Perilipin and adipophilin expression in lipid loaded macrophages
(2007) In Biochemical and Biophysical Research Communications 363(4). p.1020-1026- Abstract
- Lipid-filled macrophages (foam cells) are a defining feature of atherosclerotic plaques. Foam cells contain lipid dro plet- associated proteins that in other cell types regulate lipid turnover. In foam cell such proteins may directly affect lipid droplet formation and lipid efflux. Differentiated primary human monocytes or THP-1 cells were lipid loaded by incubation with aggregated low density lipoproteins (AgLDL) or VLDL resulting in macrophage foam cells with predominantly cholesterol ester or triglyceride-rich lipid droplets, respectively. Lipid droplets were isolated and major proteins identified by mass spectrometry, among them the apolipoprotein B-48 receptor that has not previously been recognized in this context. Expression of two... (More)
- Lipid-filled macrophages (foam cells) are a defining feature of atherosclerotic plaques. Foam cells contain lipid dro plet- associated proteins that in other cell types regulate lipid turnover. In foam cell such proteins may directly affect lipid droplet formation and lipid efflux. Differentiated primary human monocytes or THP-1 cells were lipid loaded by incubation with aggregated low density lipoproteins (AgLDL) or VLDL resulting in macrophage foam cells with predominantly cholesterol ester or triglyceride-rich lipid droplets, respectively. Lipid droplets were isolated and major proteins identified by mass spectrometry, among them the apolipoprotein B-48 receptor that has not previously been recognized in this context. Expression of two proteins, perilipin and adipophilin, was quantified by Western blots of cell lysates. Perilipin content decreased and adipophilin increased with lipoprotein lipid loading regardless of intracellular neutral lipid composition. This protein expression pattern may hinder lipid turnover in macrophage foam cells, thereby increasing lipid content of atherosclerotic plaques. (C) 2007 Elsevier Inc. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/653256
- author
- Persson, Jenny
LU
; Degerman, Eva
LU
; Nilsson, Jan LU and Lindholm, Marie LU
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- PAT proteins, lipid droplet, foam cell, macrophage
- in
- Biochemical and Biophysical Research Communications
- volume
- 363
- issue
- 4
- pages
- 1020 - 1026
- publisher
- Elsevier
- external identifiers
-
- wos:000250456400024
- scopus:35248899411
- ISSN
- 1090-2104
- DOI
- 10.1016/j.bbrc.2007.09.074
- language
- English
- LU publication?
- yes
- id
- abcc006f-27de-47ce-81de-350c4bd2077c (old id 653256)
- date added to LUP
- 2016-04-01 16:14:19
- date last changed
- 2022-01-28 18:17:39
@article{abcc006f-27de-47ce-81de-350c4bd2077c, abstract = {{Lipid-filled macrophages (foam cells) are a defining feature of atherosclerotic plaques. Foam cells contain lipid dro plet- associated proteins that in other cell types regulate lipid turnover. In foam cell such proteins may directly affect lipid droplet formation and lipid efflux. Differentiated primary human monocytes or THP-1 cells were lipid loaded by incubation with aggregated low density lipoproteins (AgLDL) or VLDL resulting in macrophage foam cells with predominantly cholesterol ester or triglyceride-rich lipid droplets, respectively. Lipid droplets were isolated and major proteins identified by mass spectrometry, among them the apolipoprotein B-48 receptor that has not previously been recognized in this context. Expression of two proteins, perilipin and adipophilin, was quantified by Western blots of cell lysates. Perilipin content decreased and adipophilin increased with lipoprotein lipid loading regardless of intracellular neutral lipid composition. This protein expression pattern may hinder lipid turnover in macrophage foam cells, thereby increasing lipid content of atherosclerotic plaques. (C) 2007 Elsevier Inc. All rights reserved.}}, author = {{Persson, Jenny and Degerman, Eva and Nilsson, Jan and Lindholm, Marie}}, issn = {{1090-2104}}, keywords = {{PAT proteins; lipid droplet; foam cell; macrophage}}, language = {{eng}}, number = {{4}}, pages = {{1020--1026}}, publisher = {{Elsevier}}, series = {{Biochemical and Biophysical Research Communications}}, title = {{Perilipin and adipophilin expression in lipid loaded macrophages}}, url = {{http://dx.doi.org/10.1016/j.bbrc.2007.09.074}}, doi = {{10.1016/j.bbrc.2007.09.074}}, volume = {{363}}, year = {{2007}}, }