor type I (TGF beta RI), either by using mouse mammary tumor virus-Cre mice or by delivering adenoviral vector containing Cre to mouse salivary glands via retrograde infusion of the cannulated main excretory ducts of submandibular glands. Results. TGF beta RI-conditional knockout (TGF beta RI-coko) mice were born normal; however, female TGF beta RI-coko mice developed severe multifocal inflammation in salivary and mammary glands and in the heart. The inflammatory disorder affected normal growth and resulted in the death of the mice at ages 4-5 weeks. Interestingly, male TGF beta RI-coko mice did not exhibit any signs of inflammation. The female TGF beta RI-coko mice also showed an increase in Th1 proinflammatory cytokines in salivary glands and exhibited an up-regulation of peripheral T cells. In addition, these mice showed an atypical distribution of aquaporin 5 in their salivary glands, suggesting likely secretory impairment. Administration of an adenoviral vector encoding Cre recombinase into the salivary glands resulted in inflammatory foci only in the glands of female TGF beta RI-loxP-flanked (floxed) mice (TGF beta RI-f/f mice), but not in those of male and female wild-type mice or male TGF beta RI-f/f mice. Conclusion. These results suggest that female mice are uniquely more susceptible to developing inflammatory disorders due to impaired TGF beta signaling in their salivary glands.},
  author       = {Nandula, Seshagiri R. and Amarnath, Shoba and Molinolo, Alfredo and Bandyopadhyay, Bidhan C. and Hall, Bradford and Goldsmith, Corinne M. and Zheng, Changyu and Larsson, Jonas and Sreenath, Taduru and Chen, WanJun and Ambudkar, Indu S. and Karlsson, Stefan and Baum, Bruce J. and Kulkarni, Ashok B.},
  issn         = {1529-0131},
  language     = {eng},
  number       = {6},
  pages        = {1798--1805},
  publisher    = {John Wiley & Sons},
  series       = {Arthritis and Rheumatism},
  title        = {Female mice are more susceptible to developing inflammatory disorders due to impaired transforming growth factor beta signaling in salivary glands},
  url          = {http://dx.doi.org/10.1002/art.22715},
  volume       = {56},
  year         = {2007},
}