Human prostatic acid phosphatase: cDNA cloning, gene mapping and protein sequence homology with lysosomal acid phosphatase
(1989) In Biochem Biophys Res Commun 160(1). p.79-86- Abstract
- The cDNAs encoding human prostatic acid phosphatase were cloned and characterized. The mRNAs contain 3' noncoding regions of heterogeneous sizes 646, 1887 or 1913 nucleotides. A dimer and a monomer of the conserved Alu-repeats are present in the longer 3' noncoding sequences. The complete sequence of 354 amino acids for the mature enzyme was determined by sequencing both cDNA and protein. Human prostatic and lysosomal acid phosphatases exhibit 50% sequence homology, including five Cys residues and two putative N-linked glycosylation sites. The Acp-3 gene coding for human prostatic acid phosphatase was mapped onto chromosome 3 in this investigation. The Acp-2 gene coding for lysosomal acid phosphatase has previously been located on... (More)
- The cDNAs encoding human prostatic acid phosphatase were cloned and characterized. The mRNAs contain 3' noncoding regions of heterogeneous sizes 646, 1887 or 1913 nucleotides. A dimer and a monomer of the conserved Alu-repeats are present in the longer 3' noncoding sequences. The complete sequence of 354 amino acids for the mature enzyme was determined by sequencing both cDNA and protein. Human prostatic and lysosomal acid phosphatases exhibit 50% sequence homology, including five Cys residues and two putative N-linked glycosylation sites. The Acp-3 gene coding for human prostatic acid phosphatase was mapped onto chromosome 3 in this investigation. The Acp-2 gene coding for lysosomal acid phosphatase has previously been located on chromosome 11, while the Acp-1 gene coding for red blood cell acid phosphatase is on chromosome 2. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3965181
- author
- Sharief, F. S. ; Lee, H. ; Leuderman, M. M. ; Lundwall, Åke LU ; Deaven, L. L. ; Lee, C. L. and Li, S. S.
- publishing date
- 1989
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Macromolecular Substances, Lysosomes/*enzymology, Humans, Glycosylation, DNA/*genetics, Comparative Study, Molecular, *Cloning, Pair 3, Human, Chromosomes, *Chromosome Mapping, Base Sequence, Acid Phosphatase/*genetics, Amino Acid Sequence, Male, Molecular Sequence Data, Nucleic Acid Hybridization, Prostate/*enzymology, RNA, Messenger/genetics, Sequence Homology, Nucleic Acid
- in
- Biochem Biophys Res Commun
- volume
- 160
- issue
- 1
- pages
- 79 - 86
- publisher
- Elsevier
- external identifiers
-
- scopus:0024543660
- language
- English
- LU publication?
- no
- additional info
- 1
- id
- 65094a41-c096-4c63-9e5a-f3d6d589f7b3 (old id 3965181)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=2712834
- date added to LUP
- 2016-04-04 13:39:02
- date last changed
- 2021-08-29 03:34:44
@article{65094a41-c096-4c63-9e5a-f3d6d589f7b3, abstract = {{The cDNAs encoding human prostatic acid phosphatase were cloned and characterized. The mRNAs contain 3' noncoding regions of heterogeneous sizes 646, 1887 or 1913 nucleotides. A dimer and a monomer of the conserved Alu-repeats are present in the longer 3' noncoding sequences. The complete sequence of 354 amino acids for the mature enzyme was determined by sequencing both cDNA and protein. Human prostatic and lysosomal acid phosphatases exhibit 50% sequence homology, including five Cys residues and two putative N-linked glycosylation sites. The Acp-3 gene coding for human prostatic acid phosphatase was mapped onto chromosome 3 in this investigation. The Acp-2 gene coding for lysosomal acid phosphatase has previously been located on chromosome 11, while the Acp-1 gene coding for red blood cell acid phosphatase is on chromosome 2.}}, author = {{Sharief, F. S. and Lee, H. and Leuderman, M. M. and Lundwall, Åke and Deaven, L. L. and Lee, C. L. and Li, S. S.}}, keywords = {{Macromolecular Substances; Lysosomes/*enzymology; Humans; Glycosylation; DNA/*genetics; Comparative Study; Molecular; *Cloning; Pair 3; Human; Chromosomes; *Chromosome Mapping; Base Sequence; Acid Phosphatase/*genetics; Amino Acid Sequence; Male; Molecular Sequence Data; Nucleic Acid Hybridization; Prostate/*enzymology; RNA; Messenger/genetics; Sequence Homology; Nucleic Acid}}, language = {{eng}}, number = {{1}}, pages = {{79--86}}, publisher = {{Elsevier}}, series = {{Biochem Biophys Res Commun}}, title = {{Human prostatic acid phosphatase: cDNA cloning, gene mapping and protein sequence homology with lysosomal acid phosphatase}}, url = {{http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=2712834}}, volume = {{160}}, year = {{1989}}, }