Advanced

Perilipin and adipophilin expression in lipid loaded macrophages

Persson, Jenny LU ; Degerman, Eva LU ; Nilsson, Jan LU and Lindholm, Marie LU (2007) In Biochemical and Biophysical Research Communications 363(4). p.1020-1026
Abstract
Lipid-filled macrophages (foam cells) are a defining feature of atherosclerotic plaques. Foam cells contain lipid dro plet- associated proteins that in other cell types regulate lipid turnover. In foam cell such proteins may directly affect lipid droplet formation and lipid efflux. Differentiated primary human monocytes or THP-1 cells were lipid loaded by incubation with aggregated low density lipoproteins (AgLDL) or VLDL resulting in macrophage foam cells with predominantly cholesterol ester or triglyceride-rich lipid droplets, respectively. Lipid droplets were isolated and major proteins identified by mass spectrometry, among them the apolipoprotein B-48 receptor that has not previously been recognized in this context. Expression of two... (More)
Lipid-filled macrophages (foam cells) are a defining feature of atherosclerotic plaques. Foam cells contain lipid dro plet- associated proteins that in other cell types regulate lipid turnover. In foam cell such proteins may directly affect lipid droplet formation and lipid efflux. Differentiated primary human monocytes or THP-1 cells were lipid loaded by incubation with aggregated low density lipoproteins (AgLDL) or VLDL resulting in macrophage foam cells with predominantly cholesterol ester or triglyceride-rich lipid droplets, respectively. Lipid droplets were isolated and major proteins identified by mass spectrometry, among them the apolipoprotein B-48 receptor that has not previously been recognized in this context. Expression of two proteins, perilipin and adipophilin, was quantified by Western blots of cell lysates. Perilipin content decreased and adipophilin increased with lipoprotein lipid loading regardless of intracellular neutral lipid composition. This protein expression pattern may hinder lipid turnover in macrophage foam cells, thereby increasing lipid content of atherosclerotic plaques. (C) 2007 Elsevier Inc. All rights reserved. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
PAT proteins, lipid droplet, foam cell, macrophage
in
Biochemical and Biophysical Research Communications
volume
363
issue
4
pages
1020 - 1026
publisher
Elsevier
external identifiers
  • wos:000250456400024
  • scopus:35248899411
ISSN
1090-2104
DOI
10.1016/j.bbrc.2007.09.074
language
English
LU publication?
yes
id
abcc006f-27de-47ce-81de-350c4bd2077c (old id 653256)
date added to LUP
2007-12-18 14:04:34
date last changed
2017-09-24 04:24:55
@article{abcc006f-27de-47ce-81de-350c4bd2077c,
  abstract     = {Lipid-filled macrophages (foam cells) are a defining feature of atherosclerotic plaques. Foam cells contain lipid dro plet- associated proteins that in other cell types regulate lipid turnover. In foam cell such proteins may directly affect lipid droplet formation and lipid efflux. Differentiated primary human monocytes or THP-1 cells were lipid loaded by incubation with aggregated low density lipoproteins (AgLDL) or VLDL resulting in macrophage foam cells with predominantly cholesterol ester or triglyceride-rich lipid droplets, respectively. Lipid droplets were isolated and major proteins identified by mass spectrometry, among them the apolipoprotein B-48 receptor that has not previously been recognized in this context. Expression of two proteins, perilipin and adipophilin, was quantified by Western blots of cell lysates. Perilipin content decreased and adipophilin increased with lipoprotein lipid loading regardless of intracellular neutral lipid composition. This protein expression pattern may hinder lipid turnover in macrophage foam cells, thereby increasing lipid content of atherosclerotic plaques. (C) 2007 Elsevier Inc. All rights reserved.},
  author       = {Persson, Jenny and Degerman, Eva and Nilsson, Jan and Lindholm, Marie},
  issn         = {1090-2104},
  keyword      = {PAT proteins,lipid droplet,foam cell,macrophage},
  language     = {eng},
  number       = {4},
  pages        = {1020--1026},
  publisher    = {Elsevier},
  series       = {Biochemical and Biophysical Research Communications},
  title        = {Perilipin and adipophilin expression in lipid loaded macrophages},
  url          = {http://dx.doi.org/10.1016/j.bbrc.2007.09.074},
  volume       = {363},
  year         = {2007},
}